Truseq exome enrichment kit

Manufactured by Agilent Technologies

The TruSeq exome enrichment kit is a targeted sequencing solution designed to capture the protein-coding regions of the human genome, known as the exome. The kit utilizes hybridization-based enrichment technology to selectively sequence the exonic regions, providing a comprehensive and efficient approach to analyzing the functional portion of the genome.

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Lab products found in correlation

Sureselect exome enrichment kit, by Agilent Technologies (2 mentions) Hiseq 2500, by Illumina (2 mentions) Hiseq 2000, by Illumina (2 mentions) Taqman snp genotyping assay, by Thermo Fisher Scientific (1 mentions) Sureselectxt human all exon v4, by Agilent Technologies (1 mentions) Abi 3730xl dna analyser, by Thermo Fisher Scientific (1 mentions) Hiseq 2000 instrument, by Illumina (1 mentions) Miseq sequencing platform, by Illumina (1 mentions) Viia 7 real time pcr system, by Thermo Fisher Scientific (1 mentions)

3 protocols using truseq exome enrichment kit

Targeted Exome Sequencing and Validation of CCNF Mutations

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Exomes were captured using TruSeq Exome Enrichment kit or Agilent SureSelectXT Human All Exon V4. Paired-end sequencing was performed using the Illumina HiSeq2000 instrument.
CCNF exons were sequenced using Fluidigm Access Array target enrichment and the MiSeq sequencing platform (Illumina). Amplicon primers were designed using the Fluidigim D3 Design Studio to produce 150 bp amplicons targeting the exons of CCNF.
Validation and analysis of the CCNF mutations was performed by direct DNA sequencing following PCR amplification of coding exons (NM_001761). PCR products were Sanger sequenced using Big-Dye terminator sequencing and an ABI 3730XL DNA analyser (Applied Biosystems).
SNP genotyping in control individuals was performed using a custom TaqMan SNP genotyping assay according to the manufacturer's instructions (Life Technologies) and analysed using a Viia 7 real-time PCR system (Life Technologies).
Control exome data from 967 neurologically healthy individuals of predominantly Western European descent obtained from the Diamantina Institute, University of Queensland (Diamantina Australian Control Collection).

Williams K.L., Topp S., Yang S., Smith B., Fifita J.A., Warraich S.T., Zhang K.Y., Farrawell N., Vance C., Hu X., Chesi A., Leblond C.S., Lee A., Rayner S.L., Sundaramoorthy V., Dobson-Stone C., Molloy M.P., van Blitterswijk M., Dickson D.W., Petersen R.C., Graff-Radford N.R., Boeve B.F., Murray M.E., Pottier C., Don E., Winnick C., McCann E.P., Hogan A., Daoud H., Levert A., Dion P.A., Mitsui J., Ishiura H., Takahashi Y., Goto J., Kost J., Gellera C., Gkazi A.S., Miller J., Stockton J., Brooks W.S., Boundy K., Polak M., Muñoz-Blanco J.L., Esteban-Pérez J., Rábano A., Hardiman O., Morrison K.E., Ticozzi N., Silani V., de Belleroche J., Glass J.D., Kwok J.B., Guillemin G.J., Chung R.S., Tsuji S., Brown RH J.r., García-Redondo A., Rademakers R., Landers J.E., Gitler A.D., Rouleau G.A., Cole N.J., Yerbury J.J., Atkin J.D., Shaw C.E., Nicholson G.A, & Blair I.P. (2016). CCNF mutations in amyotrophic lateral sclerosis and frontotemporal dementia. Nature Communications, 7, 11253.

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Exome Sequencing with High Coverage

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Exome capture was performed according to the manufacturer's protocol using either Illumina's TruSeq exome enrichment kit (11 paired samples) or Agilent's SureSelect exome enrichment kit (1 paired sample). The equivalent of 1 to 3 exomes was sequenced per lane on an Illumina HiSeq2000 or HiSeq2500 sequencer for an average of 90-fold coverage of coding regions after removing duplicated reads (range: 30×-199×). FASTQ files are available in the NCBI SRA database (SRP074519).

Bouska A., Zhang W., Gong Q., Iqbal J., Scuto A., Vose J., Ludvigsen M., Fu K., Weisenburger D.D., Greiner T.C., Gascoyne R.D., Rosenwald A., Ott G., Campo E., Rimsza L.M., Delabie J., Jaffe E.S., Braziel R.M., Connors J.M., Wu C.I., Staudt L.M., D'Amore F., McKeithan T.W, & Chan W.C. (2016). Combined copy number and mutation analysis identifies oncogenic pathways associated with transformation of follicular lymphoma. Leukemia, 31(1), 83-91.

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Exome Sequencing with High Coverage

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