β1-AR, β2-AR, β3-AR (Santa Cruz Biotechnology, Santa Cruz, CA), Gαs, Gαi, and GAPDH (Cell Signaling Technology, Danvers, MA) protein expression was determined via Western Blot analysis. Thirty micrograms of left and right ventricular homogenates were resolved on SDS-PAGE gradient gels (4–15%, Tris-Glycine) and transferred to polyvinylidine difluoride membranes. Primary antibody incubation occurred at 4°C for a minimum of 16 hours. Blots were then washed with 0.1% TBST, followed by incubation with anti-HRP secondary antibodies (Cell Signaling) for a minimum of 1 hour at room temperature. Band intensities were visualized using chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate, Thermo Scientific, Rockford, IL, USA) and quantified using NIH ImageJ Data Acquisition Software (National Institutes of Health, Bethesda, MD, USA).
β1 ar
Manufactured by Santa Cruz Biotechnology
Sourced in United States
β1-AR is a lab equipment product used in scientific research. It functions as a receptor for the neurotransmitter norepinephrine, which plays a role in various physiological processes.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Thermo Fisher Scientific (3 mentions)
β2 ar, by Santa Cruz Biotechnology (2 mentions)
β3 ar, by Santa Cruz Biotechnology (2 mentions)
Pierce ecl western blotting substrate, by Thermo Fisher Scientific (2 mentions)
Peroxidase conjugated goat anti rabbit igg, by Jackson ImmunoResearch (2 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)
Calreticulin, by Cell Signaling Technology (1 mentions)
Calmodulin, by Cell Signaling Technology (1 mentions)
P erk1 2 t202 y204, by Cell Signaling Technology (1 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
Phospho creb, by Cell Signaling Technology (1 mentions)
Catalase, by Merck Group (1 mentions)
Il 1β, by Santa Cruz Biotechnology (1 mentions)
Nlrp3, by Merck Group (1 mentions)
Mnsod, by Merck Group (1 mentions)
Hotstar taq, by Takara Bio (1 mentions)
Asc 1, by Santa Cruz Biotechnology (1 mentions)
P enos ser1177, by Cell Signaling Technology (1 mentions)
Reverse transcription system kit, by Promega (1 mentions)
4 protocols using β1 ar
1
Cardiac Protein Expression Analysis
2
Cardiac Signaling Protein Assay
3
Western Blot Analysis of Cell Signaling
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The expressions of β1-AR(Santa Cruz Biotechnology), β2-AR(Santa Cruz Biotechnology), phospho-CREB, CREB(Cell Signaling Technology, Danvers, Massachusetts, USA) and HuR(Santa Cruz Biotechnology) were examined by western-blot. All cell samples were lysed in lysis buffer. The protein concentration was assessed by BCA protein assay kit (Life Technologies). Proteins were subjected to electrophoresis with 10% SDS polyacrylamide gel and transferred to NC membranes. The membranes were analyzed with antibodies according to the supplier’s protocol, and immunolabelled bands were visualized by use of the Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific, San Jose, CA, USA).
4
Nebivolol Modulates Autophagy and eNOS
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Anti-autophagy-related protein 5 (ATG5) and phospho-endothelial nitric oxide synthase (p-eNOS, Ser1177) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against BNP, PGC-1α, IL-1β, β1-AR, β2-AR, β3-AR and ASC-1 were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against ATG7, LC3B, Actin and NLRP3 were from Sigma (St. Louis, MO, USA). Anti-4-Hydroxy-2-noneal Michael Adducts (4-HNE), Caspase-1, MnSOD and catalase were from Millipore (Billerica, MA, USA). Peroxidase-conjugated rabbit anti-goat IgG, peroxidase-conjugated rabbit anti-mouse IgG, and peroxidase-conjugated goat anti-rabbit IgG were from Jackson ImmunoResearch (West Grove, PA, USA). The BCA™ protein assay kit and Pierce ECL Western blotting substrate were from Thermo Scientific (Rockford, IL, USA). Nebivolol was provided by Hanxiang Company (Wuhan, China). The reverse transcription system kit was from Promega (Mannheim, Germany) and HotStarTaq from Takara (Otsu, Shiga, Japan). D-loop, Nrf1, Tfam and 18S rRNA primers were synthesised by Bioasia Biotech (Shanghai, China). Lentiviral shRNA β3-AR and shRNA against eNOS constructs were synthesised by Genechem Corporation (Shanghai, China).
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