About 1 g of fresh plant tissue samples (or 0.01–0.05 g freeze-dried samples) were weighed, added 1 ml extract (selected the corresponding extract according to manufacturer's instructions) for ice bath homogenization, then centrifuged 10,000 g at 4°C for 20 min, took the supernatant and placed the supernatant on ice to be tested. And then the determination of antioxidant enzyme activity, MDA, OFR, and H2O2 content were performed with CAT Kit, SOD Kit, POD Kit, MDA Kit, OFR Kit, and H2O2 Kit (Solarbio, Beijing, China) according to the manufacturer's protocol.
Pod kit
Manufactured by Solarbio
Sourced in China
The POD kit is a compact and portable laboratory equipment designed for a variety of applications. It offers a core function of sample preparation and processing in a self-contained unit.
Automatically generated - may contain errors
5 protocols using pod kit
1
Antioxidant Enzyme Activity Measurement
2
Measurement of Peroxidase and Catalase Activities
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3
Determination of Zinc and Antioxidant Enzymes
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The samples were dried in an oven at 80°C to constant weight, then weighed (0.1 g or less) and ground to fine powders. Then the samples were completely digested with 5 mL ternary mixtures of HNO3: H2SO4: HClO4 (10:1:4 (V/V/V)). Appropriate amounts of Zn element standard stock solution were diluted step by step to draw a standard curve. The absorption peaks were determined using the inductively coupled plasma atomic emission spectrometer (ICP, 710-ES, Varian, USA). Finally, the Zn concentration of the samples solution was calculated according to the standard curve, and the Zn mass fraction of the samples was calculated based on the mass.
We determined the content of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), superoxide anion (OFR) and malondialdehyde (MDA) in samples by performing with CAT Kit, POD Kit, SOD Kit, OFR Kit and MDA Kit (Solarbio, Beijing, China) according to the manufacturer's protocol.
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The samples were dried in an oven at 80°C to constant weight, then weighed (0.1 g or less) and ground to fine powders. Then the samples were completely digested with 5 mL ternary mixtures of HNO3: H2SO4: HClO4 (10:1:4 (V/V/V)). Appropriate amounts of Zn element standard stock solution were diluted step by step to draw a standard curve. The absorption peaks were determined using the inductively coupled plasma atomic emission spectrometer (ICP, 710-ES, Varian, USA). Finally, the Zn concentration of the samples solution was calculated according to the standard curve, and the Zn mass fraction of the samples was calculated based on the mass.
We determined the content of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), superoxide anion (OFR) and malondialdehyde (MDA) in samples by performing with CAT Kit, POD Kit, SOD Kit, OFR Kit and MDA Kit (Solarbio, Beijing, China) according to the manufacturer's protocol.
4
Quantifying Antioxidant Enzyme Activities
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SOD and POD activities were determined with a commercially available SOD kit and POD kit (Solarbio Science, Beijing, China), according to the instructions. For SOD assay, absorbance at 560 nm was detected and used for calculating inhibition rate; when the inhibition rate of xanthine oxidase coupling reaction reached 50%, it was defined as one SOD enzyme activity unit. For POD assay, absorbances at 470 nm were detected either at 30 s or at 2 min 30 s after the reaction started; when the variable of absorbance at 470 nm per minute per gram of sample reached 0.01, it was defined as one enzyme activity unit. Both SOD and POD enzyme activities were displayed as U/g fresh weight plant samples.
5
Chlorophyll and Enzyme Dynamics in Wheat Leaves
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Whole flag leaves were collected every 7 d from heading to 28 d to measure the chlorophyll, NR, POD, MDA, and TK contents.
NR: the unit was U·g−1, which was determined by the NR kit of Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China); TK:TK was determined by Shanghai Ruifan Biological Co., Ltd. (Shanghai, China) Plant TK kit, the unit was U·mL−1; malondialdehyde (MDA): The MDA kit was determined by Solarbio’s MDA kit, and the unit was nmol·g−1; POD:POD was determined by Solarbio’s POD kit, and the unit was U·g−1.
NR: the unit was U·g−1, which was determined by the NR kit of Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China); TK:TK was determined by Shanghai Ruifan Biological Co., Ltd. (Shanghai, China) Plant TK kit, the unit was U·mL−1; malondialdehyde (MDA): The MDA kit was determined by Solarbio’s MDA kit, and the unit was nmol·g−1; POD:POD was determined by Solarbio’s POD kit, and the unit was U·g−1.
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