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Mda mb 361

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MDA-MB-361 is a human breast carcinoma cell line. It is commonly used in cancer research and drug development studies.

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Lab products found in correlation

Mda mb 468, by Lonza (3 mentions) Bt 549, by Lonza (3 mentions) Hcc1954, by Lonza (3 mentions) Mda mb 231, by Lonza (3 mentions) Mda mb 231, by American Type Culture Collection (3 mentions) Mcf 7, by American Type Culture Collection (3 mentions) Bt549, by American Type Culture Collection (3 mentions) Mda mb 468, by American Type Culture Collection (3 mentions) Dulbecco modified eagle medium (dmem), by Lonza (3 mentions) Mda mb 361, by American Type Culture Collection (3 mentions) Hcc1954, by American Type Culture Collection (3 mentions) Sk br 3, by Lonza (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Lncap, by American Type Culture Collection (2 mentions) Gentamicin, by Lonza (2 mentions) Dulbecco s modified eagle medium dmem, by Lonza (2 mentions) Du145 pc3, by American Type Culture Collection (2 mentions) Skbr3, by American Type Culture Collection (2 mentions) Rpmi 1640 medium, by Lonza (1 mentions) Roswell park memorial institute 1640 medium, by Lonza (1 mentions)

5 protocols using mda mb 361

1

Culturing Human Breast Cancer Cell Lines

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Human breast cell lines MCF‐7, T‐47D, MDA‐MB‐361, MDA‐MB‐231, MDA‐MB‐468, BT‐549, HCC1954, and SK‐BR‐3 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). MCF‐7, MDA‐MB‐361, MDA‐MB‐231, and SK‐BR‐3 cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Lonza, Walkersville, MD, USA). T‐47D, HCC1954, MDA‐MB‐468, and BT‐549 cells were cultured in RPMI 1640 medium (Lonza), supplemented with 10% (v/v) heat‐inactivated fetal bovine serum (SAFC Biosciences, Lenexa, KS, USA), 100 units/mL penicillin, and 100 μg/mL streptomycin. All cells were maintained in a humidified incubator at a constant temperature of 37°C and 5% CO2.
Wang M., Liang L., Lu J., Yu Y., Zhao Y., Shi Z., Li H., Xu X., Yan Y., Niu Y., Liu Z., Shen L, & Zhang H. (2019). Delanzomib, a novel proteasome inhibitor, sensitizes breast cancer cells to doxorubicin‐induced apoptosis. Thoracic Cancer, 10(4), 918-929.
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2

Culturing Breast Cancer Cell Lines

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Human breast cancer cell lines T-47D, MCF7, MDA-MB-361, SK-BR-3, HCC1954, MDA-MB-468, MDA-MB-231, and BT-549 were obtained from the ATCC (Manassas, VA, USA). MCF7, MDA-MB-361, SK-BR-3, and MDA-MB-231 cells were cultured in Dulbecco modified Eagle medium (Lonza, Basel, Switzerland), and T-47D, HCC1954, MDA-MB-468, and BT-549 were maintained in Roswell Park Memorial Institute-1640 medium (Lonza); all media were supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA), penicillin (100 units/mL), and streptomycin (100 mg/mL). All cells were cultured at 37 °C in a humidified atmosphere of 5% carbon dioxide.
Wang H., Yu Y., Jiang Z., Cao W.M., Wang Z., Dou J., Zhao Y., Cui Y, & Zhang H. (2016). Next-generation proteasome inhibitor MLN9708 sensitizes breast cancer cells to doxorubicin-induced apoptosis. Scientific Reports, 6, 26456.
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3

Cell Culture of Prostate and Breast Cancer Lines

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Human prostate cancer (PCa) cell lines LNCaP, DU145 PC3, and 22Rv1 were purchased from the American type culture collection (ATCC; Manassas, VA). Cells were cultured in RPMI-1640 medium (Life Technologies) supplemented with fetal bovine serum (FBS; 10%; Life Technologies). Cell lines were authenticated by genetic biomarkers and mycoplasma tests were performed by PCR amplification (Applied Biological Materials Inc.) and 4′,6-diamidino-2-phenylindole (DAPI) staining. The murine prostate tumor cell line, HMVP2, was derived from the ventral prostate of one year old HiMyc transgenic mice23 (link) and cultured in RPMI-1640 medium containing 10% FBS22 . The human breast cancer cell line MDA-MB-361 (ATCC) was cultured in Dulbecco’s modified Eagle medium (DMEM; Lonza) supplemented with 10% FBS and gentamicin (50 µg/mL, Lonza). All cells were cultured at 37°C in 95% air and 5% CO2.
Cramer S.L., Saha A., Liu J., Tadi S., Tiziani S., Yan W., Triplett K., Lamb C., Alters S.E., Rowlinson S., Zhang Y.J., Keating M.J., Huang P., DiGiovanni J., Georgiou G, & Stone E. (2016). Systemic depletion of serum l-Cyst(e)ine with an engineered human enzyme induces production of reactive oxygen species and suppresses tumor growth in mice. Nature medicine, 23(1), 120-127.
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4

Culturing Breast Cancer Cell Lines

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The human breast cancer cell lines MCF7, T47D, MDA-MB-361, HCC1954, MDA-MB-468, MDA-MB-231, and BT-549 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). MCF7, MDA-MB-361 and MDA-MB-231 cells were routinely cultured in Dulbecco's modified Eagle's medium (DMEM, Lonza, Walkersville, MD, USA), and T-47D, HCC1954, MDA-MB-468 and BT-549 were maintained in RPMI-1640 medium (Lonza), all supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich Co. LLC. St. Louis, MO, USA), 100 units/ml penicillin, and 100 mg/ml streptomycin. All cells were cultured at 37°C in a humidified atmosphere of 5% CO2.
Shi Y., Yu Y., Wang Z., Wang H., Bieerkehazhi S., Zhao Y., Suzuk L, & Zhang H. (2016). Second-generation proteasome inhibitor carfilzomib enhances doxorubicin-induced cytotoxicity and apoptosis in breast cancer cells. Oncotarget, 7(45), 73697-73710.
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5

Cell Culture of Prostate and Breast Cancer Lines

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Human prostate cancer (PCa) cell lines LNCaP, DU145 PC3, and 22Rv1 were purchased from the American type culture collection (ATCC; Manassas, VA). Cells were cultured in RPMI-1640 medium (Life Technologies) supplemented with fetal bovine serum (FBS; 10%; Life Technologies). Cell lines were authenticated by genetic biomarkers and mycoplasma tests were performed by PCR amplification (Applied Biological Materials Inc.) and 4′,6-diamidino-2-phenylindole (DAPI) staining. The murine prostate tumor cell line, HMVP2, was derived from the ventral prostate of one year old HiMyc transgenic mice23 (link) and cultured in RPMI-1640 medium containing 10% FBS22 . The human breast cancer cell line MDA-MB-361 (ATCC) was cultured in Dulbecco’s modified Eagle medium (DMEM; Lonza) supplemented with 10% FBS and gentamicin (50 µg/mL, Lonza). All cells were cultured at 37°C in 95% air and 5% CO2.
Cramer S.L., Saha A., Liu J., Tadi S., Tiziani S., Yan W., Triplett K., Lamb C., Alters S.E., Rowlinson S., Zhang Y.J., Keating M.J., Huang P., DiGiovanni J., Georgiou G, & Stone E. (2016). Systemic depletion of serum l-Cyst(e)ine with an engineered human enzyme induces production of reactive oxygen species and suppresses tumor growth in mice. Nature medicine, 23(1), 120-127.
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