Anti crkii

Preparation of cell lysates and immunoblot analysis was performed via established protocols [82 (link)]. Anti-human primary antibodies were implemented at a 1:1, 000–2, 000 dilution and include: anti-NDRG1 (Cat#:ab37897) from Abcam (Cambridge); anti-Src (Cat#:2123), anti-phospho-Src Family (Tyr416; Cat.#:6943), anti-phospho-Src(Tyr527; Cat.#:2105), anti-p130Cas (Cat.#:13846), anti-phospho-p130Cas (Tyr249; Cat.#:4014), anti-phospho-p130Cas (Tyr410; Cat.#:4011), anti-EGF Receptor (Cat.#:2926), anti-phospho-EGF Receptor (Tyr1148; Cat.#:4404), anti-c-Abl (Cat.#:2862), anti-phospho-c-Abl (Tyr245; Cat.#:2861), anti-PAK1 (Cat.#:2602), anti-phospho-PAK1 (Thr423; Cat.#:2606), anti-PTP-PEST (Cat.#:4864), anti-PTP1B (Cat.#:5311), anti-CrkII (Cat.#:3492) and anti-phospho-CrkII (Tyr221; Cat.#:3491) were from Cell Signaling Technology (Boston, MA); and anti-Rac1 (Cat.#:05–389) was from Millipore (Darmstadt). The secondary antibodies (1:10, 000 dilution) included: anti-goat (Cat.#:A5420), anti-rabbit (Cat.#:A6154) and anti-mouse (Cat.#:A4416) antibodies from Sigma-Aldrich. β-actin (1:10, 000; Cat.#:A1978, Sigma-Aldrich) was used as a loading control.

Commercial antibodies included: anti-TrkB (BD Biosciences, New Jersey, NJ, USA; #cat 610102), anti-TrkB (Millipore, Burlington, MA, USA; #cat 07-225), anti-phospho-TrkB (Tyr 515) (Sigma, St Louis, MO, USA; #cat SAB4503785), c-Abl (Sigma #cat A5844), anti-c-Abl (Santa Cruz Biotechnology, Dallas, TX, USA; #cat K-12), anti-c-Abl (Santa Cruz Biotechnology, #cat 24-11), anti-phospho-c-Abl (Tyr 412) (Sigma, #cat C5240), anti-PLC-γ (Cell Signaling, Danvers, MA, USA; #cat 2822), anti-phospho-PLC-γ (Tyr 783) (Cell Signaling, #cat 2821), anti-AKT (Cell Signaling, #cat 9272), anti-phospho-AKT (Ser 473) (Cell Signaling, #cat 9291), anti-ERK (Cell Signaling, #cat 9102), anti-phospho-MAPK (Erk1/2; p42/44; Thr202/Tyr204) (Cell Signaling, #cat 9101), anti-Flag (Sigma, #cat F3040), anti-GAPDH (Santa Cruz, #cat 6C5), β-III Tubulin (Sigma, #cat T8578), anti-MAP2 (Sigma, #cat M3696), anti-phospho-CrkII (Tyr221) (Cell Signaling, #cat 3491), anti-CrkII (Cell Signaling, #cat 3492), Secondary Antibody: Goat anti-Mouse IgG (H + L) and Goat anti-Rabbit IgG (H + L), HRP (Invitrogen, Carlsbad, CA, USA, #cat 31430 and #cat 31460) and secondary antibody Alexa-555, Alexa-488, Alexa-633 and Alexa 547 (1:1000) (Invitrogen, Carlsbad, CA, USA).