WT mice (C57BL/6) for the drug screen and EPO injection assays were purchased from The Jackson Laboratory. CD45.1+ WT mice for BM transplantation were purchased from Charles River Laboratories. Jak2V617F floxed (Jak2VF/+) hematopoietic-specific knockin mice (Jak2VF/+Vav-Cre) (originally reported in ref. 42 (link)), Plek2-KO, and Jak2VF/+Vav-Cre, Plek2-KO mice were previously reported (12 (link)). Pten floxed (Ptenfl/fl) mice were purchased from The Jackson Laboratory. These mice were crossed with Mx-Cre mice (The Jackson Laboratory) to generate hematopoietic-specific Pten-KO mice. These mice were further crossed with Plek2-KO mice to generate Ptenfl/flMx-Cre, Plek2-KO mice. To test the CBCs, peripheral blood (75 μL from each mouse) was collected from retroorbital veins in EDTA-treated minicollection tubes (Greiner Bio-One GmbH). The Hemavet 950 complete blood counter (Drew Scientific) was used to determine CBCs. For pathologic analyses, the leg bones, spleen, and lungs were collected from euthanized mice and treated with formalin solution (MilliporeSigma) before H&E staining.
Mx cre mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
Mx-Cre mice are a type of genetically modified mouse strain developed for use in research. They express the Cre recombinase enzyme under the control of the Mx1 promoter, which is induced by type I interferons. This allows for the targeted deletion or modification of specific genes in cells that express the Mx1 gene, such as hematopoietic cells.
Automatically generated - may contain errors
Lab products found in correlation
Vav cre mice, by Jackson ImmunoResearch (2 mentions)
Wt c57bl 6 mice, by Jackson ImmunoResearch (2 mentions)
Cd45.1 wt mice, by Charles River Laboratories (1 mentions)
Formalin solution, by Merck Group (1 mentions)
R26 yfp mice, by Jackson ImmunoResearch (1 mentions)
Cag cas9 transgenic mice, by Jackson ImmunoResearch (1 mentions)
Mmtv cre, by Jackson ImmunoResearch (1 mentions)
Hotstartaq dna polymerase, by Qiagen (1 mentions)
6 protocols using mx cre mice
1
Transgenic Mice for Hematological Studies
2
Characterization of Transgenic Mouse Strains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
G9afl mice (Lehnertz et al. 2010 (link)), Vav-Cre mice (Stadtfeld and Graf 2005 (link)), and R26-YFP mice (Ye et al. 2003 (link)) were described earlier, and Mx-Cre mice were obtained from Jackson Laboratory. All strains were maintained on a pure C57/B6 background and used between 6 and 12 wk of age. All procedures were conform with institutional guidelines.
3
Induction of Fbxo9 cKO and Cbfb-MYH11 Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fbxo9 cKO mice were developed using Easi-CRISPR, as previously published [29 (link)], and bred with Cbfb+/56M [29 (link),32 (link)] or Mx-cre mice, purchased from Jackson Laboratories (#003556, Bar Harbor, ME, USA). PCR confirmed the genotype and the expression of these genes (primers listed in Supplemental Materials and Methods). To induce cKO of Fbxo9 and expression of Cbfb-MYH11, 6–8 week-old floxed mice and littermate controls received three intraperitoneal Poly(I:C) injections every other day, at a dose of 10 µg per gram body weight (Invivogen, San Diego, CA, USA). Procedures performed were approved by the Institutional Animal Care and Use Committee of the University of Nebraska Medical Center in accordance with NIH guidelines (protocol number: 15-099-11-FC).
4
Genetically Modified Mice for Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Genetically modified tissue-specific mDia2 knockout mice with a C57/BL6 background were described previously25 (link),26 (link). Congenic mice carrying CD45.1 antigen were purchased from Charles River (B6-LY-5.2/Cr, strain code: 564). C57/BL6 WT mice, CAG-Cas9 transgenic mice (stock #026179), Mx-Cre mice (stock #003556), and Vav-Cre mice (stock #008610) were purchased from the Jackson Laboratory. All the experiments involving animals were conducted in accordance with the Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee at Northwestern University.
5
Generation and Characterization of RANK Knockout Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 1
Mice.
Rankfloxed mice have been recently generated1. Briefly, to generate mice carrying a null allele of Rank (rankΔ allele), rankfloxed mice were crossed to β-actin-Cre ubiquitous deleter mice. Mice carrying the rankfloxed or rankΔ alleles as well as the MMTV-Cre mice were backcrossed seven times onto a BALBc background before generating the MMTV-Cre rankΔ/floxed mice. MMTV-NeuT mice were kindly provided by Guido Forni, Milan. MMTV-Cre (stock #003553) and Mx-Cre mice (stock #003556) were obtained from the Jackson Laboratory. K5-Cre, IKKαfloxed and NFATc1floxed mice have been previously described2-4. Mouse geno-types were determined by PCR and Southern blot analysis. In all experiments, only littermate mice from the same breedings were used. All mice were bred and maintained according to institutional guidelines.
RANK Deletion in Tumors and Cre Effects.
Southern blotting of the tumors that developed in RANKΔmam females showed deletion of RANK, albeit some residual wild type band was observed (
6
Genetically Engineered Mice Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were housed at Massachusetts General Hospital in a specific pathogen–free environment. All animal experiments were performed after approval and according to the guidelines and supervision of the Massachusetts General Hospital Subcommittee on Research Animal Care. B6.SJL (ref: 002014) recipient mice and Mx-Cre mice were purchased from The Jackson Laboratory. The R26-M2rtTA::TetOP-Cre mice (TetO-Cre) will be available from The Jackson Laboratory (JAX Stock# 021025). All animals were genotyped by Southern blot or by PCR using the HotStarTaq DNA polymerase (QIAGEN).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!