Nb 100 110

Manufactured by Merck Group

The NB-100-110 is a compact and versatile laboratory equipment designed for general-purpose applications. It features a durable construction and various standard functionalities to support common laboratory tasks. The core function of the NB-100-110 is to provide a reliable and practical solution for routine laboratory operations.

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Lab products found in correlation

H3k4me1, by Merck Group (1 mentions) H3k4me3, by Cell Signaling Technology (1 mentions) H3k27ac, by Abcam (1 mentions) Normal rabbit igg, by Merck Group (1 mentions) Cay10006421, by Novus Biologicals (1 mentions)

2 protocols using nb 100 110

Chromatin Immunoprecipitation Profiling of Epigenetic Factors

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Chromatin immunoprecipitation was performed as previously described 7 using antibodies against HIF‐1 (PM14), HIF‐2 (PM9) 42, 43, HIF‐1 (Novus NB‐100‐110), H3K4me1 (Millipore, #07‐436), H3K4me3 (#9751, Cell Signaling Technology), H3K27ac (#ab4729, Abcam), CTCF (#07‐729, Millipore), and RNApol2 (#sc‐899, Santa Cruz Biotechnology). Each ChIP was performed in duplicate. Libraries were prepared using PrepX Complete ILMN DNA library kit (#400076, Wafergen).

Platt J.L., Salama R., Smythies J., Choudhry H., Davies J.O., Hughes J.R., Ratcliffe P.J, & Mole D.R. (2016). Capture‐C reveals preformed chromatin interactions between HIF‐binding sites and distant promoters. EMBO Reports, 17(10), 1410-1421.

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ChIP-seq protocol for HIF-1α/2α and histone marks

Cited 2 times

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Chromatin immunoprecipitation (ChIP) experiments were performed as previously described [7 (link),43 (link)–45 (link)] using antibodies directed against HIF-1α (rabbit polyclonal, PM14 or Cayman Chemicals, Cay10006421), HIF-2α (rabbit polyclonal, PM9 or PM8), HIF-1β (rabbit polyclonal, Novus Biologicals, NB100-110), H3K4me1 (rabbit polyclonal, Millipore, #07–436), H3K4me3 (rabbit monoclonal, Cell Signaling Technology, #9751) or H3K27ac (rabbit polyclonal, Abcam, #ab4729). Non-immunized rabbit serum or purified normal rabbit IgG (Millipore, #12–370) were used as negative controls as appropriate.

Grampp S., Schmid V., Salama R., Lauer V., Kranz F., Platt J.L., Smythies J., Choudhry H., Goppelt-Struebe M., Ratcliffe P.J., Mole D.R, & Schödel J. (2017). Multiple renal cancer susceptibility polymorphisms modulate the HIF pathway. PLoS Genetics, 13(7), e1006872.

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