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8 protocols using potassium persulfate

1

Synthesis and Characterization of Ti3AlC2 Composite Materials

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Ti3AlC2 was purchased from American Elements (USA). LiF was purchased from Waco (Japan). HCl, NaCl, CaCl2·2H2O, AA, sodium metabisulfite (SM), potassium persulfate (PP), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) were purchased from Daejung Co., Ltd (Korea). TEOS and APTES were purchased from Tokyo Chemical Industry Co., Ltd (Japan). Acetic acid and MB were purchased from Samchun Pure Chemical Co., Ltd (Korea). AMPS, DMAPS, and α-Al2O3 were purchased from Sigma Aldrich (USA). Lithium chloride (LiCl) and MV were purchased from Junsei Chemical Co., Ltd (Japan). Lithium hydroxide (LiOH) monohydrate was purchased from Duksan Co., Ltd (Korea).
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2

Antioxidant Evaluation of Pumpkin Leaves

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Pumpkin (Cucurbita moschata Duch.) leaves were purchased from a local market, and were washed, dried, ground (−30 mesh), and stored in a refrigerator at 4°C until needed. DPPH and 2,4,6-tripyridyl-S-triazine (TPTZ) were purchased from Sigma Chemical Co. (St. Louis, MO, USA) and from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), respectively. Potassium persulfate, ferric chloride hexahydrate, and ferrous sulfate heptahydrate were purchased from Daejung Chemicals, Ltd. (Gyeonggi, Korea), Junsei Chemical Co., Ltd. (Tokyo, Japan), and Wako Pure Chemicals Industries, Ltd. (Osaka, Japan), respectively. N,O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) was purchased from Supelco (Bellefonte, PA, USA). All phenolic standards were purchased from Fluka (Steinheim, Switzerland).
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3

Dendropanax morbifera Leaf-Hyaluronate Hydrogel

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The leaves of six-year-old Dendropanax morbifera Léveille and ginsenoside Rh2 (≥90%) were acquired from Ginseng Bank, Kyung Hee University (Yongin, Republic of Korea). High-molecular-weight sodium hyaluronate (HA, MW = 1,280 kDa) was supplied by Xinjiang Fufeng Biotechnologies Co., Ltd. (Xinjiang, China). Zinc nitrate hexahydrate (reagent grade, ≥98%), N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC, reagent grade, ≥98%), 4-(dimethylamino) pyridine (DMAP, ≥99%), hydrochloric acid (HCl, ACS reagent, 37%), and anhydrous N,N-dimethylformamide (DMF, ≥99%) were purchased from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Phosphate buffer (20× PBS) was purchased from Biosesang (Seongnam, Republic of Korea). Trichloroacetic acid (TCA, ≥ 99%), potassium persulfate (≥98%), potassium ferricyanide (≥98%), sodium chloride (NaCl, ≥99.50%), and sodium hydroxide (NaOH, ≥98%) were purchased from Daejung Chemicals and Metals Co., Ltd. (Siheung, Republic of Korea). Glycerol was purchased from Junsei Chemical Co., Ltd (Tokyo, Japan). Iron (III) chloride hexahydrate (97%), methyl alcohol (MeOH, 95%), and ethyl alcohol (EtOH, 95%) were procured from Samchun Pure Chemical Co. Ltd. (Pyeongtaek, Republic of Korea). Other chemicals and reagents were purchased from commercial suppliers.
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4

Fermentation of Lactobacillus reuterii AKT1

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All chemical reagents were of analytical grade. Acetonitrile, ethanol, acetone, methanol, sodium carbonate, sodium hydroxide, anhydrous sodium acetate, hydrochloric acid, potassium persulfate, acetic acid and sulfuric acid were purchased from Daejung Chemicals and Metals Co., Ltd., South Korea. The phenolic standards and other chemicals like Folin–Ciocalteu reagent, Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl); ABTS (2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), TPTZ (2,4,6-Tris(2-pyridyl)-s-triazine) and gallic, ferulic, caffeic, o-Coumaric and p-Coumaric acids were obtained from Sigma, South Korea.
Lactobacillus reuterii AKT1 and all other strains used in our study were obtained from the Department of Food Science and Biotechnology, Kangwon National University, Korea, to be used for fermentation. The bacteria stock culture was stored at −80 °C, in MRS broth (Difco), containing 20% glycerol (v/v).
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5

Antioxidant Activity Quantification Protocol

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Ethanol, acetonitrile, acetone, mEthanol, phosphate-buffered saline (PBS), sodium carbonate, penicillin-streptomycin, sodium hydroxide, anhydrous sodium acetate, hydrochloric acid, trypsin EDTA, Williams’ Medium E, potassium persulfate, acetic acid, sulfuric acid, gentamicin, insulin, L-glutamine, Dulbecco’s modified eagle medium (DMEM) and advanced DMEM were obtained from Daejung chemicals and metals Co., Ltd, South Korea and Life Technologies (Grand Island, NY, USA). The Phenolic standards and other chemicals like ABTS (2,2′Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), 2′,7′-Dichlorofluorescin diacetate or 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA, ≥ 97% purity), TPTZ (2,4,6-Tris(2-pyridyl)-s-triazine), caffeic acid, ferulic acid, 2,2 Diphenyl-1-picrylhydrazyl (DPPH), gallic acid, Folin–Ciocalteu reagent, quercetin dehydrate (≥ 98% purity), p-Coumaric acid, (+)-catechin hydrate, dimethyl sulfoxide (DMSO); 2,2′-azobis(2-amidinopropane) dihydrochloride (ABAP), and Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were purchased from Sigma, South Korea. Analytical grade chemicals and reagents were used.
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6

Antioxidant Properties of Garlic-Based Food

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Garlic powder was purchased from HeungSeong Food Co. Ltd. (Hongcheon, Korea). Fish fillet was provided from DongKwang Food Co. (Busan, Korea). Salt (CJ CheilJedang Co., Sinan, Korea), Sugar (CJ CheilJedang Co., Incheon, Korea), Wheat Flour (Daehan Flour Co., Incheon, Korea), Cooking oil (Sajodaerim Co., Incheon, Korea) were purchased from a local supermarket. The experimental reagents 2,2-diphenyl-1-picrylhydrazyl, 2 N Folin & Ciocalteu reagent, gallic acid, quercetin, and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) were purchased from Sigma–Aldrich Co. (St. Louis, MO, USA). Ethyl alcohol, sodium carbonate, sodium hydroxide, and potassium persulfate, among others, were purchased from Daejung Chemicals & Metals Co. (Siheung, Korea). All reagents and solvents were of Grade 1 quality.
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7

Synthesis of Organic Compounds and Nanomaterials

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Sodium hydride 60%, magnesium turnings, and potassium persulfate were purchased from Daejung, S. Korea. Dimethylformamide (DMF), 4-vinylbenzyl chloride (VBC), 2-acrylamido-2-methylpropane sulfonic acid (AMPS), 4,4′-azobis(4-cyanovaleric acid) (ABCA), dimethyl sulfoxide (DMSO), butyl methacrylate (BMA), bromobenzene, and iodine were purchased from Sigma-Aldrich, Schnelldorf, Germany. Pyrroles and carbon disulfide were purchased from Daejung, Korea, diethyl ether from Sigma-Aldrich, Schnelldorf, Germany, magnesium sulfate and petroleum ether were from Fisher Scientific, Chicago, IL, USA, and tetrahydrofuran (THF) was purchased from Daejung, Korea. Silica-gel, FeCl2.4H2O, and FeCl3.6H2O were purchased from UniChem USA. n-Hexane was purchased from Sigma-Aldrich, Germany. Aprepitant and sofosbuvir were donated by Ferozsons pharmaceutical company, Nowshera, Pakistan.
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8

Antioxidant activity evaluation protocol

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All chemicals and reagents used were of analytical grade. Ethanol, methanol, iron(III) chloride, aluminium chloride, potassium persulfate, sodium nitrite, iron(II) sulfate, acetic acid, sodium acetate trihydrate, ethyl acetate and hydrochloric acid were from Dae-Jung (Gyeonggi, Korea). Folin-Ciocalteu phenol reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), sodium hydroxide, ferrozine, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), ethylenediaminetetraacetic acid (EDTA), catechin and gallic acid were from Sigma-Aldrich, Merck (Darmstadt, Germany).
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