Fadrozole hydrochloride

3β-HSD enzymatic activities were measured as described^{10 (link)} using ³H-DHEA (PerkinElmer). The tarsal plates were surgically isolated from the upper and lower eyelids. This procedure involved making a small incision near the inner corner of the eyelid, separating skin/subcutaneous tissue from the inner to outer aspect of the lid and then removing skin from the tarsal plates by cutting at the mucocutaneous junction. After these steps, the tarsal plates were cut into slices (0.2 mm thickness) with a tissue chopper (McIlwain laboratory) and transferred into pre-aerated (5% CO₂ and 95% O₂) Hank’s balanced salt solution (HBSS) containing ³H-DHEA (40 nM), dutasteride (10 µM, Cayman), fadrozole hydrochloride (10 µM, Sigma) and 4% propylene glycol. After 30 min at 37 °C, the medium was collected and immediately extracted into ethyl acetate. The extracts were evaporated to dryness under a nitrogen stream at 75 °C. The dry extracts were reconstituted into 40% acetonitrile and subjected to HPLC-connected RI scintillation as described^{10 (link)}.