For cell-surface staining, aliquots of 106 splenic mononuclear cells were incubated with fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD4 (GK1.5) antibody (eBioscience, San Diego, CA, USA). For intracellular staining, cells stained with CD4 were incubated with fixation/permeabilization working solution, and Fc receptors were blocked with excess mouse Fc block. Cells were then stained with anti-phycoerythrin (PE)-Cy5-conjugated anti-mouse Foxp3 (FJK-16s) and anti-allophycocyanin (APC)-CD25 antibodies (eBioscience). CD4+CD25+Foxp3+ T cells were analyzed by flow cytometry (FACSCalibur; Becton Dickinson, San Jose, CA, USA).15 (link),16 (link)
Fitc conjugated anti mouse cd4 gk1.5 antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FITC)-conjugated anti-mouse CD4 (GK1.5) antibody is a fluorescently labeled monoclonal antibody that specifically recognizes the mouse CD4 cell surface antigen. This antibody can be used for the identification and enumeration of CD4+ T cells in flow cytometry applications.
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2 protocols using fitc conjugated anti mouse cd4 gk1.5 antibody
1
Identification of Regulatory T Cells
2
Identification of Regulatory T Cells
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For cell-surface staining, aliquots of 10 6 splenic mononuclear cells were incubated with fluorescein isothiocyanate (FITC)conjugated anti-mouse CD4 (GK1.5) antibody (eBioscience, San Diego, CA, USA). For intracellular staining, cells stained with CD4 were incubated with fixation/permeabilization working solution, and Fc receptors were blocked with excess mouse Fc block. Cells were then stained with anti-phycoerythrin (PE)-Cy5-conjugated anti-mouse Foxp3 (FJK-16s) and anti-allophycocyanin (APC)-CD25 antibodies (eBioscience). CD4 + CD25 + Foxp3 + T cells were analyzed by flow cytometry (FACSCalibur; Becton Dickinson, San Jose, CA, USA) (12, 13) .
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