Ma5 15502

Manufactured by Thermo Fisher Scientific

The MA5-15502 is a precision balance that can measure weights up to 15,500 grams with an accuracy of 0.01 grams. It features a large stainless steel weighing platform and a backlit LCD display for easy readability.

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Lab products found in correlation

Ripa lysis buffer, by Cell Signaling Technology (1 mentions) Ab2413, by Abcam (1 mentions) Protein assay dye, by Bio-Rad (1 mentions) Ab76055, by Abcam (1 mentions) Gtx627408, by GeneTex (1 mentions) Ab5694, by Abcam (1 mentions) Ab32536, by Abcam (1 mentions) Alexa fluor 555, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 tyramide superboost kit, by Thermo Fisher Scientific (1 mentions) Axio scan z1, by Zeiss (1 mentions) Vectashield h 1000, by Vector Laboratories (1 mentions) Aho0832, by Thermo Fisher Scientific (1 mentions) S1699, by Agilent Technologies (1 mentions)

2 protocols using ma5 15502

Protein Expression Analysis by Western Blot

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Cellular protein lysates were prepared by homogenization with 1× RIPA lysis buffer (Cell Signaling Technology, Billerica, MA). Protein concentrations were measured using a protein assay dye (Bio-Rad Laboratories, Hercules, CA). SDS-PAGE and immunoblotting analysis were performed as described previously^{3 (link)}. The detecting antibodies were raised against E-cadherin (ab76055, abcam), vimentin (2707-1 epitomics), α-SMA (ab5694, abcam), fibronectin (ab2413, abcam), WNT5A (MA5-15502, Invitrogen), β-catenin (13-8400, Invitrogen), NF-κB (ab32536, abcam), MMP-9 (13667, CST), and GAPDH (GTX627408, GeneTex).

Chung Y.H., Cheng Y.T., Kao Y.H., Tsai W.C., Huang G.K., Chen Y.T., Shen Y.C., Tai M.H, & Chiang P.H. (2022). MiR-26a-5p as a useful therapeutic target for upper tract urothelial carcinoma by regulating WNT5A/β-catenin signaling. Scientific Reports, 12, 6955.

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Immunofluorescence Staining of STAT1, WNT5A, and TP63

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Paraffin-embedded tissue sections were deparaffinized in three changes of xylene and ethanol. Heat-mediated antigen retrieval was performed (Dako S1699, pH 6.0) and tissue slides were permeabilized with 0.5% Triton X-100 solution for 10 minutes at room temperature and then blocked with appropriate serum according to the species of secondary antibody for 1 hour at room temperature. For STAT1 staining (AHO0832, Invitrogen, 1:800), Alexa Fluor 488 Tyramide Super Boost Kit was used (B40941, Invitrogen) and antibody was incubated at 37°C for 30 minutes. For WNT5A (MA5–15502, Invitrogen, 1:800) and TP63 (39692, Cell Signaling Technology, 1:900) staining, samples were incubated at 37°C for 30 minutes. Sample slides were then washed in PBS three times and incubated with DyLight 649 (DI-2649, Vector, 1:100) and Alexa Fluor 555 (A31572, Invitrogen, 1:300) conjugated secondary antibodies for an hour at room temperature. Nuclei were counterstained with DAPI and samples mounted with Vectashield (H1000, Vector Laboratories) and examined using Zeiss Axio Scan.Z1 automated fluorescence slide scanner.

Clarke M., Mackay A., Ismer B., Pickles J.C., Tatevossian R.G., Newman S., Bale T.A., Stoler I., Izquierdo E., Temelso S., Carvalho D.M., Molinari V., Burford A., Howell L., Virasami A., Fairchild A.R., Avery A., Chalker J., Kristiansen M., Haupfear K., Dalton J.D., Orisme W., Wen J., Hubank M., Kurian K.M., Rowe C., Maybury M., Crosier S., Knipstein J., Schüller U., Kordes U., Kram D.E., Snuderl M., Bridges L., Martin A.J., Doey L.J., Al-Sarraj S., Chandler C., Zebian B., Cairns C., Natrajan R., Boult J.K., Robinson S.P., Sill M., Dunkel I.J., Gilheeney S.W., Rosenblum M.K., Hughes D., Proszek P.Z., Macdonald T.J., Preusser M., Haberler C., Slavc I., Packer R., Ng H.K., Caspi S., Popović M., Kotnik B.F., Wood M.D., Baird L., Davare M.A., Solomon D.A., Olsen T.K., Brandal P., Farrell M., Cryan J.B., Capra M., Karremann M., Schittenhelm J., Schuhmann M.U., Ebinger M., Dinjens W.N., Kerl K., Hettmer S., Pietsch T., Andreiuolo F., Driever P.H., Korshunov A., Hiddingh L., Worst B.C., Sturm D., Zuckermann M., Witt O., Bloom T., Mitchell C., Miele E., Colafati G.S., Diomedi-Camassei F., Bailey S., Moore A.S., Hassall T.E., Lowis S.P., Tsoli M., Cowley M.J., Ziegler D.S., Karajannis M.A., Aquilina K., Hargrave D.R., Carceller F., Marshall L.V., von Deimling A., Kramm C.M., Pfister S.M., Sahm F., Baker S.J., Mastronuzzi A., Carai A., Vinci M., Capper D., Popov S., Ellison D.W., Jacques T.S., Jones D.T, & Jones C. (2020). Infant high grade gliomas comprise multiple subgroups characterized by novel targetable gene fusions and favorable outcomes. Cancer discovery, 10(7), 942-963.

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