β zearalanol β zal

Methanol and acetonitrile, both HPLC grade, were obtained from Merck (Darmstadt, Germany). Formic acid and ammonium acetate were obtained from Sigma-Aldrich (St. Louis, MO, USA). Water was purified using a Milli-Q Plus apparatus (18.2 Ω, Millipore, Billerica, MA, USA) prior to use. The standards of aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), aflatoxin M1 (AFM1), aflatoxin M2 (AFM2), OTA, ochratoxin α (OTα), fumonisin B1 (FB1), T-2 toxin (T-2), HT-2 toxin (HT-2), deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), fusarenon X (FUS-X), ZEN, α-zearalenol (α-ZEL), β-zearalenol (β-ZEL), α-zearalanol (α-ZAL), and β-zearalanol (β-ZAL) were purchased from Romer Labs (Union, MO, USA). The mycotoxin conjugates deoxynivalenol-3-glucuronide (DON-3-GlcA) and zearalenone-14-glucuronide (ZEN-14-GlcA) were prepared in our laboratory as described in a previous study [12 (link)]. The quantification of DON-15-GlcA was based on the standard concentration curve of DON-3-GlcA as described in a previous study [21 (link)].

AFB1, AFB2, AFG1, AFG2, AFM1, AFM2, ochratoxin A (OTA), ochratoxin α (OTα), deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), neosolaniol (NEO), fusarenon X (Fus X), citrinin (CIT), fumonisins B1 (FB1), fumonisins B2 (FB2), zearalenone (ZEN), zearalanone(ZAN), α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), α-zearalanol (α-ZAL), and β-zearalanol (β-ZAL) were purchased from Romer Labs, Inc. (Union, MO, USA). Sterigmatocystin (STM), gliotoxin (GLI), cyclopiazonic acid (CPA), patulin (PAT), alternariol (AOH), alternariolmethyether (AME) and altenuisin (ALS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile, hexane, acetone, formic acid, chloroauric acid, cysteine and ammonium acetate were also obtained from Sigma-Aldrich. Milli-Q water (Millipore, Billerica, MA, USA) was used for all analyses.
A total of 40 samples, including 20 peanut samples, 10 wheat samples, and 10 maize samples, were purchased from bazaars in Shanghai, China.