MCF-7, Madin Darby Canine Kidney (MDCK), and L cells from the American Type Culture Collection (ATCC) were cultured under established conditions [29 (link)]. Wnt3a-secreting L cells and HCT116 cells were provided by Dr. Min DS and Dr. Kim YJ (Pusan National University, Pusan, Korea), respectively. The Gln deprivation experiment was performed by applying EMEM without Gln (GIBCO, Carlsbad, CA, USA). Recombinant TGF-β (R&D Systems, MN, USA) was applied to cells at a concentration of 10 ng/ml. 40 μM DON (Sigma, St. Louis, MO, USA), 5 μM 968 (Calbiochem, San Diego, CA, USA), 10 μM BPTES (Sigma), or 1 mM dimethyl-α-ketoglutarate (α-KG; Sigma) were also applied to cells.
Dimethyl α ketoglutarate α kg
Manufactured by Merck Group
Sourced in United States
Dimethyl-α-ketoglutarate (α-KG) is a chemical compound that serves as a core metabolic intermediate. It is a dicarboxylic acid derivative of the amino acid glutamic acid. α-KG plays a central role in the citric acid cycle, a fundamental metabolic pathway in living organisms.
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Lab products found in correlation
2 protocols using dimethyl α ketoglutarate α kg
1
Cell Culture and Metabolic Modulation
2
Wound Healing Assay for Cell Migration
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Migratory ability was assessed by a wound healing assay as described previously (Zhao et al, 2012 (link)). Cells were seeded into a 6-well plate and allowed to grow to 90–100% confluence in DMEM, and the cell monolayers were then wounded by a 200 μl pipette tip. The wounded monolayers were washed four times with phosphate-buffered saline and incubated in serum-deprived DMEM with or without dimethyl-α-ketoglutarate (α-KG; Sigma Aldrich) for the indicated times (Csibi et al, 2013 (link)). Distances covered by migrated cells were quantified.
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