Anti mouse cd19 1d3

Manufactured by BD

Sourced in United States

The Anti-mouse CD19 (1D3) is a lab equipment product that recognizes the CD19 antigen expressed on mouse B cells. It is designed for use in flow cytometry applications to identify and analyze B cell populations.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

APC rat anti-mouse CD19 clone 1D3 Anti-mouse CD19-APC (clone 1D3, Anti-CD19 (1D3, CD19 (1D3, Anti-CD19 Mouse anti

2 protocols using anti mouse cd19 1d3

Mouse Spleen B Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse spleen B cells overnight cultured in medium or in presence of LPS (100 ng-1 μg/ml, Sigma-Aldrich) were incubated with anti-mouse CD86 (GL1, eBioscience) and anti-mouse CD19 (1D3, BD Bioscience) antibodies and analyzed by FACS canto II (Becton Dickinson, Franklin Lakes, NJ, USA).
IL-1R8 cell surface staining on human cells was performed with biotinylated goat anti-human IL-1R8/SIGIRR (R&D Systems), followed by Alexa-647 conjugated streptavidin (Molecular Probes, Invitrogen), and analyzed with FACS Canto I flow cytometer (BD Bioscience). Diva software (BD Pharmingen) and Flow-jo (Tree Star) were used for data acquisition and analysis, respectively.

Riva F., Ponzoni M., Supino D., Bertilaccio M.T., Polentarutti N., Massara M., Pasqualini F., Carriero R., Innocenzi A., Anselmo A., Veliz-Rodriguez T., Simonetti G., Anders H.J., Caligaris-Cappio F., Mantovani A., Muzio M, & Garlanda C. (2019). IL-1R8 Deficiency Drives Autoimmunity-Associated Lymphoma Development. Cancer immunology research, 7(6), 874-885.

+ Open protocol

+ Expand

Phenotypic Characterization of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The individual performing FACS analysis was not blinded to genotype. Anti-mouse Abs CD4 (GK1.5, BD Pharmingen, Franklin Lakes, NJ, USA) and CD8 (53-6.7, BD Pharmingen) were used for the proliferation assay. Anti-mouse CD19 (1D3, BD Pharmingen), CD1d (1B1, BD Pharmingen), CD5 (53-7.3, BD Pharmingen), CD28 (37.51, BD Pharmingen), CD152 (CTLA-4, UC10-4B9), ICOS (C398-4A, BD Pharmingen), PD-L1 (MIH5, eBioscience), and PD-L2 (TY25, eBioscience) were used for this study. Single-cell suspensions were washed with staining medium (PBS containing 0.1% NaN₃ and 2% FCS). After incubation with mAb and washing with staining buffer, propidium iodide (PI) was added to identify dead cells. FACS data acquisition was performed on a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA) and data were analyzed using isotype control Abs to set quadrants before calculating the percentage of positive cells, using FCS Express (De Novo Software, Los Angeles, CA, USA).

Bodhankar S., Chen Y., Lapato A., Vandenbark A.A., Murphy S.J, & Offner H. (2014). Targeting immune co-stimulatory effects of PD-L1 and PD-L2 might represent an effective therapeutic strategy in stroke. Frontiers in Cellular Neuroscience, 8, 228.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!