Agilent 1260 series liquid chromatography system

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 1260 series liquid chromatography system is a versatile analytical instrument used for the separation, identification, and quantification of chemical compounds in complex mixtures. It is designed to provide reliable and reproducible results through its precision engineering and advanced technology.

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4 protocols using agilent 1260 series liquid chromatography system

HPLC Analysis of Sweetener Compound in Carbonated Beverages

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The HPLC analysis was performed on an Agilent 1260 series liquid chromatography system (Agilent Technologies, Palo Alto, CA, USA) and controlled by the Agilent ChemStation software. All separations were carried out on an Agilent ZORBAX SB-C18 column (250 × 4.6 mm i.d., 5 µm) and a guard column (ZORBAX SB-C18, 12.5 × 4.6 mm i.d., 5 µm) at a column temperature of 35 °C. The detection wavelength was set at 280 nm and the injection volume of all samples was 5.0 µL. The mobile phase consisting of solvent A (methanol) and solvent B (0.1% formic acid solution) was eluted with the following gradient elution program: 0–3 min, 35% A; 3–9 min, 35%–100% A; 9–12 min, 100%–50% A. The flow rate was at 1.0 mL/min. The samples were filtered through a 0.22 µm membrane filter (Navigator Lab Instrument, Tianjin, China) before being injected into HPLC for analysis.
The SBA was prepared with different concentrations of 6.25–1000 ppm in 10.0 mM PBS (pH = 6.0), and a calibration curve for the determination of SBA by HPLC analysis was established. Then, the spiked recovery tests of the carbonated beverage samples (Cola, Sprite, and Fanta) were performed by the HPLC method after spiking with three different concentrations of SBA (final concentrations of 50, 100, and 200 ppm).

Tian T., Zhang W.Y., Zhou H.Y., Peng L.J., Zhou X., Zhang H, & Yang F.Q. (2022). A Catechol-Meter Based on Conventional Personal Glucose Meter for Portable Detection of Tyrosinase and Sodium Benzoate. Biosensors, 12(12), 1084.

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Spectroscopic Analysis of Organic Compounds

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1D and 2D NMR spectra
were acquired in CDCl₃ with a Bruker AVANCE III 400, Bruker
AVANCE III 500, or Bruker AVANCE III 600 spectrometer (Bruker Biospin
AG, Fällanden, Germany) with residual CDCl₃ (δ_H 7.26 ppm, δ_C 77.16 ppm) as the internal
standard. HRESIMS spectra were measured using an Agilent G6520 Q-TOF
mass spectrometer. A Nicolet 6700 spectrometer (Thermo Scientific,
Waltham, MA, USA) was used to obtain the IR spectra. Circular dichroism
spectra
were measured using a JASCO 810 spectrometer. UV spectra were acquired
using a Varian Cary 50 Bio spectrophotometer. Optical rotations were
obtained on a PerkinElmer 241MC polarimeter. Column chromatography
(CC) was performed using commercial silica gel (Sinopharm Chemical
Reagent Co., Ltd., 200–300 and 300–400 mesh) and Sephadex
LH-20 gel (GE Healthcare). Thin-layer chromatography was performed
on precoated silica gel plates [Merck Chemicals (Shanghai) Co., Ltd.,
G60 F-254]. RP-HPLC was performed on an Agilent 1260 series liquid
chromatography system (Agilent, Santa Clara, CA, USA) equipped with
a DAD G1315D detector at 210 nm and an Agilent semi-preparative XDB-C18
column (5 μm, 250 × 9.4 mm). All solvents used for CC were
of analytical grade (Shanghai Chemical Reagent Co., Ltd.) and for
HPLC were of chromatographic grade (Dikma Technologies Inc).

Zeng Z.R., Chen J., Wang H., Zhang H.Y., Li J., Xu B, & Guo Y.W. (2022). Bioactive Cembranoids from the Coral Sarcophyton trocheliophorum of Ximao Island. ACS Omega, 7(45), 41678-41686.

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Quantification of Iohexol and Creatinine

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An Agilent 1260 series liquid chromatography system paired with 6420 triple quadrupole mass spectrometer (Agilent Technologies, Santa Clara, CA) was used to analyze plasma samples. Column temperature was maintained at 20°C. Mobile phase consisted of 0.1% formic acid in water (mobile phase A) and acetonitrile (mobile phase B) for both methods. For analysis of iohexol, a Poroshell 120 analytical column was used (2.7 µm, 100 × 3.0 mm, Part # 695975–302, Agilent Technologies). A gradient method was used to separate the analytes with the following solvent compositions: 5%[A]/95%[B] (0–4 mins), 97%[A]/3%[B] (4.1–7 mins) at a mobile phase flow rate of 0.6 mL/min. Multiple-reaction monitoring mode was used to analyte detection, and the transitions monitored were m/z 821.8 to 803.8 and m/z 826.8 to 808.8 for iohexol and iohexol-d5, respectively.
Creatinine was analyzed as previously reported ^{15 (link)}. In brief, a Poroshell 120 analytical column was used (2.7 µm, 50 × 3.0 mm, Part # 699975–302, Agilent Technologies). A gradient method was used to separate the analytes and mobile phase flow rate was 0.6 mL/min. Multiple-reaction monitoring mode was used to analyte detection, and the transitions monitored were m/z 114.1 to 44.3 and m/z 117.1 to 89.2 for creatinine and creatinine-d3, respectively.

CHANG J., PAIS G.M., MARIANSKI S., VALDEZ K., LESNICKI E., BARRETO E.F., RHODES N.J., YARNOLD P.R, & SCHEETZ M.H. (2023). Iohexol-measured glomerular filtration rate and urinary biomarker changes between vancomycin and vancomycin plus piperacillin-tazobactam in a translational rat model. bioRxiv.

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Characterization of Magnetic Nanocomposites

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The morphologies of magnetic nanocomposites were observed using a SU8010 scanning electron microscope (SEM, Hitachi, Japan) and JEM 2100F transmission electron microscope (TEM, JEOL, Japan). Infrared spectra were recorded with a Nicolet 6700 FT-IR spectrophotometer (Thermo Scientific, USA). Magnetic properties were measured by a LakeShore 7404 vibrating sample magnetometry (VSM, LakeShore, USA).
Quantitative analysis was carried out with an Agilent 1260 series liquid chromatography system (Agilent Technologies, USA). A ChromCore C18 column (4.6 × 250 mm, 5 μm) was chosen for chromatographic separation. 20 μL of samples were injected into the system and eluted with mobile phase of acetonitrile (containing 0.1% formic acid) and water (containing 0.1% formic acid). The gradient program was shown in Table S1, and the detection wavelength was set at 390 nm.

Xie Q.Y., Chen Y., Li C.J., Zhang J.B., Cao X.J, & Lu J. (2024). Ionizable copolymer functionalized magnetic nanocomposite as an adsorbent for boosting the extraction selectivity of aristolochic acids. Journal of Food and Drug Analysis, 32(1), 65-78.

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