90 plus

Manufactured by Brookhaven Instruments

Sourced in United States

The 90 Plus is a particle size analyzer that measures the size distribution of particles in a sample. It uses dynamic light scattering technology to determine the hydrodynamic diameter of particles ranging from 0.6 nanometers to 6 micrometers.

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Lab products found in correlation

Jem 2100, by JEOL (4 mentions) Nicolet 6700, by Thermo Fisher Scientific (3 mentions) Axio observer z1, by Zeiss (3 mentions) Drx nmr spectrometer, by Bruker (2 mentions) F 4600, by Hitachi (2 mentions) Uv 3600, by Shimadzu (2 mentions) Uv 2450, by Shimadzu (2 mentions) Carbon type b, by Ted Pella (2 mentions) Escalab 250xi, by Thermo Fisher Scientific (2 mentions) Archimedes, by Malvern Panalytical (2 mentions) Ultra 55, by Zeiss (2 mentions) Lsm 800, by Zeiss (1 mentions) Polyethylenimine (pei), by Merck Group (1 mentions) Arx 400 nmr spectrometer, by Bruker (1 mentions) Model uv 1700 spectrometer, by Shimadzu (1 mentions) Jem 2010 transmission electron microscope, by JEOL (1 mentions) Zetasizer nano zs90, by Malvern Panalytical (1 mentions) Ls55 spectrofluorometer, by PerkinElmer (1 mentions) Particle solutions software, by Brookhaven Instruments (1 mentions) Infinite 200, by Tecan (1 mentions)

Close spelling variants of this product

90 Plus Particle Size Analyzer (103 citations) BI-90 Plus particle size analyzer (7 citations) Zeta PALS BI-90 Plus (7 citations) 90 Plus particle sizer (6 citations) Brookhaven 90-PLUS instrument (5 citations) BIC 90 plus (4 citations) Zeta PALS/90 plus (4 citations) Brookhaven 90 plus Particle Analyzer (4 citations) Model 90 plus (3 citations) 90 Plus Particle Analyzer (3 citations)

81 protocols using 90 plus

Characterization and Modeling of CTS-nZVI

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The surface morphology and structure of CTS-nZVI were observed using a scanning electron microscope (FE-SEM, JEOL JSM-7001M, Tokyo, Japan). The binding energy of CTS-nZVI was recorded by X-ray photoelectron spectroscopy (Thermo Fisher ESCALAB 250Xi, Waltham, MA, USA). Functional groups of CTS-nZVI were analyzed by a Fourier transform infrared spectrometer (Shimadzu IRAffinity-1S, Tokyo, Japan). The settling rate of CTS-nZVI in solution and the concentration of iron were quantified by UV–Vis spectroscopy (UNICO 2082S UV/VIS, Shanghai, China). The average size and size distribution of CTS-nZVI were measured by the dynamic light scattering technique (Brookhaven Instruments Corporation 90 PLUS, New York, NY, USA). Zeta potential of CTS-nZVI was determined using a Brookhaven 90 PLUS zeta potential analyzer (Brookhaven Instruments Corporation 90 PLUS, New York, NY, USA). The pH was measured using a pH-meter (Mettler Toledo MP 220, Zurich, Switzerland).
In this study, the settling curves of different pH and ionic strengths were fitted by Origin 2018 software. The standard deviation calculation of the parallel test and the diagram in this paper were plotted using Origin 2018 software. The numerical model of water flow and solute transport in porous media was established by using software version 4.0 of HYDRUS-1D.

Huang D., Ren Z., Li X, & Jing Q. (2021). Mechanism of Stability and Transport of Chitosan-Stabilized Nano Zero-Valent Iron in Saturated Porous Media. International Journal of Environmental Research and Public Health, 18(10), 5115.

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Particle Characterization of Formulations

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The mean particle size and polydispersity index of the formulations were measured by dynamic light scattering (90 Plus, Brookhaven Instruments Corporation, Holtsville, NY, USA). The zeta potential of the formulations was determined using a zeta potential analyzer (90 Plus, Brookhaven Instruments Corporation). The dispersion of the prepared formulations was diluted tenfold with triply deionized water for measurement of both size and surface charge. Particle characterizations were repeated three times per sample for three independent batches.

Chang L.W., Hou M.L., Hung S.H., Lin L.C, & Tsai T.H. (2015). Pharmacokinetics of quercetin-loaded nanodroplets with ultrasound activation and their use for bioimaging. International Journal of Nanomedicine, 10, 3031-3042.

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Characterizing Nanoparticle Formulations

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The different NSs formulations were further characterized in vitro to evaluate their size and surface charge. The average diameter and polydispersity index of the formulations were measured using photon correlation spectroscopy (PCS) with a 90 Plus instrument (Brookhaven, NY, USA) at a fixed angle of 90 and a temperature of 25 °C after dilution with filtered water. The zeta potential was determined using a 90 Plus instrument (Brookhaven, NY, USA). For zeta-potential determination, diluted samples of nanoformulations were placed in an electrophoretic cell, to which a rounded 15 V/cm electric field was applied. The pH was recorded at room temperature using an Orion 420A pH meter.

Tannous M., Hoti G., Trotta F., Cavalli R., Higashiyama T., Pagliaro P, & Penna C. (2023). Oxygen Nanocarriers for Improving Cardioplegic Solution Performance: Physico-Chemical Characterization. International Journal of Molecular Sciences, 24(12), 10073.

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PEI-Coated Extracellular Vesicle Characterization

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The EVs were engineered by coating EVs with branched polyethylenimine (PEI, MW 25,000 Sigma-Aldrich) by using a non-covalent layer-by-layer protocol (Angelini et al., 2007 (link), 2008 (link)). The EVs pellet (100 μL), dispersed with 2 mL of PBS, was added of 2 mL of PEI dissolved in 0.3 M NaCl (final concentration of PEI 0.05 mg/mL) and incubated for 20 min at room temperature. The concentration was chosen because demonstrated to be the best compromise between activity and toxicity (Diomede et al., 2018a (link)).
The obtained suspension was centrifuged at 4000 rpm for 15 min, the supernatant was removed in order to get rid of excess PEI and the precipitate was resuspended in 2 mL of PBS. The EVs suspension was characterized by using dynamic light scattering (DLS) experiments (Brookhaven Instruments Corporation, 90Plus, Holtsville, New York, United States).
To evaluate the interaction between EVs or PEI-EVs and hPDLSCs, WGA Alexa Fluor 488 stained EVs and PEI-EVs were analyzed at confocal laser scanning microscope (CLSM) (LSM800; Zeiss, Jena, Germany) level after 24 h of incubation.

Pizzicannella J., Gugliandolo A., Orsini T., Fontana A., Ventrella A., Mazzon E., Bramanti P., Diomede F, & Trubiani O. (2019). Engineered Extracellular Vesicles From Human Periodontal-Ligament Stem Cells Increase VEGF/VEGFR2 Expression During Bone Regeneration. Frontiers in Physiology, 10, 512.

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Comprehensive Characterization of Nanoparticles

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NMR spectra were performed on a Bruker ARX 400 NMR spectrometer. Electrospray ionization mass spectrometry (ESI-MS) was performed on a Proteome X-LTQ. Particle size and size distribution were determined by laser light scattering (LLS) with a particle size analyzer (90 Plus, Brookhaven Instruments Co., United States) at a fixed angle of 90° at room temperature. The zeta potential was determined by a Malvern Zetasizer Nano ZS90 (Worcestershire, UK). TEM images were obtained from a JEOL JEM-2010 transmission electron microscope with an accelerating voltage of 200 KV. UV-vis absorption spectra were taken on a Shimadzu Model UV-1700 spectrometer. Photoluminescence (PL) spectra were measured on a Perkin-Elmer LS 55 spectrofluorometer. All UV and PL spectra were collected at 24 ± 1 °C.

Xu S., Yuan Y., Cai X., Zhang C.J., Hu F., Liang J., Zhang G., Zhang D, & Liu B. (2015). Tuning the singlet-triplet energy gap: a unique approach to efficient photosensitizers with aggregation-induced emission (AIE) characteristics †Electronic supplementary information (ESI) available: Synthesis and characterization of the intermediates and molecular orbital data. See DOI: 10.1039/c5sc01733e Click here for additional data file.. Chemical Science, 6(10), 5824-5830.

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Liposome Size Characterization by DLS

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The size distribution of the liposomes was measured by dynamic light scattering (DLS) using a Brookhaven 90Plus particle analyzer running Particle Solutions Software (Version 2.6, Brookhaven Instruments Corporation) with a 659 nm laser and a 90° detection angle. Each measurement was performed at a count rate between 200 and 500 kilocounts/s for 2 min. The scattering signal at the position of the detector fluctuates due to the diffusion of liposomes in the solution. The instrument directly measures the diffusion constant D of the liposomes by fitting the cross-correlation function of the time signal measured by the detector. This is related to the particle size via the Stokes-Einstein relation:

D = \frac{k_{B} T}{6 π η r}

, where η is the dynamic viscosity of the solution, k_B is the Boltzmann constant, T is the sample temperature and r is the radius of the LUVs, assumed to be spherical. All measurements were performed at 25°C on 1 ml of sample containing ≈20 mg/ml of RBC liposomes.

Himbert S., D’Alessandro A., Qadri S.M., Majcher M.J., Hoare T., Sheffield W.P., Nagao M., Nagle J.F, & Rheinstädter M.C. (2022). The bending rigidity of the red blood cell cytoplasmic membrane. PLoS ONE, 17(8), e0269619.

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Functionalization of Mesoporous Silica Nanoparticles

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NH₂-MSNs were suspended at 10 mg mL⁻¹ and pH was adjusted to near neutral (7.0–7.5). 2 kDa NHS-PEG-FITC (NANOCS) was dissolved in DMSO and added to the particle suspension at ratios varying from 2 : 1 to 200 : 1 (wt/wt). The suspension was reacted on a shaker for 2 h at room temperature. Once the reaction was complete, the reaction supernatant was collected, and absorption was read at 494 nm (Tecan Infinite 200) to measure the amount of PEG-FITC reacted to the surface of the MSN. For flow cytometry experiments, 2 kDa mPEG-NHS succinic acid (NANOCS) was used in place of FITC-PEG-NHS. The zeta potential of each particle formulation was measured (90Plus, Brookhaven Instruments) to ensure a positive surface charge had been retained.

Becicka W.M., Bielecki P.A., Lorkowski M.E., Moon T.J., Zhang Y., Atukorale P.U., Covarrubias G, & Karathanasis E. (2021). The effect of PEGylation on the efficacy and uptake of an immunostimulatory nanoparticle in the tumor immune microenvironment. Nanoscale Advances, 3(17), 4961-4972.

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Lipid-Coated Fluorescent Silica Beads

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Reverse phase evaporation was used to coat green fluorescent silica beads (Discovery Scientific, USA) with a lipid bilayer (inner leaflet: DOTAP; outer leaflet: 85 mol% PtC, 10% pHrodo-Red (P36600, Life Technologies) conjugated to DPPE, and 5% DSPE-PEG2k). The bead size distribution, concentration, and zeta potential were measured with qNano (Izon, Oxford, UK) and dynamic light scattering (90 Plus, Brookhaven). The final product had an average diameter of 530 nm.

Vo H., Chiu J., Allaimo D., Mao C., Wang Y., Gong Y., Ow H., Porter T, & Zhong X. (2014). High fat diet deviates PtC-specific B1 B cell phagocytosis in obese mice. Immunity, Inflammation and Disease, 2(4), 254-261.

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Zeta Potential Characterization of NPs

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The zeta potential (surface charge) of each NP sample formed at various N:P ratios was determined at 25°C with a scattering angle of 90° using a potential measurement analyzer (90PLus; Brookhaven Instruments Corporation, Holtsville, NY, USA). Samples were prepared in PBS and diluted with deionized water to ensure that the measurements were performed under conditions of low ionic strength, where the surface charge of the particles can be measured accurately.

Zhu Y., Liang G., Sun B., Tian T., Hu F, & Xiao Z. (2016). A novel type of self-assembled nanoparticles as targeted gene carriers: an application for plasmid DNA and antimicroRNA oligonucleotide delivery. International Journal of Nanomedicine, 11, 399-411.

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Synthesis of PEG-Lipoic Acid Coated AuNPs

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Bare 15 nm AuNPs were prepared by citrate reduction: To 12 L of water, 60 mL of 2% tetrachloroauric acid (HAuCl₄) were added and the solution boiled. 360 mL of 1% sodium citrate (w/v) were then added and the solution boiled for 30 min. After cooling, 6 mL of 4N K₂CO₃ were added, adjusting the pH to 8.5. 1.8 mL of 1% SH-PEG2k-OCH₃ (dissolved in water) and 3.6 mL of 1% lipoic acid (dissolved in ethanol) were combined in 15mL of water. The PEG/Lipoic acid mixture was added to the Au solution. After 1 hour, the AuNPs were purified and concentrated to 100 mg Au/mL using a Minimate TFF system (Pall, Inc., Port Washington, NY, USA) overnight, then transferred to a 15 mL 100K membrane filter (Millipore, Billerica, MA, USA) to purify and concentrate further. 500 mg Au in Au-lipoic acid/PEG particles was obtained; gold yield = 83%. Gold core size was measured by transmission electron microscopy (FEI BioTwin G2 Transmission Electron Microscope, Hillsboro, OR, USA) and solution hydrodynamic diameter measured by dynamic light scattering (Brookhaven Instruments Corp. 90 Plus, Holtsville, NY, USA).

Hainfeld J.F., Lin L., Slatkin D.N., Dilmanian F.A., Vadas T.M, & Smilowitz H.M. (2014). Gold Nanoparticle Hyperthermia Reduces Radiotherapy Dose. Nanomedicine : nanotechnology, biology, and medicine, 10(8), 1609-1617.

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