All the experimental protocols performed at College of Medicine, Texas A&M University were approved by the Texas A&M University (IACUC: 2022-0083) in accordance with the NIH Guide for the Care and Use and Care of Laboratory Animals. Wild-type C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME). Male and female mice 10–14 weeks of age were made hypertensive by either providing nitro-L-arginine methyl ester hydrochloride (L-NAME) (0.5 mg/ml; Sigma, St. Louis, MO), a nitric oxide synthase inhibitor in the drinking water for 2 weeks, followed by a 2-week washout period and a subsequent 3-week 4% high-salt diet (Teklad Envigo, Huntingdon, U.K.) (SSHTN) or by providing L-NAME in their drinking water for 3 weeks (LHTN) [26 (link),28 (link)]. Control mice received a normal diet and tap water ad libitum. Mice were euthanized after the completion of treatment period by exsanguination under 5% inhalational isoflurane anesthesia with death confirmed by cervical dislocation before tissue collection.
Nitro l arginine methyl ester hydrochloride l name
Manufactured by Merck Group
Sourced in Germany
Nitro-L-arginine methyl ester hydrochloride (L-NAME) is a synthetic compound used as a laboratory reagent. It functions as a nitric oxide synthase inhibitor, a class of molecules that can block the production of nitric oxide in cells.
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Lab products found in correlation
2 protocols using nitro l arginine methyl ester hydrochloride l name
1
Hypertension Induction in C57BL/6J Mice
2
Preparation and Application of Vasoactive Compounds
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A stock solution of 100 mM sodium sulfide (Na2S) (Department of Chemistry and Pharmacy, FAU Erlangen) was diluted to 300 µM with synthetic interstitial fluid (SIF) for topical application or to 900 µM with saline for intravenous infusion. The CGRP receptor antagonist Olcegepant (BIBN4096BS, Boehringer Ingelheim Pharma KG, Germany) was dissolved in acidic pH at 1 mg/ml and then titrated to neutral values. The nitric oxide synthase (NOS) inhibitor nitro-l -arginine methyl ester hydrochloride (l -NAME) (Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany) was dissolved in saline for intravenous infusion at a dose of 10 mg/kg. The NO donor diethylamine-NONOate (DEA-NONOate) (Merck Millipore, Schwalbach, Germany) was dissolved in 10 mM NaOH and diluted in saline immediately before intravenous infusion at a dose of 2.5 µg/kg. An inhibitor of H2S synthesis, oxamic acid (aminooxyacetic acid) (Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany), was dissolved in saline for intravenous infusion (3 mg/kg).
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