Lambda ls system

Manufactured by Sutter Instruments

Sourced in United States

The Lambda-LS system is a laboratory equipment product designed to perform spectrophotometric measurements. It is capable of measuring the absorbance or transmittance of samples across a range of wavelengths. The core function of the Lambda-LS system is to provide users with precise and reliable data on the optical properties of various materials and substances.

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Lab products found in correlation

Eclipse te2000 u, by Nikon (5 mentions) Metafluor 6, by Molecular Devices (4 mentions) Super fluor 20 0.75 na, by Teledyne (4 mentions) Lambda 10 2 optical filter change, by Sutter Instruments (4 mentions) Fura 2 am, by Thermo Fisher Scientific (3 mentions) Eclipse ti u, by Nikon (3 mentions) Nis elements software, by Nikon (3 mentions) Lambda 10 to 2 optical filter change, by Sutter Instruments (3 mentions) Eclipse te2000 u inverted microscope, by Nikon (2 mentions) Coolsnap hq, by Teledyne (2 mentions) Coolsnaphq es2, by Teledyne (2 mentions) Cool snap es2, by Teledyne (2 mentions) Cool snaphq photometrics camera, by Teledyne (1 mentions) Metamorph version 6, by Molecular Devices (1 mentions)

Close spelling variants of this product

Lambda LS (21 citations) Lambda LS Xenon Arc Lamp Light Source System (2 citations)

10 protocols using lambda ls system

Measuring Intracellular Calcium Dynamics

Cited 2 times

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Dorsal root ganglion neurons were prepared as previously
described^{27 (link)} and
were loaded for 30 minutes at 37°C with 3 μM Fura-2AM (Cat#
F1221, Thermo Fisher, stock solution prepared at 1mM in DMSO, 0.02% pluronic
acid, Cat#P-3000MP, Life technologies) to follow changes in intracellular
calcium([Ca²⁺]_c) in a standard bath
solution containing 139 mM NaCl, 3 mM KCl, 0.8 mM MgCl₂, 1.8 mM
CaCl₂, 10 mM Na HEPES, pH 7.4, 5 mM glucose exactly as
previously described^{28 (link)}Fluorescence imaging was performed with an inverted microscope,
NikonEclipseTi-U (Nikon Instruments Inc., Melville,
NY), using objective Nikon Fluor 4X and a Photometrics cooled CCD camera
Cool SNAP ES² (Roper
Scientific, Tucson, AZ) controlled by Nis Elements software (version 4.20,
Nikon Instruments). The excitation light was delivered by a Lambda-LS system
(Sutter Instruments, Novato, CA). The excitation filters (340 ± 5 and
380 ± 7) were controlled by a Lambda 10 to 2 optical filter change
(Sutter Instruments). Fluorescence was recorded through a 505-nm dichroic
mirror at 535 ± 25 nm. To minimize photobleaching and phototoxicity,
the images were taken every ~10 seconds during the time-course of the
experiment using the minimal exposure time that provided acceptable image
quality. The changes in [Ca²⁺]_c were monitored by
following a ratio of F₃₄₀/F₃₈₀, calculated after
subtracting the background from both channels.

Musharrafieh R., Zhang J., Tuohy P., Kitamura N., Bellampalli S.S., Hu Y., Khanna R, & Wang J. (2019). Discovery of quinoline analogs as potent antivirals against enterovirus D68 (EV-D68). Journal of medicinal chemistry, 62(8), 4074-4090.

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Mitochondrial Motility in Striatal Neurons

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Mitochondrial motility in striatal cultured neurons was assessed at 37 °C using wide-field fluorescence microscopy. Mitochondrial traffic was recorded with a Nikon Eclipse TE2000-U inverted microscope using a Nikon objective Nikon CFI Plan Apo 100 × 1.4 NA and Photometrics Cool SNAP_HQ camera (Roper Scientific, Tucson, AZ, USA) controlled by MetaMorph software 6.3 (Molecular Devices, Downingtown, PA, USA). The excitation light (480 ± 20 nm) was delivered by a Lambda-LS system (Sutter Instruments, Novato, CA, USA), and fluorescence was measured through a 505 nm dichroic mirror at 535 ± 25 nm. The images were acquired during the time-course of the experiment (5 min) with a frequency of 1 Hz. The motility of neuronal mitochondria was analyzed after constructing kymographs using NIH ImageJ 1.53a software.

Brustovetsky T., Khanna R, & Brustovetsky N. (2021). Involvement of CRMP2 in Regulation of Mitochondrial Morphology and Motility in Huntington’s Disease. Cells, 10(11), 3172.

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Mitochondrial Dynamics in Cortical Neurons

Cited 1 time

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Mitochondrial traffic in cortical neurons in culture was evaluated at 37 °C employing wide-field time-laps fluorescence microscopy as described [17 (link)]. Mitochondrial motility was documented with an inverted microscope Nikon Eclipse TE2000-U utilizing Nikon CFI Plan Apo 100× 1.4 NA objective and Cool SNAP_HQ Photometrics camera (Roper Scientific, Tucson, AZ, USA) managed by MetaMorph software 6.3 (Molecular Devices, Downingtown, PA, USA). The excitation light (480 ± 20 nm) was provided by a Lambda-LS system (Sutter Instruments, Novato, CA, USA), and fluorescence was recorded with a 505 nm dichroic mirror at 535 ± 25 nm. The images were captured at 1 Hz during the whole duration of the experiment (5 min). Mitochondrial traffic was evaluated following construction of kymographs with NIH ImageJ software (version 1.53a).

Brustovetsky T., Khanna R, & Brustovetsky N. (2023). CRMP2 Participates in Regulating Mitochondrial Morphology and Motility in Alzheimer’s Disease. Cells, 12(9), 1287.

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Fura-2 Calcium Imaging of DRG Neurons

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DRG neurons were loaded at 37°C with 3 μM Fura-2AM (K_d=25 μM, λ_ex 340, 380 nm/λ_emi 512 nm) to follow changes in intracellular calcium ([Ca²⁺]_c) in a standard bath solution containing 139 mM NaCl, 3 mM KCl, 0.8 mM MgCl₂, 1.8 mM CaCl₂, 10 mM NaHEPES, pH 7.4, 5 mM glucose exactly as previously described [13 ]. Fluorescence imaging was performed with an inverted microscope, Nikon Eclipse TE2000-U, using objective Nikon Super Fluor 20× 0.75 NA and a Photometrics cooled CCD camera CoolSNAPHQ (Roper Scientific, Tucson, AZ) controlled by MetaFluor 6.3 software (Molecular Devices, Downingtown, PA). The excitation light was delivered by a Lambda-LS system (Sutter Instruments, Novato, CA). The excitation filters (340 ± 5 and 380 ± 7) were controlled by a Lambda 10-2 optical filter change (Sutter Instruments). Fluorescence was recorded through a 505 nm dichroic mirror at 535 ± 25 nm. To minimize photobleaching and phototoxicity, the images were taken every ~5 seconds during the time-course of the experiment using the minimal exposure time that provided acceptable image quality. The changes in [Ca²⁺]_c were monitored by following a ratio of F340/F380, calculated after subtracting the background from both channels.

Xie J.Y., Chew L.A., Yang X., Wang Y., Qu C., Wang Y., Federici L.M., Fitz S.D., Ripsch M.S., Due M.R., Moutal A., Khanna M., White F.A., Vanderah T.W., Johnson P.L., Porreca F, & Khanna R. (2016). Sustained relief of ongoing experimental neuropathic pain by a CRMP2 peptide aptamer with low abuse potential. Pain, 157(9), 2124-2140.

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Fluorescence Imaging of Lipid Order and Peptide Localization

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Fluorescence imaging was performed with an inverted microscope, Nikon Eclipse TE2000-U, using objective Nikon Super Fluor 20× 0.75 NA and a Photometrics cooled CCD camera CoolSNAPHQ-ES2 (Roper Scientific, Tucson, AZ, USA) controlled by MetaFluor 6.3 software (Molecular Devices, Downingtown, PA, USA). The excitation light was delivered by a Lambda-LS system (Sutter Instruments, Novato, CA, USA). The excitation filters were controlled by a Lambda 10-2 optical filter change (Sutter Instruments). Twenty images of each condition were systematically recorded randomly, using a FITC filter (excitation and emission wavelength 488 nm and 500–550 nm, respectively), which accounted for the liquid ordered contribution of di-4-ANEPPDHQ or the localization of FITC-peptides.

Moutal A., François-Moutal L., Brittain J.M., Khanna M, & Khanna R. (2015). Differential neuroprotective potential of CRMP2 peptide aptamers conjugated to cationic, hydrophobic, and amphipathic cell penetrating peptides. Frontiers in Cellular Neuroscience, 8, 471.

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Fura-2 Calcium Imaging in DRG Neurons

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Dorsal root ganglion neurons were loaded for 30 minutes at 37°C with 3 μM Fura-2AM (Cat# F1221, Thermo Fisher, stock solution prepared at 1mM in DMSO, 0.02% pluronic acid, Cat#P-3000MP, Life Technologies) to follow changes in intracellular calcium([Ca²⁺]_c) in a standard bath solution containing 139 mM NaCl, 3 mM KCl, 0.8 mM MgCl₂, 1.8 mM CaCl₂, 10 mM Na HEPES, pH 7.4, 5 mM glucose exactly as previously described [8 ]. Fluorescence imaging was performed with an inverted microscope, NikonEclipseTi-U (Nikon Instruments Inc., Melville, NY), using objective Nikon Fluor 4X and a Photometrics cooled CCD camera Cool SNAP ES² (Roper Scientific, Tucson, AZ) controlled by Nis Elements software (version 4.20, Nikon Instruments). The excitation light was delivered by a Lambda-LS system (Sutter Instruments, Novato, CA). The excitation filters (340 ± 5 and 380 ± 7) were controlled by a Lambda 10 to 2 optical filter change (Sutter Instruments). Fluorescence was recorded through a 505-nm dichroic mirror at 535 ± 25 nm. To minimize photobleaching and phototoxicity, the images were taken every ~10 seconds during the time-course of the experiment using the minimal exposure time that provided acceptable image quality. The changes in [Ca²⁺]_c were monitored by following a ratio of F₃₄₀/F₃₈₀, calculated after subtracting the background from both channels.

Khanna R., Moutal A., White K.A., Chefdeville A., Negrao de Assis P., Cai S., Swier V.J., Bellampalli S.S., Giunta M.D., Darbro B.W., Quelle D.E., Sieren J.C., Wallace M.R., Rogers C.S., Meyerholz D.K, & Weimer J.M. (2019). Assessment of nociception and related quality of life measures in a porcine model of Neurofibromatosis type 1. Pain, 160(11), 2473-2486.

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Measuring Calcium Dynamics in Dorsal Root Ganglion Neurons

Cited 1 time

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Dorsal root ganglion neurons were loaded at 37°C with 3 μM Fura-2AM (K_d = 25 μM, λ_ex 340, 380 nm/λ_emi 512 nm) to follow changes in intracellular calcium ([Ca²⁺]_c) in a standard bath solution containing 139 mM NaCl, 3 mM KCl, 0.8 mM MgCl₂, 1.8 mM CaCl₂, 10 mM NaHEPES, pH 7.4, 5 mM glucose exactly as previously described.^{9 (link),39 ,40 (link)} Fluorescence imaging was performed with an inverted microscope, Nikon Eclipse TE2000-U, using an objective Nikon Super Fluor 20× 0.75 NA and a Photometrics-cooled CCD camera CoolSNAPHQ (Roper Scientific, Tucson, AZ) controlled by MetaFluor 6.3 software (Molecular Devices, Downingtown, PA). The excitation light was delivered by a Lambda-LS system (Sutter Instruments, Novato, CA). The excitation filters (340 ± 5 nm and 380 ± 7 nm) were controlled by a Lambda 10-2 optical filter change (Sutter Instruments). Fluorescence was recorded through a 505-nm dichroic mirror at 535 ± 25 nm. To minimize photobleaching and phototoxicity, images were taken every ~2.4 seconds during the time course of the experiment using the minimal exposure time that provided acceptable image quality. The changes in [Ca²⁺]_c were monitored by following a ratio of F340/F380, calculated after subtracting the background from both channels.

François-Moutal L., Wang Y., Moutal A., Cottier K.E., Melemedjian O.K., Yang X., Wang Y., Ju W., Largent-Milnes T.M., Khanna M., Vanderah T.W, & Khanna R. (2015). A membrane-delimited N-myristoylated CRMP2 peptide aptamer inhibits CaV2.2 trafficking and reverses inflammatory and postoperative pain behaviors. Pain, 156(7), 1247-1264.

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Fluorescence Imaging of Lipid Domains

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Fluorescence imaging was performed with an inverted microscope, Nikon Eclipse TE2000-U, using objective Nikon Super Fluor 20× 0.75 NA and a Photometrics-cooled CCD camera CoolSNAPHQ ES2 (Roper Scientific, Tucson, AZ) controlled by MetaFluor 6.3 software (Molecular Devices, Downingtown, PA). The excitation light was delivered by a Lambda-LS system (Sutter Instruments, Novato, CA). The excitation filters were controlled by a Lambda 10-2 optical filter change (Sutter Instruments). Twenty images of each condition were systematically recorded randomly, using an FITC filter (excitation and emission wavelength 488 nm and 500–550 nm, respectively), which accounted for the liquid-ordered contribution of di-4-ANEPPDHQ or the localization of FITC-peptides. The freeware image analysis program ImageJ (http://rsb.info.nih.gov/ij/) was used for quantifying lipid domains. All images were corrected for background by subtracting the average background fluorescence (areas within the field of view not containing vesicles) from the region of interest.

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Mitochondrial Motility Imaging in Neurons

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Mitochondrial motility in striatal cultured neurons was assessed at 37 °C using wide-field fluorescence microscopy. Mitochondrial traffic was recorded with a Nikon Eclipse TE2000-U inverted microscope using a Nikon objective Nikon CFI Plan Apo 100× 1.4 NA and Photometrics Cool SNAP_HQ camera (Roper Scientific, Tucson, AZ, USA) controlled by MetaMorph, version 6.3 software (Molecular Devices, Downingtown, PA, USA). The excitation light (480 ± 20 nm) was delivered by a Lambda-LS system (Sutter Instruments, Novato, CA, USA) and fluorescence was measured through a 505 nm dichroic mirror at 535 ± 25 nm. The images were acquired during the time-course of the experiment (5 min) with a frequency of 1 Hz. The motility of neuronal mitochondria was analyzed after constructing kymographs using NIH ImageJ, version 1.53a software.

Brustovetsky T., Khanna R, & Brustovetsky N. (2021). CRMP2 Is Involved in Regulation of Mitochondrial Morphology and Motility in Neurons. Cells, 10(10), 2781.

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Measuring Intracellular Calcium in DRG Neurons

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Dorsal root ganglion neurons were loaded for 30 minutes at 37˚C with 3 µM Fura-2AM (Cat# F1221, Thermo Fisher, stock solution prepared at 1mM in DMSO, 0.02% pluronic acid, Cat#P-3000MP, Life Technologies) to follow changes in intracellular calcium([Ca²⁺]_c) in a standard bath solution containing 139 mM NaCl, 3 mM KCl, 0.8 mM MgCl₂, 1.8 mM CaCl₂, 10 mM Na HEPES, pH 7.4, 5 mM glucose, exactly as previously described [9 ]. Fluorescence imaging was performed with an inverted microscope, NikonEclipseTi-U (Nikon Instruments Inc., Melville, NY), using objective Nikon Fluor 4X and a Photometrics cooled CCD camera Cool SNAP ES² (Roper Scientific, Tucson, AZ) controlled by Nis Elements software (version 4.20, Nikon Instruments). The excitation light was delivered by a Lambda-LS system (Sutter Instruments, Novato, CA). The excitation filters (340 ± 5 nm and 380 ± 7 nm) were controlled by a Lambda 10 to 2 optical filter change (Sutter Instruments). Fluorescence was recorded through a 505-nm dichroic mirror at 535 ± 25 nm. To minimize photobleaching and phototoxicity, the images were taken every ~10 seconds during the time-course of the experiment using the minimal exposure time that provided acceptable image quality. The changes in [Ca²⁺]_c were monitored by following a ratio of F₃₄₀/F₃₈₀, calculated after subtracting the background from both channels.

Bellampalli S.S., Ji Y., Moutal A., Cai S., Kithsiri Wijeratne E.M., Gandini M.A., Yu J., Chefdeville A., Dorame A., Chew L.A., Madura C.L., Luo S., Molnar G., Khanna M., Streicher J.M., Zamponi G.W., Leslie Gunatilaka A.A, & Khanna R. (2019). Betulinic acid, derived from the desert lavender Hyptis emoryi, attenuates paclitaxel-, HIV-, and nerve injury-associated peripheral sensory neuropathy via block of N- and T-type calcium channels. Pain, 160(1), 117-135.

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