Citral (CAS: 5392-40-5), cuminaldehyde (CAS: 122-03-2), and perillaldehyde (CAS: 6611-91-2) were obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) at purities of at least 98. 0%, 97. 0%, and 87. 0%, respectively, as determined by gas chromatography (GC). Citronellal (CAS: 106-23-0), cinnamaldehyde (CAS: 101-39-3), hydroxycitronellal (CAS: 107-75-5), and vanillin (CAS: 121-33-5) were obtained from Wako Pure Chemical Industries Ltd. (Osaka, Japan) at purities of at least 85%, 98%, and 98%, respectively, as determined by GC, and vanillin was obtained at a purity of at least 98% as determined by thin layer chromatography (TLC) titration.
Vanillin
Manufactured by Fujifilm
Sourced in Japan
Vanillin is a laboratory chemical used as a reagent in various analytical and research applications. It is a white crystalline powder with a characteristic vanilla aroma. Vanillin is commonly used as a standard in spectrophotometric and chromatographic techniques to quantify the presence and concentration of certain organic compounds. Its core function is to serve as a reference material in these analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Laboran screw tube jar no 7, by As One (3 mentions)
Vanillic acid, by Fujifilm (3 mentions)
Veratraldehyde, by Fujifilm (2 mentions)
Protocatechualdehyde, by Fujifilm (2 mentions)
Citral, by Tokyo Chemical Industry (1 mentions)
Cinnamaldehyde, by Fujifilm (1 mentions)
Tryptone, by BD (1 mentions)
Dimethyl sulfoxide (dmso), by Kanto Chemical (1 mentions)
Methanol, by Fujifilm (1 mentions)
Yeast extract, by Nacalai Tesque (1 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions)
Cycloheximide (chx), by Nacalai Tesque (1 mentions)
Furfural, by Fujifilm (1 mentions)
Tsk gel ods 100s, by Tosoh (1 mentions)
Sodium hydroxide (naoh), by Fujifilm (1 mentions)
Veratryl alcohol, by Fujifilm (1 mentions)
P hydroxybenzaldehyde, by Fujifilm (1 mentions)
Ferulic acid, by Fujifilm (1 mentions)
Salicylaldehyde, by Fujifilm (1 mentions)
Benzaldehyde, by Fujifilm (1 mentions)
12 protocols using vanillin
1
Purification and Characterization of Aromatic Aldehydes
2
Preparing Powder Composition with Increased Void Volume
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Example 6
Into a LABORAN screw tube jar No. 7 (manufactured by AS ONE Corporation), 9.5 g of the powderizing agent A and 0.5 g of Vanillin (manufactured by Wako Pure Chemical Industries, Ltd.) were placed. These were kept at 60° C. for 0.5 hours and completely melt for mixing. Here, the amount of the liquid component used was 5% by mass relative to the total mass of the powder composition (powderizing agent A+liquid component). Next, the mixture was cooled in a 10° C.-thermostatic chamber for 0.5 hours (tempering process) and then was allowed to stand in a 20° C.-thermostatic chamber for 12 hours. Thus, a powder composition having voids with an increased volume was obtained.
The characteristics of the obtained powder composition were as follows.
loose bulk density: 0.3 g/cm3
average particle diameter: 140 μm
expansion ratio: ×2.9
As illustrated in
Examples 7 to 9 employed an orange dye (hydrophobic dye) as the liquid component.
3
Powder Composition with Increased Volume
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Example 4
Into a LABORAN screw tube jar No. 7 (manufactured by AS ONE Corporation), 9.9 g of the powderizing agent A and 0.1 g of Vanillin (manufactured by Wako Pure Chemical Industries, Ltd.) were placed. These were kept at 60° C. for 0.5 hours and completely melt for mixing. Here, the amount of the liquid component used was 1% by mass relative to the total mass of the powder composition (powderizing agent A+liquid component). Next, the mixture was cooled in a 10° C.-thermostatic chamber for 0.5 hours (tempering process) and then was allowed to stand in a 20° C.-thermostatic chamber for 12 hours. Thus, a powder composition having voids with an increased volume was obtained.
The characteristics of the obtained powder composition were as follows.
loose bulk density: 0.3 g/cm3
average particle diameter: 116 μm
expansion ratio: ×4.0
As illustrated in
4
Analytical Techniques for Phenolic Compounds
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Ferulate, p-coumarate, caffeate, protocatechuate, syringate, cinnamate, vanillate, vanillin, ethyl acetate, and methanol (HPLC and LC-MS/MS grades) were purchased from Wako Pure Chemical Industries Ltd., (Tokyo, Japan). DMSO was bought from Kanto Chemical Co., Inc. (Tokyo, Japan). Yeast extract and agar were purchased from Nacalai Tesque Inc. (Kyoto, Japan). Tryptone was purchased from Becton, Dickinson and Company (Tokyo, Japan).
5
Olfactory Function Evaluation via Vanillin
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A two-bottle preference test using vanillin was conducted as a behavioral test to evaluate olfactory function [31 (link)]. The 0.1% vanillin solution did not induce a gustatory response and the rodents tended to dislike the smell of vanillin. Therefore, an increase in the consumption rate of 0.1% aqueous vanillin solution would imply a decrease in olfactory function [32 ]. Bottles containing only distilled water and bottles containing vanillin-water (0.1% vanillin [FUJIFILM Wako Pure Chemical Corporation] dissolved in distilled water) were placed in the cages, and bottle positions were changed daily. Bottle content intake was measured every other week, and avoidance was calculated by dividing normal water intake by the sum of normal water intake and vanillin-water intake. An avoidance ratio close to 100% indicates healthy olfactory function, whereas an avoidance ratio close to 50% indicates that the animals drank the two waters equally and were unable to distinguish the smell of vanillin. In other words, the rats were in a state of olfactory dysfunction.
6
Expanded Powder Composition with Vanillin
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Example 5
Into a LABORAN screw tube jar No. 7 (manufactured by AS ONE Corporation), 9.7 g of the powderizing agent A and 0.3 g of Vanillin (manufactured by Wako Pure Chemical Industries, Ltd.) were placed. These were kept at 60° C. for 0.5 hours and completely melt for mixing. Here, the amount of the liquid component used was 3% by mass relative to the total mass of the powder composition (powderizing agent A+liquid component). Next, the mixture was cooled in a 10° C.-thermostatic chamber for 0.5 hours (tempering process) and then was allowed to stand in a 20° C.-thermostatic chamber for 12 hours. Thus, a powder composition having voids with an increased volume was obtained.
The characteristics of the obtained powder composition were as follows.
loose bulk density: 0.3 g/cm3
average particle diameter: 139 μm
expansion ratio: ×2.7
As illustrated in
7
Vanillin Quantification and Microscopy
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Vanillin, furfural, and dimethyl sulfoxide (DMSO) were obtained from Wako (Osaka, Japan). Cycloheximide (CHX) was purchased from Nacalai Tesque, Kyoto, Japan. Stock solutions of 2 M Vanillin, 2 M furfural, and 20 mg/ml CHX were prepared in DMSO and stored at -30°C. Vanillin levels in SD medium were assayed by the methods of Fitzgerald et al. (2003) (link). Briefly, after spinning down cells, the supernatant was separated using a linear gradient elution of 10–45% acetonitrile in 0.1% trifluoroacetic acid by high performance liquid chromatography (HPLC; Shimadzu, Kyoto, Japan) equipped with a C18 reversed-phase column, TSKgel ODS-100S (4.6 mm × 150 mm; Tosoh, Tokyo, Japan). The column was maintained at 40°C. The eluted compounds were detected at 280 nm. A Leica AF6500 (Leica Microsystems Vertrieb GmbH, Germany) fluorescence microscopic system was used for the microscopic analysis. Cells treated with Vanillin were immediately observed without fixation. All experiments were repeated at least three times.
8
Organosolv Lignin Model Compounds
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1.5 mol L−1 Bu4NOH aq. was purchased from the Sigma-Aldrich Co. The solution was diluted to 1.25 mol L−1 with distilled water and used as reaction solution. Vanillin, vanillic acid, veratraldehyde, veratric acid, veratryl alcohol, and sodium hydroxide were purchased from FUJIFILM Wako Pure Chemical Co. The particle size of Japanese cedar wood flour was 90–180 μm. Lignin model compounds, veratryl glycerol-β-guaiacyl ether (LM1) and guaiacyl glycerol-β-guaiacyl ether (LM2), were purchased from Tokyo-Kasei Co. and used without purification (see Scheme 1 for the structures of LM1 and LM2).
9
Enzymatic Assays for Aromatic Aldehydes
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Vanillin (4-hydroxy-3-methoxybenzaldehyde), vanillic acid (4-hydroxy-3-methoxybenzoic acid), ferulic acid (4-hydroxy-3-methoxycinnamic acid), isoVanillin (3-hydroxy-4-methoxybenzaldehyde), protocatechualdehyde (3,4-dihydroxybenzaldehyde), benzaldehyde, p-hydroxybenzaldehyde, m-anisaldehyde (3-methoxybenzaldehyde), veratraldehyde (3,4-dimethoxybenzaldehyde), syringaldehyde (4-hydroxy-3,5-dimethoxybenzaldehyde), and salicylaldehyde (2-hydroxybenzaldehyde) were purchased from Wako Pure Chemicals (Osaka, Japan). NAD(P)+ and lactate dehydrogenase from the rabbit muscle were purchased from Oriental Yeast Co. Ltd. (Osaka, Japan). Oligonucleotides were purchased from Life Technologies Japan Ltd. (Tokyo, Japan).
10
Inhibiting Yeast Growth with Natural Compounds
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Vanillin, vanillic acid, 3.4-dihydroxy-5-methoxybenzaldehyde (Wako Pure Chemical Industries, Osaka, Japan), 2-methoxyhydroquinone (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), protocatechuic aldehyde (Sigma-Aldrich, St. Louis, MO, USA), and protocatechuic aldehyde (Nacalai Tesque, Kyoto, Japan) were purchased and used as inhibitors of yeast growth in cultures. Cellulase (mainly containing endo-β-1,4-glucanase and β-1,4-glucosidase) from A. niger [54 (link), 55 (link)] was obtained from Tokyo Chemical Industry Co., Ltd., and its activity was 29,500 unit/g. Rice straw was grown and harvested on the farm at Meijo University (Aichi, Japan). The straw was cut, dried at 45 °C for 3 h, and milled to a particle size of 1 mm, followed by washing at a weight ratio of 1:20 of rice straw to distilled deionized water. The washed straw was dried at 45 °C for 24 h and used for subsequent experiments.
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