Gold mag mini whole blood genomic dna purification kit

We selected five SNPs from previously reported RTEL1 gene polymorphisms, and matched SNPs with MAF > 5% in the HapMap Asian population selected for association analysis [16 , 27 (link), 28 (link)]. Venous blood samples (5 mL) were collected from each Participant during a laboratory examination. DNA was extracted from whole blood samples using the Gold Mag-Mini Whole Blood Genomic DNA Purification Kit (version 3.0; TaKaRa, Japan) [29 (link)]. The DNA concentration was measured by spectrometry (DU530 UV/VIS spectrophotometer, Beckman Instruments, Fullerton, CA, USA). The Sequenom MassARRAY Assay Design 3.0 software (Sequenom, Inc, San Diego, CA, USA) was used to design the multiplexed SNP Mass EXTEND assay. Genotyping was performed using a Sequenom MassARRAY RS1000 (Sequenom, Inc.) according to the manufacturerfacturerufa [30 ]. The SequenomTyper 4.0 Software 4.Sequenom, Inc.) was used to manage and analyze the data [31 (link)]. Based on these results, the following five SNPs were selected: rs6089953, rs6010620, rs6010621, rs4809324, and rs2297441. The SNP data are shown in Table 3.

We reviewed the literatures related to association between TERT and TERC polymorphisms and tumors of urinary system and selected SNPs in TERT and TERC with the minor allele frequencies (MAF) ≥5% in Asian by using HapMap database [22 (link)–24 (link), 38 ]. In addition, the correlation between chosen SNPs and RCC in Chinese Han population has not been reported before. Genomic DNA was extracted from whole blood samples using the Gold Mag-Mini Whole Blood Genomic DNA Purification Kit (version 3.0; TaKaRa, Japan) [39 ]. The DNA concentration was measured by spectrometry (DU530 UV/VIS spectrophotometer, Beckman Instruments, Fullerton, CA, USA). The Sequenom MassARRAY Assay Design 3.0 software (Sequenom, Inc, San Diego, CA, USA) was used to design the multiplexed SNP Mass EXTEND assay. Genotyping was performed using a Sequenom MassARRAY RS1000 (Sequenom, Inc.) in accordance with the manufacturer's protocol. Sequenom Typer 4.0 software was used to perform data management and analyses [40 ]. The primers corresponding to each SNP are listed in Table 7. Based on these results, six SNPs including rs35073794, rs10936599, rs10069690, rs2242652, rs2853677, rs2853676 were selected.

Candidate SNPs were selected from among previously published polymorphisms associated with CC. Validated SNPs were selected with a MAF > 5% in the HapMap Asian population [23 ]. Venous blood samples (5 ml) were collected from each patient during laboratory examination. Genomic DNA was extracted from whole blood samples using a Gold Mag-Mini Whole Blood Genomic DNA Purification Kit (version 3.0; TaKaRa, Japan) [24 (link)] and stored at -80°C after centrifugation. DNA concentrations were evaluated using spectrometry (DU530 UV/VIS spectrophotometer, Beckman Instruments, Fullerton, CA, USA). We used Sequenom MassARRAY Assay Design 3.0 Software to design the Multiplexed SNP MassEXTEND assays [25 ]. SNP genotyping was done with a Sequenom MassARRAY RS1000 using the standard protocol recommended by the manufacturer [25 ]. The primer sequences used for genotyping are listed in Table 4. Data management and analyses were performed using Sequenom Typer 4.0 software as previously described [25 , 26 (link)].