Standard methods were used for yeast culture, transformation, and manipulation (Amberg et al, 2005 ). WT BY4741 (MATa, his3Δ1, leu2Δ0, met15Δ0, and ura3Δ0) and PAB1-TAP (MATa, leu2Δ0, met15Δ0, ura3Δ0, and PAB1-TAP::His3Mx6) strains were purchased from Dharmacon. GAPDH-ProtA (MATa, leu2Δ0, met15Δ0, ura3Δ0, and TDH3-ProtA:: His3Mx6) strain was generated in this study. A fragment of the TAP tag comprising the tobacco etch virus (TEV) cleavage target sequence, the two protein A tandem sequences, the ADH1 terminator, and the His3MX6 selection marker were amplified by PCR. Sequences complementary to the end of the TDH3 gene were included by a second PCR, and the corresponding cassette was used to transform a WT strain for genomic integration of the TEV-Protein A cassette in frame with the TDH3 gene.
Wt by4741
Manufactured by Horizon Discovery
WT BY4741 is a wild-type yeast strain commonly used in laboratory research. It serves as a reference strain for various genetic and molecular studies involving the budding yeast Saccharomyces cerevisiae.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using wt by4741
1
Yeast Strain Construction and Modification
2
Yeast Strain Generation and Tagging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Standard methods were used for yeast culture, transformation, and manipulation (Amberg et al, 2005 ). WT BY4741 (MATa, his3Δ1, leu2Δ0, met15Δ0, and ura3Δ0) and PAB1-TAP (MATa, leu2Δ0, met15Δ0, ura3Δ0, and PAB1-TAP::His3Mx6) strains were purchased from Dharmacon. GAPDH-ProtA (MATa, leu2Δ0, met15Δ0, ura3Δ0, and TDH3-ProtA::His3Mx6) strain was generated in this study. A fragment of the TAP tag comprising the tobacco etch virus (TEV) cleavage target sequence, the two protein A tandem sequences, the ADH1 terminator, and the His3MX6 selection marker were amplified by PCR. Sequences complementary to the end of the TDH3 gene were included by a second PCR, and the corresponding cassette was used to transform a WT strain for genomic integration of the TEV-Protein A cassette in frame with the TDH3 gene.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!