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Ctglur1

Manufactured by Thermo Fisher Scientific

CtGluR1 is a laboratory equipment product from Thermo Fisher Scientific. It is designed to measure the activity of the glutamate receptor 1 (GluR1) protein, which is involved in synaptic transmission and neuronal function. The CtGluR1 device provides a quantitative assessment of GluR1 activity levels in experimental samples.

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2 protocols using ctglur1

1

Immunohistochemical Analysis of Neuronal Markers

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All animals were perfused and collected brain tissues as same as mentioned above. Brain sections at 30 μm thickness were sliced for immunostaining studies, primary antibodies against ctGluR1(Rabbit, Invitrogen, PA5-99527), c-Fos (rabbit, Cell Signaling Technology, #2250), NR1(rabbit, ABclonal, A7677), VMAT2 (rabbit, Phoenix Pharmaceuticals, H-V008), or Mc4R (rabbit, Alomone labs, Cat#AMR-024). All brain sections were incubated with the primary antibodies overnight at room temperature following 1 h blocking in 10% normal donkey serum. After visualized with secondary donkey IgG serum conjugated with Alexa Fluor 488 or Alexa Fluor 647 (Jackson ImmunoResearch, West Grove, PA, USA), sections were photographed with a TCS SP5 confocal microscopy.
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2

Immunohistochemical Analysis of Neuronal Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
All animals were perfused and collected brain tissues as same as mentioned above. Brain sections at 30 μm thickness were sliced for immunostaining studies, primary antibodies against ctGluR1(Rabbit, Invitrogen, PA5-99527), c-Fos (rabbit, Cell Signaling Technology, #2250), NR1(rabbit, ABclonal, A7677), VMAT2 (rabbit, Phoenix Pharmaceuticals, H-V008), or Mc4R (rabbit, Alomone labs, Cat#AMR-024). All brain sections were incubated with the primary antibodies overnight at room temperature following 1 h blocking in 10% normal donkey serum. After visualized with secondary donkey IgG serum conjugated with Alexa Fluor 488 or Alexa Fluor 647 (Jackson ImmunoResearch, West Grove, PA, USA), sections were photographed with a TCS SP5 confocal microscopy.
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