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Ml133

Manufactured by Merck Group
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Sourced in United States

The ML133 is a general-purpose laboratory centrifuge designed for a wide range of applications. It features a compact and durable construction, and can accommodate a variety of rotor types and sample volumes. The ML133 provides consistent and reliable performance for routine centrifugation tasks in research and clinical laboratories.

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Lab products found in correlation

Quinine, by Merck Group (1 mentions) Ab209845, by Abcam (1 mentions) β actin, by Merck Group (1 mentions) Lipofectamine 3000, by InvivoGen (1 mentions) Epr16883, by Abcam (1 mentions) G 20b 0014 c100, by Adipogen (1 mentions) Rad001, by MedChemExpress (1 mentions) Mk2206, by MedChemExpress (1 mentions) Zacopride, by Bio-Techne (1 mentions)

2 protocols using ml133

1

Inflammasome Activation Pathways

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Pharmacological reagents were obtained from the following manufacturers: Sigma (ML133, quinine, tetraethylammonium chloride (TEA), tetrapentylammonium (TPA), MCC950, lipopolysaccharide (LPS) from Escherichia coli O26:B6, and ATP), AdooQ (TRAM‐34 and PAP‐1), Alomone Labs (guangxitoxin‐1E), Tocris (dofetilide), Merck Millipore (Ac‐YVAD‐CMK), U.S Silica (Silica), Life Technologies (DNA [pEF/v5‐His A plasmid empty vector], Lipofectamine 3000), and Invivogen (ultrapure flagellin from Salmonella typhimurium and imiquimod). Specific antibodies were used targeting: mouse IL‐1β (AF‐401, R&D), caspase‐1 p10 (EPR16883, Abcam), gasdermin D (ab209845, Abcam), NLRP3 (G‐20B‐0014‐C100, Adipogen), and β‐actin (Sigma). All other materials/reagents were obtained from Sigma unless otherwise stated.
Drinkall S., Lawrence C.B., Ossola B., Russell S., Bender C., Brice N.B., Dawson L.A., Harte M, & Brough D. (2022). The two pore potassium channel THIK‐1 regulates NLRP3 inflammasome activation. Glia, 70(7), 1301-1316.
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2

Preparation of Drug Compounds

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ML133 (Sigma‐Aldrich), MK2206 and RAD001 (MedChemExpress, USA) were dissolved in DMSO at working concentrations of 20, 5 and 10 nM, respectively. In addition, zacopride (Tocris Bioscience) was dissolved in pure water, and bacteria were removed with 0.22‐µm filter membranes.
Cui X., Li X., He Y., Yu J., Dong N, & Zhao R.C. (2021). Slight up‐regulation of Kir2.1 channel promotes endothelial progenitor cells to transdifferentiate into a pericyte phenotype by Akt/mTOR/Snail pathway. Journal of Cellular and Molecular Medicine, 25(21), 10088-10100.
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