Mb 200b

Manufactured by MBraun

The MB 200B is a compact and versatile glove box system designed for controlled atmosphere applications. It features a dual-chamber design with an antechamber for sample introduction and a main chamber for experimental work. The system is constructed using high-quality stainless steel and includes integrated gas purification to maintain a stable, low-oxygen and low-moisture environment.

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Lab products found in correlation

Titrando 901, by Metrohm (2 mentions) Escalab 250xi, by Thermo Fisher Scientific (2 mentions) Tecnai g2 f20 microscope, by Thermo Fisher Scientific (1 mentions) D8 advance, by Brucker (1 mentions) S 4800, by Hitachi (1 mentions) Labram raman spectrometer, by Horiba (1 mentions) Clarus 500, by PerkinElmer (1 mentions)

Close spelling variants of this product

MB 200B Eco

5 protocols using mb 200b

Water Adsorption Isotherms on Molecular Sieve

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Isotherms for water
adsorption from saturated dichloromethane (VWR, The Netherlands) onto
molecular sieve 3A beads, 2 mm diameter (Sigma–Aldrich), were
measured at 25 and 40 °C. Molecular sieves were dried in an oven
at 200 °C for 48 h. Adsorbent was then cooled to room temperature
in a glovebox (MBraun MB 200B) kept in a dry nitrogen atmosphere (<0.3
ppm of water). Between 0.02 and 0.50 g of dry adsorbent was weighed
and transferred into 60 mL vials capped with PTFE septum caps. 60
g of DCM saturated with demineralized water (Veolia Water Elga Purelab
S7, 0.1 μS cm^–1) was injected into the same
vials through the septum. A dry DCM sample was prepared in the same
manner to detect potential contamination of the samples with water.
The prepared samples were then placed inside a shaker (IKA) at 100
rpm at a preset temperature. Samples were taken at fixed time intervals
with 2 mL syringes through the septum, to avoid water adsorption from
air. The water content was measured with a coulometric Karl Fischer
titrator (Metrohm Applikon Coulometer KFT 899). The equilibrium between
the adsorbent and the liquid was reached within 2 days. The average
mass loss of DCM was <1% (w/w).

Jović S., Laxminarayan Y., Keurentjes J., Schouten J, & van der Schaaf J. (2017). Adsorptive Water Removal from Dichloromethane and Vapor-Phase Regeneration of a Molecular Sieve 3A Packed Bed. Industrial & Engineering Chemistry Research, 56(17), 5042-5054.

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Characterization of FNDSP Catalysts

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X-ray photoelectron spectroscopy (XPS) was used to characterize the iron and nitrogen species of the synthesized FNDSP catalysts by using an X-ray photoelectron spectrometer (Thermo ESCALAB 250Xi) with Al Kα as the radiation source. The degree of carbon defects was investigated by Raman spectra and measured on a Horiba Jobin Yvon (HJY) Lab RAM Raman spectrometer. For structure characterization, X-ray diffraction (XRD) tests were carried out on an X-ray diffractometer (Brucker D8 Advance) with Cu Kα radiation. The composition and morphology of the FNDSP cathode materials were analyzed by using field emission scanning electron microscopy (FE-SEM, Hitachi S-4800). Transmission electron microscopy (TEM) and elemental mapping investigations were performed with a FEI Tecnai G2 F20 microscope operated at 200 kV. To acquire the cathode after the cycling process, the cell was dissembled in a glove box (MBraun MB 200B) filled with argon, and the cathode was soaked in pure DME for 3–5 minutes to remove residual salts of the electrolyte. After being washed 3 times, the cathode was dried in a vacuum oven and then transferred to an airtight container filled with argon for further characterization.

Zhang G., Zhang L., Zhao S., Lu S., Lu Y., Sun H, & Wang L. (2020). Principle understanding towards synthesizing Fe/N decorated carbon catalysts with pyridinic-N enriched and agglomeration-free features for lithium–oxygen batteries. RSC Advances, 10(7), 3853-3860.

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Measuring Hydrogenase Activity in Whole Cell Extracts

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Hydrogenase activity of whole cell extracts was measured in vitro, as previously described23 (link). Briefly, H₂ production from reduced methyl viologen was measured in nitrogen-flushed 13.5 ml sealed vials, using a Clarus 500 gas chromatographer equipped with an Elite-Molesieve column (0.53 mm i.d., 30 m length) (Perkin Elmer). All steps were performed under anaerobic conditions in a glove-box (MBRAUN MB 200B), using oxygen-free solutions.

De Rosa E., Checchetto V., Franchin C., Bergantino E., Berto P., Szabò I., Giacometti G.M., Arrigoni G, & Costantini P. (2015). [NiFe]-hydrogenase is essential for cyanobacterium Synechocystis sp. PCC 6803 aerobic growth in the dark. Scientific Reports, 5, 12424.

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Mineralization of Collagen with Iron

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Mineralization of collagen was performed inside a wet MBraun MB 200B glovebox under nitrogen atmosphere ([O₂] < 5 ppm, unless stated otherwise). Titration experiments were performed at room temperature with a Metrohm Titrando 901 automated titration set‐up, controlled by a computer running the software program Tiamo 2.5, and equipped with a glass pH electrode (Metrohm article number 6.0234.100) and a Dosino 10 mL dosing device.
For the mineralization of collagen via a coprecipitation procedure, a solution of ferric chloride (4 mM) and ferrous chloride (2 mM) was titrated with 0.07 M KOH at a rate of 0.01 mL min⁻¹ until pH 9 was reached. For experiments in the presence of pAsp, 7 mg mL⁻¹ pAsp (12 mM aspartic acid groups) was added to the iron solution. For experiments in the presence of dispersed collagen, the iron solution was added to the collagen to reach a final collagen concentration of 5 mg mL⁻¹. The dispersion was left standing for 400 days (13 months), after which TEM samples were prepared.

Oosterlaken B.M., Friedrich H, & de With G. (2021). The effects of washing a collagen sample prior to TEM examination. Microscopy Research and Technique, 85(1), 412-417.

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Coprecipitation of Iron Oxides with pAsp and Collagen

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Coprecipitation
experiments were performed in a wet MBraun MB 200B glovebox under
a nitrogen atmosphere [(O₂) < 5 ppm]. Titration experiments
were performed at room temperature with a Metrohm Titrando 901 automated
titration setup, controlled by a computer running software program
Tiamo 2.5, and equipped with a glass pH electrode (Metrohm article
number 6.0234.100) and a Dosino 10 mL dosing device. A solution of
ferric chloride (4 mM) and ferrous chloride (2 mM) was titrated with
0.07 M KOH at a rate of 0.01 mL min^–1 until pH 9
was reached. For experiments in the presence of pAsp, 7 mg mL^–1 pAsp (12 mM aspartic acid groups) was added to the
iron solution. For experiments in the presence of dispersed collagen,
the iron solution was added to the collagen to reach a final collagen
concentration of 5 mg mL^–1.

Oosterlaken B.M., van Rijt M.M., Joosten R.R., Bomans P.H., Friedrich H, & de With G. (2021). Time-Resolved Cryo-TEM Study on the Formation of Iron Hydroxides in a Collagen Matrix. ACS Biomaterials Science & Engineering, 7(7), 3123-3131.

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