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Tnf α

Manufactured by Beckman Coulter
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Sourced in France

The TNF-α is a lab equipment product that measures the levels of tumor necrosis factor alpha (TNF-α), a cytokine involved in systemic inflammation. This product provides a quantitative determination of TNF-α concentration in biological samples.

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Lab products found in correlation

Interleukin 2 (il 2), by BD (1 mentions) Dc sign, by BD (1 mentions) Accuri c6 cytometer, by BD (1 mentions) Sr b1, by Novus Biologicals (1 mentions) Sr a1, by R&D Systems (1 mentions) Lox 1, by BioLegend (1 mentions) Hla abc, by BioLegend (1 mentions) Cd206, by BD (1 mentions) Hla dr, by Beckman Coulter (1 mentions) Facscanto 2, by BD (1 mentions) Isotype control, by BioLegend (1 mentions) Anti human cd19, by BD (1 mentions) Tgf β1, by BioLegend (1 mentions) Ifn γ, by Beckman Coulter (1 mentions)

Spelling variants of this product

Anti-TNF-α (188; (2 citations)

3 protocols using tnf α

1

Endocytic receptor and immune cell analysis

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Endocytic receptors were stained using the following antibodies: CD206/MR (eBioscience), DC-SIGN, CD36 (both from BD Biosciences), SR-A1 (R&D Systems), LOX-1 (BioLegend), and SR-B1 (Novus Biologicals). DC and T cell phenotypes were examined using antibodies against the following markers: HLA-ABC (BioLegend), CD206, CD40, CD80, CD83, IL-2 (BD Biosciences), CD4, CD8, TNF-α, IL-2, and HLA-DR (Beckman Coulter). Data were acquired with an Accuri C6 cytometer (BD Biosciences) and analyzed using CFlow Plus software.
Pardee A.D., Yano H., Weinstein A.M., Ponce A.A., Ethridge A.D., Normolle D.P., Vujanovic L., Mizejewski G.J., Watkins S.C, & Butterfield L.H. (2015). Route of antigen delivery impacts the immunostimulatory activity of dendritic cell-based vaccines for hepatocellular carcinoma. Journal for Immunotherapy of Cancer, 3, 32.
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2

Cytokine Profiling in HSCT Patients

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Culture supernatants of peripheral blood mononuclear cells (PBMC) stimulated with phytohemagglutinin (PHA) were tested by ELISA for levels of IL-2, IL-4, TNFα (Beckman-Coulter, France) and IFN-γ (Immunotech, France) as per the manufacturer’s instructions. Samples were tested in triplicate at 24 h for IL-4 and IL-2 and at 96 h for TNFα and IFN-γ. The sensitivities of cytokine detection were 3 pg/ml for IFN-γ, 10 pg/ml for TNFα, 5 pg/ml for IL-2 and 5 pg/ml for IL-4. At the time of testing, there were no clinical evidence of acute infection or allergic reaction and none of the patients were on systemic immunosuppression. Testing was done for the same patients pre- and post-HSCT.
Al-Herz W., Chu J.I., van der Spek J., Raghupathy R., Massaad M.J., Keles S., Biggs C.M., Cockerton L., Chou J., Dbaibo G., Elisofon S.A., Hanna-Wakim R., Kim H.B., Lehmann L.E., McDonald D.R., Notarangelo L.D., Veys P., Chatila T., Geha R.S., Gaspar H.B, & Pai S.Y. (2016). Hematopoietic Stem Cell Transplantation Outcomes for 11 Patients with Dedicator of Cytokinesis 8 (DOCK8) Deficiency. The Journal of allergy and clinical immunology, 138(3), 852-859.e3.
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3

Comprehensive Immune Cell Profiling

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The following antibodies were used: anti-human CD19, CD21, CD38, TBNK 6-Color Kit and isotype controls (BD Biosciences), CD24, CD1d, IL-10, FoxP3 (eBiosciences), CD3, CD4, CD5, CD10, CD22, CD25, CD45, IFN-γ, TNF-α, IL-4 and isotype controls (all from Beckman Coulter), IgM (DAKO), TGF-β1 and isotype controls (Biolegend). Cell staining and flow cytometry analysis were performed according to routine laboratory methods, as described in the Supplementary Information. Stained cells were analyzed via flow cytometry analysis using a FACS Canto II (BD Biosciences). Data were analyzed using FlowJo software (Tree Star, Ashland, OR).
Wang W., Yuan X., Chen H., Xie G., Ma Y., Zheng Y., Zhou Y, & Shen L. (2015). CD19+CD24hiCD38hiBregs involved in downregulate helper T cells and upregulate regulatory T cells in gastric cancer. Oncotarget, 6(32), 33486-33499.
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