Fetal heart tissues were fixed in 4% formalin in PBS and subsequently treated for the histological study by dehydration (increasing alcohol concentrations, from 60% to absolute alcohol), mounting in xylene and immersion in paraffin. Then, the paraffin blocks were cut into 4 mm sections for hematoxylin and eosin staining. The slides were viewed under an Olympus SZX12 inverted microscope and the images were captured under the Olympus U‐CMAD3 camera.
Szx12 inverted microscope
Manufactured by Olympus
The SZX12 inverted microscope is a versatile optical instrument designed for a range of laboratory applications. It features a magnification range of 6.3x to 80x, enabling detailed observation of specimens. The microscope is equipped with an inclined binocular observation tube, providing comfortable viewing for the user. The SZX12 utilizes a high-performance optical system to deliver clear, high-quality images. This product is suitable for various laboratory tasks that require precise magnification and observation of samples.
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Lab products found in correlation
U cmad3 camera, by Olympus (1 mentions)
Alizarin red s solution, by Cyagen (1 mentions)
5 bromo 4 chloro 3 indolyl phosphate nitroblue tetrazolium solution, by Beyotime (1 mentions)
Dp72 digital camera, by Olympus (1 mentions)
Ix71 inverted microscope, by Olympus (1 mentions)
Bx60 stereomicroscope, by Olympus (1 mentions)
3 protocols using szx12 inverted microscope
1
Histological Analysis of Fetal Heart
2
Osteogenic Differentiation Assays
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ALP staining was performed following 7 days osteogenic stimulation. Briefly, cells were rinsed twice with PBS and fixed with 4% formaldehyde for 30 min at room temperature. The fixed cells were stained with 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium solution (Beyotime Institute of Biotechnology, Shanghai, China) for 30 min at room temperature and washed 3 times with PBS. ARS staining was used to assess mineral deposition produced at late-stage bone formation. Briefly, following 14 days osteogenic stimulation, cells were washed twice with PBS, fixed with 4% formaldehyde for 30 min at room temperature, washed twice with PBS and then stained with the Alizarin Red S Solution (Cyagen Biosciences, Inc., Chicago, USA) for 30 min at room temperature. Following ARS staining, the cells were washed 3 times with PBS. Images were captured using an Olympus SZX12 inverted microscope equipped with a digital camera and connected to a PC using MagnaFire 2.0 camera software.
3
Mammary Gland Whole-Mounts and Organoid Imaging
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Mammary gland whole-mounts and transwell filters were imaged with Olympus SZX12 Inverted Microscope. Slides were imaged with Olympus BX60 Stereo Microscope. Organoids, annexin V-FITC-staining, and wound closure assays were imaged with Olympus IX71 Inverted Microscope. All images were acquired by Olympus DP 72 Digital Camera and CellSens software at ambient temperature.
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