Plasma or serum samples from subjects at the same time points before and after vaccination were measured for soluble CD14 (sCD14), CXCL-13 using standard ELISA assays according to manufacturer’s instructions (Quantikine® ELISA, R & D Systems, Minneapolis, MN) and for the following chemokines and cytokines: IL-2, IL-4, IL-6, IL-10, IL-12p70, IL-15, IL-17A, IL-21, TNF-α, and IFN-γ using the Luminex xMap platform multiplex assay (EMD Millipore, Billerica, MA). TNF-α and IL-6 levels were furthered confirmed by standard ELISA kits (R&D). Quantitative assessment of hepatitis B surface antibody titer was performed using the MONOLISA™ assay (BioRad, Hercules, CA) according manufacturer instructions.
Luminex xmap platform multiplex assay
Manufactured by Merck Group
The Luminex xMAP platform is a multiplex assay technology that allows for the simultaneous detection and quantification of multiple analytes in a single sample. It utilizes color-coded magnetic microspheres or beads, each coated with a specific capture molecule, to perform multiple assays in a high-throughput manner. This platform enables the efficient and accurate measurement of a wide range of analytes, including proteins, peptides, nucleic acids, and small molecules, in a variety of sample types.
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Lab products found in correlation
2 protocols using luminex xmap platform multiplex assay
1
Immunological Biomarker Assessment Post-Vaccination
2
Vaccination Immunobiomarker Profiling
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Plasma or serum samples from subjects at the same time points before and after vaccination were measured for soluble CD14 (sCD14), CXCL-13 using standard ELISA assays according to manufacturer's instructions (Quantikine® ELISA, R & D Systems, Minneapolis, MN) and for the following chemokines and cytokines: IL-2, IL-4, IL-6, IL-10, IL-12p70, IL-15, IL-17A, IL-21, TNF-α, and IFN-γ using the Luminex xMap platform multiplex assay (EMD Millipore, Billerica, MA). TNF-α and IL-6 levels were furthered confirmed by standard ELISA kits (R&D). Quantitative assessment of hepatitis B surface antibody titer was performed using the MONOLISA™ assay (BioRad, Hercules, CA) according manufacturer instructions.
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