Mouse brains were collected and fixed in 2% paraformaldehyde. After 72 h, the tissues were washed with water and placed in 70% ethanol. Brains were embedded in paraffin and sectioned. Briefly, sections were deparaffinized and rehydrated. Antigen retrieval was performed by boiling for 30 min. Slides were treated with an endogenous peroxidase blocking solution (Vector, USA), followed by another block with 3% bovine serum albumin. Anti-CD68 rabbit monoclonal antibody (Abcam, USA, 1:500) was incubated at 4 C overnight in a humidified chamber. Anti-rabbit IgG secondary antibody was added (Vector), followed by diaminobenzidine substrate for 5 min and counterstained with hematoxylin. All slides were read blinded by a single reader.
Anti rabbit igg secondary antibody
Manufactured by Vector Laboratories
The Anti-rabbit IgG secondary antibody is a laboratory reagent used for the detection and visualization of rabbit primary antibodies in various immunoassay techniques, such as Western blotting, immunohistochemistry, and ELISA. It is a purified, affinity-isolated antibody produced in goat and specifically recognizes the Fc region of rabbit immunoglobulin G (IgG).
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Lab products found in correlation
2 protocols using anti rabbit igg secondary antibody
1
Immunohistochemical Evaluation of CD68 in Mouse Brains
2
Immunohistochemical Evaluation of CD68 in Mouse Brains
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Mouse brains were collected and fixed in 2% paraformaldehyde. After 72 h, the tissues were washed with water and placed in 70% ethanol. Brains were embedded in paraffin and sectioned. Briefly, sections were deparaffinized and rehydrated. Antigen retrieval was performed by boiling for 30 min. Slides were treated with an endogenous peroxidase blocking solution (Vector, USA), followed by another block with 3% bovine serum albumin. Anti-CD68 rabbit monoclonal antibody (Abcam, USA, 1:500) was incubated at 4 C overnight in a humidified chamber. Anti-rabbit IgG secondary antibody was added (Vector), followed by diaminobenzidine substrate for 5 min and counterstained with hematoxylin. All slides were read blinded by a single reader.
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