Biodoc it

Manufactured by Analytik Jena

Sourced in United States

The BioDoc-It is a compact and versatile imaging system designed for gel documentation and analysis. It features a high-resolution CCD camera, UV and white light illumination, and intuitive software for capturing, processing, and analyzing images of DNA, RNA, and protein gels.

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Lab products found in correlation

Safe imager 2, by Thermo Fisher Scientific (1 mentions) Clariostar plus microplate reader, by BMG Labtech (1 mentions) Agarose gel, by Sinaclon (1 mentions) Ethidium bromide, by Sinaclon (1 mentions) 100 bp dna ladder, by Solis BioDyne (1 mentions)

Spelling variants of this product

BioDoc-It Imaging System (46 citations) BioDoc-It System (7 citations) UVP BioDoc-It Imaging System (4 citations) BioDoc-ItTM System (3 citations) UVP BioDoc-It system (3 citations) UVP Biodoc-It 2 imaging system (2 citations)

4 protocols using biodoc it

Fluorescence-Based DNA Reporter Assay

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The fluorescence generated by
the DNA reporter was measured using a CLARIOstar Plus microplate reader
(BMG LABTECH). At the end of the definitive screening design runs,
20 μL reaction volume was mixed with 80 μL water in each
well of a black and clear flat-bottom 96-well microplate (Greiner).
For sensitivity tests, 1 μL reaction samples were taken and
measured every 10 min. To measure the fluorescent intensity of 6-carboxyfluorescein
(6-FAM), the excitation wavelength was set to 495 nm and the emission
wavelength was set to 520 nm with an 8 nm bandwidth for both. An enhanced
dynamic range (EDR) was used for fluorescence gain. To visually observe
the test tubes, UV light (BioDoc-It, UVP) and a blue-LED transilluminator
(Safe Imager 2.0, Thermo Fisher Scientific) were used.

Malcı K., Walls L.E, & Rios-Solis L. (2022). Rational Design of CRISPR/Cas12a-RPA Based One-Pot COVID-19 Detection with Design of Experiments. ACS Synthetic Biology, 11(4), 1555-1567.

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Plasmid DNA Extraction and Visualization

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EAEC strains grown overnight in Luria broth were used for plasmid DNA extraction by using alkaline lysis method (15 ). Purified plasmid DNA (3 µL) was analyzed by performing gel electrophoresis using 1% agarose gel (SinaClon Co., Iran) stained with ethidium bromide (10 mg/L; SinaClon Co.) and was visualized using a UV transilluminator (BioDoc-It; UVP, USA).

Davoodabadi A., Abbaszadeh M., Oloomi M, & Bouzari S. (2015). Phenotypic and Genotypic Characterization of Enteroaggregative Escherichia coli Strains Isolated From Diarrheic Children in Iran. Jundishapur Journal of Microbiology, 8(9), e22295.

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Colony Formation Assay for Cells

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Cells previously cultured on a 2D surface (5 × 10³ cells/plate) or within Col-I gels (25 × 10³ cells/gel) for 7 days were collected, dissociated and the cell suspension was cultured in 60 mm culture plates at low cell density (250 cells/plate) for 14 days, with cell media exchange every other day. Cells were washed with PBS, fixed with 4% PF for 10 min, and stained with 0.1% Crystal Violet solution in 20% Methanol for 2 min. Cells were cautiously washed with distilled water and air-dried overnight at room temperature. Digital images of the colonies were acquired in a BioDoc-It (UVP Inc., San Gabriel, CA, USA) imaging system and counted in Fiji software following instructions of a previous report (Cai et al., 2011 (link)).

Valadão I.C., Ralph A.C., Bordeleau F., Dzik L.M., Borbely K.S., Geraldo M.V., Reinhart-King C.A, & Freitas V.M. (2020). High type I collagen density fails to increase breast cancer stem cell phenotype. PeerJ, 8, e9153.

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Gel Electrophoresis Protocol for Molecular Marker Analysis

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The PCR products were separated in 1.7% agarose gels with ethidium bromide and 1× TBE buffer. The gels were run at 80 V for 7 h (3.2 V/cm) and photographed by BioDoc-It (UVP) (Figures S2-S6, Supplementary Material). Bands were estimated using 100 bp DNA Ladder (Solis Biodyne) and scored for their presence (1) or absence (0). Only the repeatable marker data based on polymorphic bands were taken into account during statistical processing. Unreliable bands with a wide range of band intensities (e.g., PCR artifacts) were identified by repeatability tests and excluded from the study (Figure 1). In addition, poorly separated and monomorphic bands of the total frequency above 1-(3/N), where N is total number of individuals, were excluded from the estimation due to the potential for upward bias of diversity estimates [27] (link). The whole process of gel electrophoresis interpretation was done two times by a single person to reduce subjective bias.

Klobučník M., Galgóci M., Gömöry D., & Kormuťák A. (2022). Molecular Insight into Genetic Structure and Diversity of Putative Hybrid Swarms of Pinus sylvestris × P. mugo in Slovakia. Forests, 13(2), 205-205.

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