The largest database of trusted experimental protocols

Bromocresolgreen

Manufactured by Fujifilm
Sourced in Japan

Bromocresolgreen is a chemical compound used as a pH indicator in various laboratory applications. It is a solid, crystalline substance that changes color based on the pH of the solution. Bromocresolgreen can be utilized to determine the acidity or basicity of a sample within a specific pH range.

Automatically generated - may contain errors

2 protocols using bromocresolgreen

1

Fabrication of Dye-Based Colorimetric Paper

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bromophenol blue, methyl red, bromocresol
green, disperse orange
3, bromocresol purple, fluorescein, and cresol red were purchased
from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). EA, DET,
and TMA were purchased from Alfa Aesar Co. (Great Britain). All other
reagents were of analytical grade and used as received. For the fabrication
of the DCP, Whatman chromatography paper grade 1 (200 × 200 mm,
pure cellulose paper) was obtained from GE Healthcare (China). The
designed DCPs were printed on a sheet of Whatman grade #1 using a
wax printer (ColoQube 8570DN, Xerox, USA) followed by heating at 120
°C for 2 min on a drying machine. Wax ink was used for the paper
hydrophobization and an insulation agent in this work.
The DCP
consisted of circles with an inside diameter of 5 mm and a border
of 2 mm (after melting wax ink). To prepare the DCP, the circles were
filled with 10 μL of 0.10 mM dye solutions and dried for 1 h
at room temperature. The colors of the DCP were recorded with a smartphone.
The spots on DCP with different dyes were assigned as shown in Figure 7.
+ Open protocol
+ Expand
2

Quantitative Analysis of Protein-Acrolein Adducts

Check if the same lab product or an alternative is used in the 5 most similar protocols
The abundance of protein-acrolein adducts in lung homogenates and plasma was analyzed by Western blot as described before (Conklin et al., 2009 (link)). Western blots of lung homogenates were also probed for albumin using mouse albumin antibody (1:10,000, Bethyl Laboratories)(Cho, 2002, 53). Western blots were developed using ECL plus reagent (Pierce, Thermo Scientific), and band intensities were detected with an myECL imager (Thermo Scientific). Quantification of band intensity was performed using myImage analysis software (Thermo Scientific). Amido black protein stain was used as loading control (protein) where indicated. Total plasma cholesterol (Cholesterol CII Enzymatic Kit, Wako), triglycerides (L-Type TG-H Kit, Wako), protein (Bradford, Wako), albumin (bromocresol green, Wako), alanine aminotransferase (ALT, Infiniti), aspartate aminotransferase (AST, Infiniti), creatine kinase (CK, Promega) and lactate dehydrogenase (LDH, Promega) levels were measured using commercially available assay reagents and calibrated standards on a Cobas Mira Plus 5600 Autoanalyzer (Conklin et al., 2009 (link)).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!