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Mouse il 6 elisa maxtm standard

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The Mouse IL-6 ELISA MAXTM Standard is a laboratory equipment product used for the quantitative measurement of mouse interleukin-6 (IL-6) levels in biological samples. It is a sandwich enzyme-linked immunosorbent assay (ELISA) kit that provides a standardized method for detecting and quantifying this cytokine.

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Mouse IL-6 (28 citations) ELISAs (15 citations) ELISA MAXTM Standard (3 citations)

3 protocols using mouse il 6 elisa maxtm standard

1

Measuring Cytokine Levels in Immunized Mice

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The levels of IL-6 and tumor necrosis factor-alpha (TNF-α) in the serum at day 42 after the first immunization were measured with the Mouse IL-6 ELISA MAXTM Standard and Mouse TNF-α ELISA MAXTM Standard kits (BioLegend) according to the manufacturer’s instructions (Patole et al., 2005 (link); Stolk et al., 2006 (link)).
Yamashita M., Matsumoto K., Endo T., Ukibe K., Hosoya T., Matsubara Y., Nakagawa H., Sakai F, & Miyazaki T. (2017). Preventive Effect of Lactobacillus helveticus SBT2171 on Collagen-Induced Arthritis in Mice. Frontiers in Microbiology, 8, 1159.
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2

Quantifying IL-6 Levels by ELISA

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IL-6 levels in the culture supernatants were determined by Human IL-6 ELISA MAXTM Standard or mouse IL-6 ELISA MAXTM Standard (both from Biolegend) following the manufacturer’s instructions.
Jung B.G., Wang X., Yi N., Ma J., Turner J, & Samten B. (2017). Early Secreted Antigenic Target of 6-kDa of Mycobacterium tuberculosis Stimulates IL-6 Production by Macrophages through Activation of STAT3. Scientific Reports, 7, 40984.
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3

IL-6 Quantification in DC2.4 and RAW 264.7 Cells

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The media of cultured DC2.4 cells and RAW 264.7 cells were harvested and stored at -80°C. The secretion of IL-6 in the culture cell media was measured with enzyme-linked immunosorbent assay kits (Mouse IL-6 ELISA MAXTM Standard, BioLegend). The cytokine concentration was evaluated according to the manufacturer’s protocol.
Yamashita M., Ukibe K., Matsubara Y., Hosoya T., Sakai F., Kon S., Arima Y., Murakami M., Nakagawa H, & Miyazaki T. (2018). Lactobacillus helveticus SBT2171 Attenuates Experimental Autoimmune Encephalomyelitis in Mice. Frontiers in Microbiology, 8, 2596.
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