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Apc cd3 antibody

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The APC-CD3 antibody is a fluorescently labeled monoclonal antibody that binds to the CD3 complex on the surface of T cells. The CD3 complex is essential for T cell activation and signaling. The APC (Allophycocyanin) fluorescent label allows for the identification and analysis of T cells in flow cytometry applications.

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Lab products found in correlation

Annexin 5 fitc, by BioLegend (2 mentions) 7 aad, by BioLegend (2 mentions) Dioc6, by Thermo Fisher Scientific (2 mentions) Model elwd 0, by Nikon (2 mentions) Facscalibur, by BD (2 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Beyotime (1 mentions) Interleukin 2 (il 2), by Beyotime (1 mentions) Porcine peripheral blood lymphocyte separation kit, by Solarbio (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD3-APC (117 citations) APC-conjugated anti-human CD3 antibody (3 citations) APC‐H7‐conjugated anti‐human CD3 antibody (clone SK7; (2 citations) APC anti-human CD3+ monoclonal antibody (2 citations) Purified mouse APC-conjugated anti-human CD3 antibody (2 citations)

4 protocols using apc cd3 antibody

1

Inhibition of T-cell Proliferation by hUC-MSCs

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The hUC-MSCs were seeded in 12-well plates at a density of 5 × 104 cells/well and pretreated with IFN-γ (40 ng/ml) and ATP (2 mM), as described above. hPBMCs (1 × 106 cells/ml) were activated using anti-CD3/CD28 antibodies for 48 h and then cultured with IL-2 (200 U/ml) alone for 48 h. The hPBMCs were incubated with 5 μM CFSE (Beyotime) for 20 min at a concentration of 106 cells/ml. They were protected from light during the entire procedure. After labeling, hPBMCs were stimulated with 200 IU/ml IL-2 (Acrobiosystems) in T-cell culture medium (RPMI 1640 medium supplemented with 10% FBS, 1% penicillin, and 1% glutamine) and co-cultured with or without pretreated hUC-MSCs (hUC-MSC: hPBMC ratio = 1:10) at 37°C and 5% CO2. After 3 d, hPBMCs were collected, and CD3+ T-cell proliferation was measured by flow cytometry as a decrease in CFSE intensity because of cell division from the original population. APC-CD3 antibody was purchased from BD Biosciences.
Yao M., Chen Z., He X., Long J., Xia X., Li Z., Yang Y., Ao L., Xing W., Lian Q., Liang H, & Xu X. (2022). Cross talk between glucose metabolism and immunosuppression in IFN-γ–primed mesenchymal stem cells. Life Science Alliance, 5(12), e202201493.
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2

Porcine PBMC Isolation and T Cell Purification

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Whole blood was collected from newborn piglets, 40-day-old pigs or 120-day-old pigs. Peripheral blood mononuclear cells (PBMCs) were isolated by a density centrifugation using a porcine peripheral blood lymphocyte separation kit (Solarbio) and cultured in RPMI 1640 medium with 10 % FBS (Gibco) and 1% penicillin/streptomycin (20 ng/mL) at 37°C with 5 % CO 2 . Red blood cells (RBCs) were separated as a centrifuged pellet a er purification of PBMCs were washed three times in Dulbeccoʼs phosphate saline (lacking Ca2 + and Mg2 + , DPBS) and stored in Red Cell Storaging Solution (Solarbio) at 4 °C. To isolate T cells, PBMCs were labeled with APC-CD3 antibody (BD Biosciences), incubated with anti-APC microbeads and sorted using MiniMACS Starting kits. Vero E6 cells were kindly provided by the Veterinary Medicine Research Center of the Da Bei Nong Group.
Li J., Chen Y., Liu P., Li Y., Zhang E., Zhang P., & Yang Q. (2020). Red blood cells serve as a vehicle for Coronavirus PEDV transmission.
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3

Evaluating Cell Viability and Niche Cells in CLL

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The percentage of viable cells was determined by staining with 3,3 dihexyloxocarbocyanine iodide (DiOC6; Molecular Probes) and propidium iodide (PI; Molecular Probes), as previously described [38 (link)]. Relative changes in cell numbers were measured by counting cells at high sample flow for 20 seconds on a BD FACSCalibur (BD Biosciences, Franklin Lakes, NJ, USA). The viability of CD3+ T cells was measured in CLL PBMC co-cultures with NLC by flow cytometry after staining with CD3-APC antibody (BD Pharmingen), Annexin-V-FITC, and 7-AAD (Biolegend). To assess the NLC numbers, nonadherent cells were removed by pipetting, the adherent NLC were fixed with absolute methanol for 5 minutes and then stained with modified Giemsa stain (diluted 1:20 in PBS) for 1 hour. NLC were visualized using a phase-contrast microscope (Model ELWD 0.3; Nikon, Melville, NY, USA) with a Ph1 Plan 10 DL/0.25 160/- objective lens. Images were captured with a Nikon D40 digital camera (Nikon Corp) with the help of digiCamControl ver. 1.2.0.0 (http://digicamcontrol.com/). Average numbers of NLC counted in 8 different visual fields are reported.
Kim E., ten Hacken E., Sivina M., Clarke A., Thompson P.A., Jain N., Ferrajoli A., Estrov Z., Keating M.J., Wierda W.G., Bhalla K.N, & Burger J.A. (2019). The BET Inhibitor GS-5829 Targets Chronic Lymphocytic Leukemia Cells and Their Supportive Microenvironment. Leukemia, 34(6), 1588-1598.
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4

Evaluating Cell Viability and Niche Cells in CLL

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The percentage of viable cells was determined by staining with 3,3 dihexyloxocarbocyanine iodide (DiOC6; Molecular Probes) and propidium iodide (PI; Molecular Probes), as previously described [38 (link)]. Relative changes in cell numbers were measured by counting cells at high sample flow for 20 seconds on a BD FACSCalibur (BD Biosciences, Franklin Lakes, NJ, USA). The viability of CD3+ T cells was measured in CLL PBMC co-cultures with NLC by flow cytometry after staining with CD3-APC antibody (BD Pharmingen), Annexin-V-FITC, and 7-AAD (Biolegend). To assess the NLC numbers, nonadherent cells were removed by pipetting, the adherent NLC were fixed with absolute methanol for 5 minutes and then stained with modified Giemsa stain (diluted 1:20 in PBS) for 1 hour. NLC were visualized using a phase-contrast microscope (Model ELWD 0.3; Nikon, Melville, NY, USA) with a Ph1 Plan 10 DL/0.25 160/- objective lens. Images were captured with a Nikon D40 digital camera (Nikon Corp) with the help of digiCamControl ver. 1.2.0.0 (http://digicamcontrol.com/). Average numbers of NLC counted in 8 different visual fields are reported.
Kim E., ten Hacken E., Sivina M., Clarke A., Thompson P.A., Jain N., Ferrajoli A., Estrov Z., Keating M.J., Wierda W.G., Bhalla K.N, & Burger J.A. (2019). The BET Inhibitor GS-5829 Targets Chronic Lymphocytic Leukemia Cells and Their Supportive Microenvironment. Leukemia, 34(6), 1588-1598.
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