This analysis was performed on a Microflex TOF mass spectrometer (Bruker Daltonics, Bremen, Germany) in linear mode. 2 μL of peptide solution (1 mg mL−1) was mixed with 18 μL of matrix solution (10 mg mL−1), and then the mixture (1 μL) was seeded on a polished steel target (Bruker Daltonics) and dried. α-Cyano-4-hydroxycinnamic acid was used as a matrix, with a laser of 500 shots and 25–30% power.
Microflex tof mass spectrometer
Manufactured by Bruker
Sourced in Germany
The Microflex TOF mass spectrometer is a time-of-flight mass spectrometer designed for high-throughput analysis. It features a compact design and is capable of providing accurate mass measurements of a wide range of analytes.
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Lab products found in correlation
3 protocols using microflex tof mass spectrometer
1
MALDI-TOF Mass Spectrometry Protocol
2
MALDI-TOF Cheese Spectra Acquisition
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The acquisition of the MALDI-TOF spectra from the cheeses was made using a Microflex TOF mass spectrometer (Bruker Daltonics, Germany) equipped with a pulsed N2 laser (337 nm, 3-ns pulse duration). The spectrometer was run with the default parameter settings: positive linear mode, 60 Hz laser frequency, ion source 1 voltage: 20 kV, ion source 2 voltage: 18 kV, and mass range of m/z 1000 to 20,000 using the FlexControl 3.0 software (Bruker Daltonics, Germany). Three independent spectra were collected manually for each cheese sample to verify the mass accuracy and reproducibility, which was always within the range of 0.5 and 1%. The external mass calibration was measured daily using the Protein Calibration Standard I kit (Bruker Daltonics, Bremen, Germany).
3
MALDI-TOF Analysis of Peptides
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The peptide (1 mg mL−1) was mixed with 2,5-dihydroxybenzoic acid or sinapinic acid (1 mg mL−1) at 2 : 18 (v/v), and 1 μL was seeded on the steel target. The experiment was performed on an Microflex TOF mass spectrometer (Bruker Daltonics, Bremen, Germany) in reflectron mode, using an polished steel target (Bruker Daltonics, Bremen, Germany) laser: 500 shots and 25–30% power.
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