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Syncropatch 768 pe platform

Manufactured by Nanion Technologies
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Sourced in Germany

The Syncropatch 768 PE platform is a high-throughput automated patch clamp system designed for screening and characterizing ion channel activity. The system is capable of performing parallel electrophysiological recordings from up to 768 cells simultaneously, enabling efficient and reliable data collection.

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Lab products found in correlation

Ezogabine, by Merck Group (1 mentions) Pires2 egfp, by BD (1 mentions) Xe991, by Abcam (1 mentions)

2 protocols using syncropatch 768 pe platform

1

Automated Patch-Clamp Recordings of Cell Lines

Cited 1 time
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Automated
patch-clamp recordings
were performed at room temperature using the Syncropatch 768 PE platform
(Nanion Technologies) as previously described.23 (link) Eight-hole, 384-well recording chips with medium resistance
(2–4 MΩ) were used in this study. The external solution
contained (in mM) 120 NaCl, 20 CsCl, 10 BaCl2, 1 MgCl2, 15 HEPES, and 5 glucose (pH 7.4). The composition of the
internal solution was (in mM) 80 CsF, 50 NMDG, 10 HEPES, 5 BAPTA,
10 phosphocreatine, 2 MgATP, 0.5 Na2GTP, and 0.1 leupeptin,
(pH 7.2–7.3). Whole-cell currents were recorded in a whole-cell
configuration at 0 mV, 250 ms after the start of the voltage pulse
from a holding potential of −60 mV before and after addition
of various concentrations of compounds or vehicle. Whole-cell currents
were not leak subtracted. The contribution of background currents
was determined by recording whole-cell currents at the end of the
experiment after addition of CdCl2 (5 mM). Only CdCl2-sensitive currents were used for analysis.
Yun J., Jeong D., Xie Z., Lee S., Kim J., Surmeier D.J., Silverman R.B, & Kang S. (2022). Palladium-Catalyzed α-Arylation of Cyclic β-Dicarbonyl Compounds for the Synthesis of CaV1.3 Inhibitors. ACS Omega, 7(16), 14252-14263.
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2

Automated Patch Clamp of KCNQ2/3 Channels

Cited 1 time
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Methods for expression and recording in CHO cells were previously described in detail (Vanoye et al., 2022 (link)). Human KCNQ2 cDNA (GenBank accession number NM_172108) in pIRES2-EGFP (BD Biosciences-Clontech, Mountain View, CA, USA) was used as template for in vitro mutagenesis. A stable line expressing human KCNQ3 (GenBank accession number NM_004519) was generated as described and maintained under dual selection with Zeocin (100 μg/ml) and hygromycin B (600 μg/ml). Plasmids with WT KCNQ2 or G256W cDNA were introduced into the KCNQ3-expressing CHO cell stable line by electroporation (Maxcyte STX; MaxCyte Inc., Gaithersburg, MD, USA). Automated patch clamp recording was performed using the Syncropatch 768 PE platform using PatchController384 V.1.3.0 software (Nanion Technologies, Munich, Germany). Pulse protocols were performed before and after addition of ezogabine (10 μM, Sigma-Aldrich) and, subsequently, XE-991 (25 μM, Abcam, Cambridge, MA; or TOCRIS, Minneapolis, MN). Currents reported are XE-991-sensitive currents, calculated by subtraction.
Abreo T.J., Thompson E.C., Madabushi A., Soh H., Varghese N., Vanoye C.G., Springer K., Park K.L., Johnson J., Sims S., Ji Z., Chavez A.G., Jankovic M.J., Habte B., Zuberi A., Lutz C., Wang Z., Krishnan V., Dudler L., Einsele-Scholz S., Noebels J.L., George AL J.r., Maheshwari A., Tzingounis A.V, & Cooper E.C. (2024). Plural molecular and cellular mechanisms of pore domain KCNQ2 encephalopathy. bioRxiv.
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