Ls magnetic separation columns

Manufactured by Miltenyi Biotec

The LS magnetic separation columns from Miltenyi Biotec are designed for the isolation of magnetically labeled cells or other target molecules. The columns utilize a strong magnetic field to capture and retain the labeled targets, allowing for the separation and purification of the desired components from the sample.

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Lab products found in correlation

Cd11b microbead, by Miltenyi Biotec (1 mentions) Neuron isolation kit, by Miltenyi Biotec (1 mentions)

Close spelling variants of this product

Magnetic separation LS column MACS magnetic LS separation columns LS separation columns LS magnetic column Magnetic separation columns LS columns Separation columns Magnetic separation Magnetic column

2 protocols using ls magnetic separation columns

Monocyte Tracking in Tumor Microenvironment

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Monocyte tracking was adapted and modified from Qian et al^{8 (link)}. Bone marrow cells were isolated from approximately 80 day old wild-type FVB/N virgin females. CD11b⁺ cells were isolated with CD11b MicroBeads (Miltenyi Biotec 130-049-601) using LS magnetic separation columns (Miltenyi Biotec 130-042-401) per manufacturer’s instruction. CD11b⁺ cells were incubated with 10 μM of CFSE (ThermoFisher C34554) for 15 minutes at 37°C. Cells were washed and injected IV into mice who had been treated for one day with DMSO or TMP195. Mice were treated for an additional 5 days and tumors were harvested. The percent of CD11b⁺CFSE⁺ double positive cells was assessed by flow cytometry.

Guerriero J.L., Sotayo A., Ponichtera H.E., Castrillon J.A., Pourzia A.L., Schad S., Johnson S.F., Carrasco R.D., Lazo S., Bronson R.T., Davis S.P., Lobera M., Nolan M.A, & Letai A. (2017). Class IIa HDAC inhibition reduces breast tumors and metastases via anti-tumor macrophages. Nature, 543(7645), 428-432.

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Purification of Microglia and Neurons from Adult Mouse Cortex

Cited 1 time

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Microglia and neurons were purified from cortices from adult (8 weeks) tmTNF^Δ/Δ and tmTNF^wt/wt mice by MACS affinity-based cell sorting (Miltenyi Biotech, Bergisch-Gladbach, Germany) as previously described (Clausen et al., 2016b (link)). Mice were sacrificed by cervical dislocation, cortices dissociated with Neuronal dissociation papain kit (P) (Miltenyi Biotech) and microglia and neurons purified according to the manufacturer’s protocol. The cell suspensions were incubated with monoclonal CD11b beads (Miltenyi Biotech) and applied to LS magnetic separation columns (Miltenyi Biotech) attached to a magnetic field for positive selection of microglia. The flow through was further incubated with Neuron Isolation Kit (Miltenyi Biotech) and applied to LD magnetic separation columns (Miltenyi Biotech) for negative selection of neurons. The cell pellets were stored at −80°C until preparation for mass spectrometry.

Yli-Karjanmaa M., Clausen B.H., Degn M., Novrup H.G., Ellman D.G., Toft-Jensen P., Szymkowski D.E., Stensballe A., Meyer M., Brambilla R, & Lambertsen K.L. (2019). Topical Administration of a Soluble TNF Inhibitor Reduces Infarct Volume After Focal Cerebral Ischemia in Mice. Frontiers in Neuroscience, 13, 781.

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