Cisplatin solution

Harvested adult worms were washed and incubated with 5 mM H₂O₂ for 30 min, followed by lysis and western blot analysis. For recovery from H₂O₂ exposure, washed worms were put onto seeded NGM plates for the indicated times. Epigallocatechin gallate (EGCG): EGCG (Sigma) containing plates were prepared by spreading 200 µL of 400 µM EGCG dissolved in water on unseeded NGM plates to obtain a final concentration of 5.7 µM. Spots of concentrated OP50 were applied for food after the plates had dried. L3 larvae were transferred onto NGM + EGCG plates for 24 h and transferred onto fresh EGCG plates for another 24 h. Worms were washed 3 times with M9 followed by lysate preparation or exposed to cisplatin and tested for survival. Cisplatin treatment: Cisplatin plates were prepared using MYOB media with 2% agar in which the drug was added at a final concentration of 300 μg/mL. Cisplatin solution (1 mg/mL, Accord Healthcare AB) was added to autoclaved medium after cooling to 52 °C. Young adults (24 h post L4 stage) were collected, washed and incubated on cisplatin plates for 3 or 6 h. Worms were harvested and processed for lysate preparation.

Gemcitabine, AZD7762, Gö6976, romidepsin and givinostat were purchased from Selleck Chemicals. Gemcitabine was dissolved in water, all other compounds were dissolved in dimethyl sulfoxide (DMSO). Cisplatin solution was purchased from Accord Healthcare (London, UK). The reagents were stored at −70 °C until use.