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Ls174t

Manufactured by Korean Cell Line Bank
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LS174T is a human colorectal adenocarcinoma cell line. It is a widely used in vitro model for studying colorectal cancer biology and related research applications.

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Lab products found in correlation

Hct116, by Korean Cell Line Bank (10 mentions) Ht 29, by Korean Cell Line Bank (7 mentions) Dld 1, by Korean Cell Line Bank (6 mentions) Sw480, by Korean Cell Line Bank (6 mentions) Sw620, by Korean Cell Line Bank (5 mentions) Colo205, by Korean Cell Line Bank (4 mentions) Hct 15, by Korean Cell Line Bank (3 mentions) Sw1116, by Korean Cell Line Bank (3 mentions) Km12c, by Korean Cell Line Bank (3 mentions) Km12sm, by Korean Cell Line Bank (3 mentions) Snu c1, by Korean Cell Line Bank (2 mentions) Mg132 m7449, by Merck Group (2 mentions) Cycloheximide chx c4859, by Merck Group (2 mentions) Bix01294 ab141407, by Abcam (2 mentions) Colo 320, by Korean Cell Line Bank (2 mentions) Sw403, by Korean Cell Line Bank (2 mentions) Colo201, by Korean Cell Line Bank (2 mentions) Snu c5, by Korean Cell Line Bank (2 mentions) Km12l4, by Korean Cell Line Bank (2 mentions) Mda mb 231, by American Type Culture Collection (1 mentions)

10 protocols using ls174t

1

Cell Culture and Inhibitor Treatments

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MCF-7, MDA-MB-231 and HEK 293T/17 cells (American Type Culture Collection, Manassas, VA, USA), and Colo205, Lovo, SW480, SW620, DLD-1, HCT116, HT29, LS174T and SNUC1 cells (Korean Cell Line Bank, Seoul, Republic of Korea) were cultured in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich Corp., St. Louis, MO, USA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (Sigma-Aldrich Corp.) and 1% (v/v) penicillin/streptomycin solution (Lonza, Basel, Switzerland) at 37 °C in a 5% CO2 incubator. In some experiments, cells were treated with specific inhibitors (Calbiochem, San Diego, CA, USA) of GSK3β (SB415286 or SB216763), p38MAPK (SB203580) or CatB (CA-074 or cathepsin inhibitor III).
Oh S.S., Park S., Lee K.W., Madhi H., Park S.G., Lee H.G., Cho Y.Y., Yoo J, & Dong Kim K. (2017). Extracellular cystatin SN and cathepsin B prevent cellular senescence by inhibiting abnormal glycogen accumulation. Cell Death & Disease, 8(4), e2729-.
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2

Colorectal Cancer Cell Line Cultivation

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The human CRC cell lines HCT116 and LS174T were purchased from the Korean Cell Line Bank and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin in a humidified atmosphere with 5% CO2 at 37 °C. Sodium propionate (SP; P5436), sodium butyrate (SB; 303410), sodium acetate (SA; 71183), and sodium D-lactate (SL; 71716) were purchased from Sigma-Aldrich. MG132 (M7449) and cycloheximide (CHX; C4859) were purchased from Sigma-Aldrich. BIX01294 (ab141407) was purchased from Abcam.
Ryu T.Y., Kim K., Han T.S., Lee M.O., Lee J., Choi J., Jung K.B., Jeong E.J., An D.M., Jung C.R., Lim J.H., Jung J., Park K., Lee M.S., Kim M.Y., Oh S.J., Hur K., Hamamoto R., Park D.S., Kim D.S., Son M.Y, & Cho H.S. (2022). Human gut-microbiome-derived propionate coordinates proteasomal degradation via HECTD2 upregulation to target EHMT2 in colorectal cancer. The ISME Journal, 16(5), 1205-1221.
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3

Culturing and Treating CRC Cell Lines

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The human CRC cell lines, HCT116, SW480, SW620 and LS174T, were purchased from the Korean Cell Line Bank (KCLB nos. #10247, #10228, #10227 and #10188, respectively), and cultured in RPMI-1640 medium, supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) at 37°C in a 5% CO2 incubator. HT29 cells (KCLB no. #30038), which originate from AN adenocarcinoma of the rectosigmoid part of the intestine (30 (link)), were cultured in RPMI-1640 medium supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.). KM12SM cells (KCLB no. #80016) were cultured in DMEM supplemented with 10% FBS. For STAT3 inhibitor treatment, the cells were treated with stattic (5 µM; Selleck Chemicals LLC). For EDNRA inhibitor treatment, the CRC cells were treated with macitentan (2 or 10 µM; Merck KGaA).
Lee Y.J., Jung E., Choi J., Hwang J.S., Jeong E.J., Roh Y., Ban H.S., Kim S., Kim S.K., Kim S.Y., Min J.K., Han T.S, & Kim J.S. (2022). The EDN1/EDNRA/β-arrestin axis promotes colorectal cancer progression by regulating STAT3 phosphorylation. International Journal of Oncology, 62(1), 13.
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4

Culturing Human CRC Cell Lines

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Human CRC cell lines (colo-201, colo-205, colo-320, HCT-15, HCT-116, HT-29, Lovo, LS174T, SNU-61, SNU-70, SNU-81, SNU-175, SNU-254, SNU-283, SNU-407, SNU-977, SNU-1033, SNU-1181, SNU-1235, SNU-1411, SNU-1544, SNU-1684, SNU-C1, SNU-C2A, SNU-C4, SNU-C5, SW-403, and SW-480) were purchased from the Korean Cell Line Bank [18 (link)]. All cell lines were cultured in RPMI1640 (Hyclone Laboratories, Ind., Logan, UT), supplemented with 10% fetal bovine serum, at 37°C in humidified 5% CO2.
Jang J.E., Kim H.P., Han S.W., Jang H., Lee S.H., Song S.H., Bang D, & Kim T.Y. (2018). NFATC3–PLA2G15 Fusion Transcript Identified by RNA Sequencing Promotes Tumor Invasion and Proliferation in Colorectal Cancer Cell Lines. Cancer Research and Treatment : Official Journal of Korean Cancer Association, 51(1), 391-401.
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5

Comprehensive CRC Cell Line Characterization

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Forty-eight human CRC cell lines (SNU-61, SNU-70, SNU-81, SNU-175, SNU-254, SNU-283, SNU-407, SNU-479, SNU-503, SNU-769A, SNU-769B, SNU-796, SNU-977, SNU-1033, SNU-1040, SNU-1047, SNU-1181, SNU-1197A, SNU-1235, SNU-1411, SNU-1406, SNU-1544, SNU-1684, SNU-1746, SNU-C1, SNU-C2A, SNU-C4, SNU-C5, Caco-2, COLO201, COLO205, COLO320, DLD1, HCT-8, HCT-15, HCT-116, HT-29, LoVo, LS174T, NCI-H716, SW-403, SW-480, SW-620, SW-1116, WiDr, KM12C, KM12SM, and KM12L4) were obtained from the Korean Cell Line Bank. All cell lines were maintained in RPMI1640 media with 10% fetal bovine serum at 37°C and 5% CO2. All cell lines were tested and authenticated by Korean Cell Line Bank using DNA finger printing analysis.
Kim S.C., Hong C.W., Jang S.G., Kim Y.A., Yoo B.C., Shin Y.K., Jeong S.Y., Ku J.L, & Park J.G. (2018). Establishment and Characterization of Paired Primary and Peritoneal Seeding Human Colorectal Cancer Cell Lines: Identification of Genes That Mediate Metastatic Potential. Translational Oncology, 11(5), 1232-1243.
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6

Cell Lines Used in Colorectal Cancer Research

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Human CRC cell lines including HCT116, HT29, SW480, DLD1, LoVo, and LS174T were purchased from the Korean Cell Line Bank (Seoul, Republic of Korea). Human colon epithelial cell line FHC and human embryonic kidney cell line HEK293T were purchased from the American Type Culture Collection (Rockville, MD, USA). Murine colon adenocarcinoma cell line MC-38 was purchased from Kerafast (Boston, MA, USA). Patient-derived colorectal cancer cells (hCRC#1 and hCRC#2, Table S1) were acquired and cultured as described in previous reports 18 (link). All cells were cultured in accordance with the supplier's instructions. All experiments were performed within 20 passages from the first thaw, and cells were routinely tested for mycoplasma contamination using the e-MycoTM mycoplasma detection kit (iNtron Biotechnology, Seongnam, Republic of Korea).
Kim J.H., Park S.Y., Jeon S.E., Choi J.H., Lee C.J., Jang T.Y., Yun H.J., Lee Y., Kim P., Cho S.H., Lee J.S, & Nam J.S. (2022). DCLK1 promotes colorectal cancer stemness and aggressiveness via the XRCC5/COX2 axis. Theranostics, 12(12), 5258-5271.
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7

Culturing Colorectal Cancer Cell Lines

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The colorectal cancer cell lines HCT-116, SW1116, LS174T, HT-29, KM12C, KM12SM, SW48, SW480, SW620, DLD-1, LOVO, COLO205 were obtained from the Korean Cell Line Bank (Seoul, Korea) and were maintained in DMEM (Gibco-BRL, Grand Island, NY) supplemented with 10% fetal bovine serum and 100 μg/ml antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin. Cells were maintained at 37°C in a humidified, 5% CO2/air atmosphere.
Park S.Y., Lee S.J., Cho H.J., Kim T.W., Kim J.T., Kim J.W., Lee C.H., Kim B.Y., Yeom Y.I., Lim J.S., Lee Y, & Lee H.G. (2016). Dehydropeptidase 1 promotes metastasis through regulation of E-cadherin expression in colon cancer. Oncotarget, 7(8), 9501-9512.
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8

Cultivation of Human CRC Cell Lines

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Human CRC cell lines, including HCT‐116, WiDr, HT‐29, SNU‐C5, DLD‐1, HCT‐15, and LS174T, were obtained from the Korean Cell Line Bank (Seoul, Korea).24 Cells were cultured in RPMI 1640 media containing 10% heat‐inactivated fetal bovine serum (Gibco, Carlsbad, CA) and gentamicin (10 μg/mL) at 37 °C in a 5% CO2 atmosphere.
Park Y., Kim H., Cho Y., Min D., Cheon S., Lim Y.J., Song S., Kim S.J., Han S., Park K.J, & Kim T. (2018). Activation of WNT/β‐catenin signaling results in resistance to a dual PI3K/mTOR inhibitor in colorectal cancer cells harboring PIK3CA mutations. International Journal of Cancer, 144(2), 389-401.
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9

Colorectal Cancer Cell Line Cultivation

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Twelve CRC cell lines (DLD1, HT29, SW620, HCT116, LoVo, KM12C, KM12SM, KM12L4, HCT116, LS174T, SW1116, and Colo205) were purchased from the Korean Cell Line Bank (https://cellbank.snu.ac.kr, Seoul, Korea). Cells were cultured in RPMI1640, MEM, DMEM, or L15 medium (Welgene, Daegu, Korea) at 37 °C in a humidified incubator with 5% CO2 according to the guidelines. All culture media contained 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA) and 1% penicillin/streptomycin (Gibco).
Jang B.G., Kim H.S., Bae J.M., Kim W.H., Kim H.U, & Kang G.H. (2020). SMOC2, an intestinal stem cell marker, is an independent prognostic marker associated with better survival in colorectal cancers. Scientific Reports, 10, 14591.
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10

CRC Cell Line Culture and Treatments

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The human CRC cell lines HCT116 and LS174T were purchased from the Korean Cell Line Bank and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin in a humidi ed atmosphere with 5% CO 2 at 37˚C. Sodium propionate (SP; P5436) was purchased from Sigma-Aldrich. MG132 (M7449) and cycloheximide (CHX; C4859) were purchased from Sigma-Aldrich. BIX01294 (ab141407) was purchased from Abcam.
Ryu T.Y., Kim K., Han T., Lee M., Lee J., Choi J., Jeong E., An D.M., Jung C., Jung J., Park K., Park D., Kim D., Son M., & Cho H. (2020). Histone methyltransferase EHMT2 degradation by propionate derived from Bacteroides thetaiotaomicron induced colon cancer apoptosis via epigenetic regulation of TNFAIP1.
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