Stainings were performed on sections from brain tissues fixed in formalin and treated with concentrated formic acid to inactivate prions. Partially protease-resistant prion protein deposits, astrogliosis and microglia deposition were visualized by staining brain sections with the SAF84 antibody (1:200, SPI bio), GFAP (1:1000, Millipore) and IBA1 (1:2500, WAKO) respectively on a NexES immunohistochemistry robot (Ventana instruments) using an IVIEW DAB Detection Kit (Ventana), after preceding incubation with protease 1 (Ventana). Images of DAB stained sections were acquired using the NanoZoomer scanner (Hamamatsu Photonics) and NanoZoomer digital pathology software (NDPview; Hamamatsu Photonics). Quantifications of IBA1, GFAP staining and vacuoles in mouse sections were performed on acquired images; regions of interest were drawn on a Digital Image Hub (Leica Biosystems) and analyzed as previously described [77 ].
Nanozoomer digital pathology software ndpview
Manufactured by Hamamatsu Photonics
The NanoZoomer digital pathology software (NDPview) is a product designed for digital pathology analysis. It provides a platform to view and manage digital slide images captured by the NanoZoomer series of whole slide scanners. The software allows users to access, navigate, and analyze high-resolution digital pathology samples.
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Lab products found in correlation
Iview dab detection kit, by Roche (2 mentions)
Nanozoomer scanner, by Hamamatsu Photonics (2 mentions)
Protease 1, by Roche (1 mentions)
Digital image hub, by Leica (1 mentions)
Glial fibrillary acidic protein (gfap), by Merck Group (1 mentions)
Bx61 upright fluorescent microscope, by Olympus (1 mentions)
Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions)
2 protocols using nanozoomer digital pathology software ndpview
1
Immunohistochemical Analysis of Prion Protein
2
Histological Analysis of Prion Pathology
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Brain tissues were fixed in formalin and treated with concentrated formic acid to inactivate prions. About 2µm thick sections were prepared from these brains and deparaffinized using graded alcohols and then subjected to antigen retrieval using 10mM citrate buffer (pH 6). Astrogliosis, microgliosis, and the presence of protease‐resistant prion deposits were visualized by staining brain sections with GFAP (1∶1000, Agilent technologies), IBA1 (1∶2500, WAKO), and the SAF84 antibody (1∶200, SPI bio), respectively on a NexES immunohistochemistry robot (Ventana instruments) using an IVIEW DAB Detection Kit (Ventana). Sections were also counterstained with hematoxylin and eosin when appropriate. Images were acquired using NanoZoomer scanner (Hamamatsu Photonics) and visualized using NanoZoomer digital pathology software (NDPview; Hamamatsu Photonics). Images were acquired using Olympus BX61 Upright fluorescent microscope. Quantifications of IBA1, GFAP staining was performed on the entire brain section from the acquired images using Image J.
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