Dp21 sal digital camera

Blood smeared slides were fixed in absolute methanol for 2 min and stained with a 10% Giemsa solution (Merck, Germany) in phosphate buffer (pH 7.2) for 30 min. Slides were later washed with clean water and screened at 400× magnification. The slides were examined at 1,000× magnification under a light microscope (Olympus C×31, Tokyo, Japan) for a minimum of 20–25 min (Valkiunas et al., 2008 (link)). Parasitemia level was recorded as percentage of infected erythrocyte in a total number of erythrocytes. The calculation used as follows: total number of infected erythrocytes/average of erythrocyte*number of observed field (Schaer et al., 2015 (link)). In total, 20–100 fields were observed in each blood film. The average of erythrocytes per field was estimated by counting one to three fields with equal density. The negative blood smears were examined at least two times to avoid false negative. The images were recorded with Olympus DP21-SAL digital camera (Tokyo, Japan). Identification of haemosporidian parasites was based on descriptions or images from Garnham (1966) , Duval et al. (2012) (link), and Schaer et al. (2013) (link).