Fitc conjugated a 629511

Manufactured by Thermo Fisher Scientific

Sourced in United States

FITC-conjugated (A-629511) is a fluorescently labeled reagent used in various laboratory applications. It serves as a detection tool for identifying and analyzing specific biological targets.

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Lab products found in correlation

Arginase 1, by Santa Cruz Biotechnology (1 mentions) F4 80, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions)

2 protocols using fitc conjugated a 629511

Investigating Pancreatic Macrophages in Smoke Exposure

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Immunofluorescence analysis of the pancreas after smoke exposure was performed on 5-μm-thick tissue sections mounted onto glass slides that were baked overnight at 58°C. Tissues were deparaffinized with xylene, rehydrated in decreasing concentrations of ethanol and permeabilized for 30 min with methanol solution. Antigen retrieval was performed by boiling sections in 0.5% citrate buffer for 15 min, blocked with 2.5% horse serum for 1 h and incubated with murine F4/80 and Arginase1 (Santa Cruz Biotechnology, Santa Cruz, CA) primary antibodies overnight at 4°C. Slides were washed with PBS and incubated with FITC-conjugated (A-629511, Invitrogen, Grand Island, NY, USA) and Alexa-Fluor-647 (A-21447, Invitrogen)-conjugated secondary antibodies for 1 h, washed three times and mounted with Vectashield containing 4′ 6′-diamidino-2-phenylindole (DAPI).

Edderkaoui M., Xu S., Chheda C., Morvaridi S., Hu R.W., Grippo P.J., Mascariñas E., Principe D.R., Knudsen B., Xue J., Habtezion A., Uyeminami D., Pinkerton K.E, & Pandol S.J. (2016). HDAC3 mediates smoking-induced pancreatic cancer. Oncotarget, 7(7), 7747-7760.

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Immunofluorescence Analysis of Smoke-Exposed Pancreas

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Immunofluorescence analysis of pancreas after smoke exposure was performed on 5-μm-thick tissue sections mounted onto glass slides that were baked overnight at 58 °C. Tissues were deparaffinized with xylene, rehydrated in decreasing concentrations of ethanol and permeabilized for 30 min with methanol solution. Antigen retrieval was performed by boiling sections in 0.5% citrate buffer for 15 min, blocked with 2.5% horse serum for 1 h and incubated with murine F4/80 and HB-EGF (sc1414, Santa Cruz Biotechnology) primary antibodies overnight at 4 °C. Slides were washed with PBS and incubated with FITC-conjugated (A-629511, Invitrogen, Grand Island, NY, USA) and Alexa-Fluor-647 (A-21447, Invitrogen)-conjugated secondary antibodies for 1 h, washed three times and mounted with Vectashield containing 4′ 6′-diamidino-2-phenylindole (DAPI).

Kumar S., Torres M., Kaur S., Rachagani S., Joshi S., Johansson S., Momi N., Baine M., Gilling C., Smith L., Wyatt T., Jain M., Joshi S, & Batra S. (2014). Smoking accelerates pancreatic cancer progression by promoting differentiation of MDSCs and inducing HB-EGF expression in macrophages. Oncogene, 34(16), 2052-2060.

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