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Mouse vcp sirna

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Mouse VCP siRNA is a laboratory reagent designed to temporarily suppress the expression of the VCP (Valosin-Containing Protein) gene in mouse cells. VCP is a protein involved in various cellular processes. The siRNA (small interfering RNA) molecule targets the VCP mRNA, leading to its degradation and reduced VCP protein levels.

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Lab products found in correlation

Transit tko transfection reagent, by Mirus Bio (2 mentions) Control sirna, by Thermo Fisher Scientific (2 mentions) Control sirna, by Mirus Bio (2 mentions)

Spelling variants of this product

SiRNAs (258 citations) VCP siRNA (2 citations)

2 protocols using mouse vcp sirna

1

siRNA Knockdown of VCP and UBXD1 in HdhQ111 Cells

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Control siRNA, mouse VCP siRNA, and mouse UBXD1 siRNA were purchased from Thermo Fisher Scientific. HdhQ111 cells were transfected either with Control siRNA or VCP siRNA or UBXD1 siRNA using TransIT-TKO® Transfection Reagent (Mirus Bio LLC), according to the manufacturer’s instructions.
Guo X, & Qi X. (2016). VCP cooperates with UBXD1 to degrade mitochondrial outer membrane protein MCL1 in model of Huntington’s disease. Biochimica et biophysica acta, 1863(2), 552-559.
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2

Silencing Htt and VCP in HD Cells

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For silencing Htt and VCP in HD striatal cells, control siRNA, mouse Htt and mouse VCP siRNA were purchased from Thermo Fisher Scientific. HdhQ7 and HdhQ111 cells were transfected either with control siRNA, or Htt or VCP siRNA using TransIT-TKO Transfection Reagent (Mirus Bio, LLC), according to the manufacturer's instructions. The sequences for the siRNAs used in this study are as follows: mouse Htt, 5′-GGUUUAUGAACUGACUUUGTT-3′; Mouse VCP, 5′-GAGAGCAACCUUCGUAAG-3′; control non-targeting siRNA, 5′-TTCTCCGAACGTGTCACGT-3′.
Guo X., Sun X., Hu D., Wang Y.J., Fujioka H., Vyas R., Chakrapani S., Joshi A.U., Luo Y., Mochly-Rosen D, & Qi X. (2016). VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington's disease. Nature Communications, 7, 12646.
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