Cd11c cre itgax cre transgenic mice

RIPK1 kinase-dead mutant knock-in mice (Ripk1^kd/kd) in which exon 4 was flanked by loxP sequences were obtained from GlaxoSmithKline^{29 (link)}. Ripk3^ΔR/ΔR mice were generated as reported previously^{28 (link)}. Fadd^−/− mice were a kind gift from J. Zhang (Thomas Jefferson University). Mlkl^−/− mice were a kind gift from J. Murphy (Walter Eliza Hall Institute). Zbp1^−/− mice were obtained from Laboratory of Animal Models for Human Diseases (National Institutes of Biomedical Innovation, Health and Nutrition). CD11c-Cre (Itgax-cre) transgenic mice were obtained from Jackson laboratory. Ripk1^fl/fl mice were a kind gift from M. Pasparakis (University of Cologne). All animals were housed and maintained under specific pathogen-free conditions in the animal facility at Toho University School of Medicine (Ripk1^kd/kd, Ripk3^ΔR/ΔR, Fadd^−/−, and CD11c-Cre mice), Osaka University Graduate School of Medicine (Ripk1^kd/kd, Ripk3^ΔR/ΔR, Fadd^−/−, and CD11c-Cre mice), University of Massachusetts Medical School (Ripk1^kd/kd, Ripk3^ΔR/ΔR, Fadd^−/−, Mlkl^−/−, Zbp1^−/−, Ripk1^fl/fl, and CD11c-Cre mice), and Duke University (Ripk1^kd/kd, Ripk3^ΔR/ΔR, Fadd^−/−, Mlkl^−/−, Zbp1^−/−, Ripk1^fl/fl, and CD11c-Cre mice). All animal experiments were approved by the institutional animal care and use committee.