C57BL/6 or KK.Cg‐Ay/J mice aged 10 weeks were obtained from the Jackson Laboratory (Bar Harbor, ME). The procedures for creating our modified CaCl2‐induced mouse model of aneurysm were described previously.13 We applied the procedure for the infrarenal aorta to the carotid artery in this study. Briefly, 0.5 mol/L CaCl2‐soaked gauze was applied perivascularly for 10 minutes to the carotid artery. The gauze was replaced with PBS‐soaked gauze for 5 minutes, and the incised area was sutured. The excess CaCl2 is converted into CaPO4, and the crystals act as adjuvants. After 1 to 4 weeks, the mice were sacrificed, and the carotid arteries were collected after fixing by perfusion with 4% paraformaldehyde. The diameter of the artery at the time of the initial surgery and sacrifice was measured with an electronic digital caliper (VWR International). All animal procedures were conducted in accordance with experimental protocols that were approved by the institutional animal care and use committee at the University of Wisconsin, Madison (protocol M02394).
Electronic digital caliper
Manufactured by Avantor
Sourced in United States
The electronic digital caliper is a precision measuring instrument used to measure the dimensions of objects. It features a digital display that provides accurate measurements, often with a resolution of up to 0.01 mm. The caliper typically includes a main scale, a vernier scale, and a locking mechanism to secure the measurement.
Automatically generated - may contain errors
4 protocols using electronic digital caliper
1
CaCl2-Induced Carotid Artery Aneurysm
2
Protective Immunogenicity of DNA and Recombinant Protein Vaccine Regimens against Leishmania major Infection in BALB/c Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After two weeks of acclimatization, five female BALB/c mice per group were injected with DNA vaccine antigens or controls. Two DNA immunizations were given at week-0 and week-3 followed by one recombinant protein boost at week-6. All immunizations were prepared in 50 µl solution in endotoxin-free PBS (Teknova, USA). DNA immunization was performed by intramuscular injection of a mixture of 100 µg plasmid DNA and 25 µg CpG ODN in 50 µl total volume. The recombinant protein booster immunization was given to the vaccine groups by subcutaneous injection (SC) of 12.5 µg rLdPxn1 protein in combination with 25 µg CpG ODN in the right hind footpad. All the three injections to mice that received pcDNA and pcDNA-mGMCSF were given in the form of plasmid DNA only.
Leishmania major strain V1 (MHOM/IL/80/Friedlin) was used for the protection study in BALB/c mice. Stationary phase promastigotes, cultured in M199 medium with 20% FBS, were washed and resuspended in endotoxin-free PBS. 3×106 stationary phase live promastigotes in 40 µl endotoxin-free PBS were injected subcutaneously into the hind left footpad of each mouse. The thickness of the footpads was then measured weekly until euthanasia using an electronic digital caliper (VWR, USA). Mice that showed a net footpad swelling of more than 3 mm thick or those that developed necrotic lesions were euthanized even before the end date of week-17.
Leishmania major strain V1 (MHOM/IL/80/Friedlin) was used for the protection study in BALB/c mice. Stationary phase promastigotes, cultured in M199 medium with 20% FBS, were washed and resuspended in endotoxin-free PBS. 3×106 stationary phase live promastigotes in 40 µl endotoxin-free PBS were injected subcutaneously into the hind left footpad of each mouse. The thickness of the footpads was then measured weekly until euthanasia using an electronic digital caliper (VWR, USA). Mice that showed a net footpad swelling of more than 3 mm thick or those that developed necrotic lesions were euthanized even before the end date of week-17.
3
Murine Arterial Aneurysm Model with CaCl2 and CSE
4
Antimicrobial Susceptibility of Streptococcus mutans
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Streptococcus mutans CCUG 11877 was grown on mitis salivarius-bacitracin (MSB) agar at 37°C in 5% CO 2 in air for 2 days. Colonies were harvested with a disposable sterile plastic loop and suspended in sterile phosphatebuffered saline (PBS). The cells were washed twice by centrifugation at 5,000 × g for 5 min. The Optical density at 600 nm was adjusted to 1, and a 10-fold dilution was prepared, from which 100 µL was spread on Mueller-Hinton agar plates. One blank antimicrobial susceptible disk was placed in the center of each plate, and 20 µL of SDF [38% weight/volume (w/v)] or SDF + KI (SDF application followed by KI in sequence, not as a mixture. KI was applied as a saturated solution of KI and an oxalic acid-based product containing oxalic acid, potassium, salt, and water) (SDI Ltd., Bayswater, Australia). Sterile saline (0.9% w/v sodium chloride) and 2% CHX (Ultradent Products Inc. South Jordan, UT, USA) were used as negative and positive controls, respectively. The plates were incubated as described above for 2-3 days, and zones of inhibition were measured using an electronic digital caliper (VWR, Radnor, PA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!