Atp luciferase based bioluminescence assay kit

Quantification of ATP in cell culture supernatants or aortic tissue was performed by a luciferase-based ATP bioluminescence assay kit, per manufacturer’s instructions (Sigma-Aldrich, St. Louis, MO). Final calculations were evaluated using the standard curve, background subtraction and normalization to controls.

ATP levels were measured in cell culture supernatants, plasma, or aortic tissue using a luciferase-based ATP bioluminescence assay kit, per the manufacturer’s recommendations (Sigma-Aldrich, St. Louis, MO). For extracellular ATP analysis in murine plasma, whole-blood sample of 500 μl was drawn from the infrahepatic inferior vena cava just lateral to the abdominal aorta into a syringe with EDTA. Plasma supernatants were collected after centrifugation for subsequent measurement of ATP content using the bioluminescence assay kit, as previously described^{65 (link)}. Analysis was performed by calculation based on the standard curve, background subtraction, and normalization to controls.

An ATP-luciferase-based bioluminescence assay kit (Sigma, MO, USA) and TD 20/20 luminometer (Turner Designs Sunnyvale, CA, USA) were used to measure ATP levels in the sham and transplant kidney tissue lysates as described earlier [14 (link)].