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Alexa594 conjugated anti goat igg

Manufactured by Thermo Fisher Scientific

Alexa Fluor 594-conjugated anti-goat IgG is a secondary antibody designed for use in various immunodetection techniques. It is specifically reactive with goat immunoglobulin G (IgG) and is labeled with the Alexa Fluor 594 fluorescent dye, which exhibits red fluorescence when excited at the appropriate wavelength.

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2 protocols using alexa594 conjugated anti goat igg

1

Immunohistochemical Analysis of IL-17 in Mouse Aortic Sinus

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Mouse aortic sinus tissue slides were fixed in 4% paraformaldehyde and were incubated with goat anti-IL-17 (sc-6077, Santa Cruz Biotechnology) or isotype control. The slides were further incubated with Alexa594-conjugated anti-goat IgG (Invitrogen) then were mounted with mounting medium containing DAPI (Vector). The slides were examined by using a Zeiss Axio Observer.D1m fluorescence microscopy with DAPI or Texas Red filters.
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2

Fluorescent Detection of IL-17 in Mouse Aortic Tissue

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Mouse aortic sinus tissue slides were fixed in 4% paraformaldehyde and were incubated with goat anti-IL-17 (sc-6077; Santa Cruz Biotechnology, Dallas, TX, USA) or isotype control. The slides were further incubated with Alexa594-conjugated anti-goat IgG (Invitrogen) and were mounted with mounting medium containing DAPI (vector). The slides were examined with a Zeiss Axio Observer.D1M fluorescence microscope with DAPI or Texas Red filters. The intensity was determined using NIH ImageJ software.
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