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Anti sox10 antibody

Manufactured by Santa Cruz Biotechnology
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The Anti-SOX10 antibody is a laboratory tool used to detect the presence of the SOX10 protein in biological samples. SOX10 is a transcription factor that plays a crucial role in the development and function of various cell types, including neural crest cells and Schwann cells. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to identify and analyze the expression of SOX10 in research applications.

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Lab products found in correlation

Anti crtc1, by Cell Signaling Technology (1 mentions) Anti β catenin, by Cell Signaling Technology (1 mentions) Proteinase k, by Merck Group (1 mentions) Sybr green, by Qiagen (1 mentions) Anti creb antibody, by Cell Signaling Technology (1 mentions) Anti histone h3 antibody, by Abcam (1 mentions) Anti irf1 antibody, by Cell Signaling Technology (1 mentions) Anti mitf antibody, by Santa Cruz Biotechnology (1 mentions) Anti irf4 antibody, by Santa Cruz Biotechnology (1 mentions) Anti α tubulin antibody, by Merck Group (1 mentions) Anti neun antibody, by Cell Signaling Technology (1 mentions) Mowiol, by Merck Group (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Cyanine3 (cy3), by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

Mouse anti-SOX10 antibody Sox10 antibody Anti-SOX10 SOX10

6 protocols using anti sox10 antibody

1

ChIP Assay for Transcription Factor Binding Analysis

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ChIP assay was performed according to the protocol supplied with a premix kit (Merck, 17-295). B16-F0 cells were reacted with 1% formaldehyde for 10 min to cross-link between DNA and proteins. Cell extracts were sonicated to yield chromatin fragments with about 200-500 base pairs (bp) in size. Chromatin fragments were incubated with anti-CRTC1 (Cell Signaling, 2587), anti-β-catenin (Cell Signaling, 9562) or anti-SOX10 antibody (Santa Cruz, sc-17342) at 4℃ overnight, and precipitated with protein A-sepharose bead-sheared salmon sperm slurry for 4 h. To reverse the cross-links, ChIP elutes were incubated with 80 mM NaCl for 6 h at 65℃, and supplemented with 40 mM Tris-HCl containing 0.4 mg/ml proteinase K (Sigma-Aldrich, P2308) and 10 mM EDTA in another incubation for 1 h at 45℃. Input and precipitated DNAs were subjected to quantitative PCR encompassing CREB-, LEF1- or SOX10-responsive element at the MITF-M promoter in mice. Nucleotide sequences of PCR primers are described in Table S2. Quantitative PCR with SYBR green (Qiagen 204054) was subjected to 40 cycles in a condition of denaturation for 10 s at 95℃ and combined annealing-DNA extension for 30 s at 60℃.
Yun C.Y., Hong S.D., Lee Y.H., Lee J., Jung D.E., Kim G.H., Kim S.H., Jung J.K., Kim K.H., Lee H., Hong J.T., Han S.B, & Kim Y. (2019). Nuclear Entry of CRTC1 as Druggable Target of Acquired Pigmentary Disorder. Theranostics, 9(3), 646-660.
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2

ChIP Assay in Rat Schwann Cells

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We performed ChIP assays in primary rat Schwann cells with an EZ ChIP kit (Upstate) as described by the manufacturer. Briefly, we obtained DNA, crosslinked to protein with formaldehyde, and sheared the DNA by pulsed ultra-sonication. For immunoprecipitation, we used an anti-SOX10 antibody (Santa Cruz) or a rabbit IgG antibody (negative control). Primer sets, spanning the identified response element, ranged from −421 to −157 bp (site AB), from −421 to −224 bp (site A), from −285 to −46 bp (site B), and from −78 to +169 bp (site D) relative to the transcriptional start site.
Fujiwara S., Hoshikawa S., Ueno T., Hirata M., Saito T., Ikeda T., Kawaguchi H., Nakamura K., Tanaka S, & Ogata T. (2014). SOX10 Transactivates S100B to Suppress Schwann Cell Proliferation and to Promote Myelination. PLoS ONE, 9(12), e115400.
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3

ChIP-PCR of CREB and SOX10

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Cells were incubated with 1% formaldehyde (Sigma-Aldrich, F8775) to cross-link between DNA and proteins, and sonicated to yield chromatin fragments with about 200-500 base pairs. Chromatin fragments were reacted with anti-CREB antibody (Cell Signaling, 9197) or anti-SOX10 antibody (Santa Cruz, sc-17342) at 4℃ overnight, and precipitated with protein A-sepharose bead-sheared salmon sperm slurry (Merck, 17-295) for 4 h. Input and precipitated DNAs were subjected to PCR encompassing CREB- or SOX10-responsive element at the MITF-M promoter. Nucleotide sequences of PCR primers are previously described 35 (link). PCR products were resolved on agarose gels by electrophoresis and stained with EcoDye.
Yun C.Y., Roh E., Kim S.H., Han J., Lee J., Jung D.E., Kim G.H., Jung S.H., Cho W.J., Han S.B, & Kim Y. (2020). Stem Cell Factor-Inducible MITF-M Expression in Therapeutics for Acquired Skin Hyperpigmentation. Theranostics, 10(1), 340-352.
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4

ChIP-qPCR Analysis of PD-L1, IRF1, and MIA

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ChIP assays were performed as described previously (20 (link)). Antibody used was anti-RNA polymerase II serine2 phosphorylation (Abcam, MA, USA) or anti-SOX10 antibody (Santa cruz). Primers used were; 5’- GAG GAA ACT GAG GTC CAA AGA A −3’ (sense) and 5’- GCC CAG ACT TCA GAG CTA ATC −3’ (antisense) for the PD-L1 intron 3 gene region, 5’- GCA AAG GCA TTC CAC TGT TC −3’ (sense) and 5’- GCA TCT TCT ACC TCC ATC CAT AC −3’ (antisense) for the PD-L1 exon 7 gene region, 5’- TGG AGG GAA TCG TGA CCT A −3’ (sense) and 5’- GCT AAG ACC AGG ACG CTA AC −3’ (antisense) for the IRF1 intron 1 gene region, 5’- AGG GCA GCT GAT CTC TTC A −3’ (sense) and 5’- GGC TAA ACC TGG CAC CAA A −3’ (antisense) for the IRF4 intron 4 gene region, and 5’- TGG GCT GTT TCT GGT AAT CA −3’ (sense) and 5’- CAC CTT GGA ATT TCC TGT GC −3’ (antisense) for the MIA gene region.
Yokoyama S., Takahashi A., Kikuchi R., Nishibu S., Lo J., Hejna M., Moon W.M., Kato S., Zhou Y., Hodi F.S., Song J.S., Sakurai H., Fisher D.E, & Hayakawa Y. (2021). SOX10 regulates melanoma immunogenicity through an IRF4-IRF1 axis. Cancer research, 81(24), 6131-6141.
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5

Western Blot Analysis of Transcription Factors

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Whole-cell extracts (10 μg/lane) or nuclear extracts were prepared using the method of Schreiber, E. et al.(19 (link)) and then subjected to Western blotting analysis using anti-SOX10 antibody (Santa Cruz), anti-MITF antibody (C5), anti-IRF4 antibody (Santa Cruz), anti-IRF1 antibody (Cell Signaling Technology, Danvers, MA, USA), anti-α-tubulin antibody (Sigma), or anti-Histone H3 antibody (Abcam). The band intensities were measured by ImageJ and normalized to that of each control lane.
Yokoyama S., Takahashi A., Kikuchi R., Nishibu S., Lo J., Hejna M., Moon W.M., Kato S., Zhou Y., Hodi F.S., Song J.S., Sakurai H., Fisher D.E, & Hayakawa Y. (2021). SOX10 regulates melanoma immunogenicity through an IRF4-IRF1 axis. Cancer research, 81(24), 6131-6141.
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6

Immunohistochemistry Protocol for Cellular Markers

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Immunohistochemistry was performed as described previously slight modifications57 (link),58 (link). Coronal sections were permeabilized with 0.3% Triton X-100/PBS and incubated overnight with primary antibodies in 2% bovine serum albumin (BSA)/PBS, then washed with 0.3% Triton X-100/PBS three times for 5 min each. The sections were incubated with secondary antibodies and Hoechst 33342 in 2% BSA/PBS for 2 h, and then washed with 0.3% Triton X-100/PBS three times for 5 min each. The sections were mounted on slides with Mowiol (Sigma-Aldrich). For immunohistochemistry using anti-Sox9 and anti-Sox10 antibodies, sections were incubated in antigen retrieval solution (10 mM citrate, pH 6.0) for 30 min at 70℃ before the incubation with primary antibodies. Antibodies used here were as follows: anti-NeuN antibody (Cell Signaling Technology), anti-Sox9 antibody (R&D systems), anti-Sox10 antibody (Santa Cruz Biotechnology), anti-Pax6 antibody (Millipore), anti-Tbr2 antibody (R&D systems), secondary antibodies conjugated with Alexa Fluor 647 (Molecular Probe), and secondary antibodies conjugated with Cy3 (Jackson ImmunoResearch).
Hamabe-Horiike T., Kawasaki K., Sakashita M., Ishizu C., Yoshizaki T., Harada S.I., Ogawa-Ochiai K., Shinmyo Y, & Kawasaki H. (2021). Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation. Scientific Reports, 11, 4864.
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