Ofloxacin

Manufactured by Sangon

Sourced in China

Ofloxacin is a laboratory reagent used as a fluoroquinolone antibiotic. It is commonly used in research and analytical applications.

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Lab products found in correlation

4 protocols using ofloxacin

Isolation and Evaluation of Bacterial Persisters

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Persisters were generated and isolated,
as described by Allison.^{48 (link)} Briefly, a single
clone of Ab4 was grown overnight to saturation. Samples were collected,
subsequently washed with saline 3 times, and then subjected to centrifugation
at 8000 rpm for 5 min. The resulting pellets were resuspended in PBS
at an OD₆₀₀ of 0.8 and incubated with increasing concentrations
(0–960 μg/mL) of ofloxacin (Sangon Biotech) for 4 h.
The remaining cells that could not be eradicated by increasing the
concentration of ofloxacin were persisters. After washing with saline
three times, the persisters were resuspended in M9 medium and adjusted
OD₆₀₀ = 0.2. 5 mL of the persisters plus Meropenem with
or without ATP was added, and the samples were incubated at 37 °C
for 6 h. 100 μL aliquots of the samples were removed and serially
diluted with saline solution. 5 μL aliquots of the diluted samples
were spot-plated onto LB agar plates to count the colonies and determine
viability.

Li X., Feng D., Zhou J., Wu W., Zheng W., Gan W., Jiang M., Li H., Peng X, & Zhang T. (2023). Metabolomics Method in Understanding and Sensitizing Carbapenem-Resistant Acinetobacter baumannii to Meropenem. ACS Infectious Diseases, 10(1), 184-195.

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Antibiotic Resistance in S. epidermidis

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Referred study and our pre-experiments show that S. epidermidis can be isolated from the human intestine tract and colostrum, and drug resistance is high [23 (link)]. S. epidermidis was selected for traceability of ARGs because of its high abundance, isolation and resistance rate in the samples. S. epidermidis from different sources was tested for resistance to 10 antibiotics using the K-B method or by determining the minimal inhibitory concentration according to the standards issued by CLSI in 2012 (https://clsi.org/). The 10 antibiotics were ampicillin, cefotaxime, penicillin, tetracycline, erythromycin, kanamycin, vancomycin, ofloxacin, chloramphenicol and trimethoprim (Sangon Biotech, Shanghai, China).

Zhang K., Jin M., Yang D., Shen Z., Liu W., Yin J., Yang Z., Wang H., Shi D., Yang J., Li H., Chen Y., Gao Z., Qiu Z., Shi H, & Li J.W. (2022). Antibiotic resistance genes in gut of breast-fed neonates born by caesarean section originate from breast milk and hospital ward air. BMC Microbiology, 22, 36.

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Bacterial Hemolysis and Enzyme Assays

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Hemolysin production was detected using Columbia agar plates supplemented with 5% of sheep blood (Amersco, Solon, OH, USA). The presence of α or β-hemolysis was assessed by the formation of clear or greenish zones around the colonies, respectively.
Enzyme activity was measured using the commercially-available, semi-quantitative API-ZYM system (BioMérieux, Montreal, QC) as previously described. According to the manufacturer’s instructions, cell suspension was adjusted to McFarland standards 5. Then 65 μL of cell suspension were added into each well of the API-ZYM strip and were incubated at 37 °C for 4 h in anaerobic conditions. The results were graded based on the amount of from substrate hydrolyzed on a scale from 0 (no activity) to 40 (or ≥ 40 nM).
For antibiotic susceptibility testing, LAB strains (10⁸ CFU/mL) were inoculated onto MRS soft agar. Commercial antibiotic discs (amoxicillin, penicillin, tetracycline, erythromycin, gentamicin, clindamycin, and ofloxacin, provided by Sangon Biotech (Shanghai) Co., Ltd) were placed onto the agar and incubated at 37 °C for 24 h. Resistance or sensitivity was assessed according to the CLSI/NCCLS standard.

Jia G., Liu X., Zhi A., Li J., Wu Y, & Zhang Y. (2021). Characterization and Selection of Lactobacillus plantarum and Lactobacillus paracasei for prevention of oral bacterial infections from Chinese pickle. AMB Express, 11, 84.

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Antimicrobial Susceptibility Testing Protocol

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According to the requirements of the Clinical and Laboratory Standard Institute (CLSI), the antimicrobial susceptibility changes of the strains were detected by using Mueller–Hinton (MH) (hopebiol, Qingdao, China) medium dilution antimicrobial susceptibility tests. The concentration that completely inhibits the growth of the strain is defined as the minimum inhibitory concentration (MIC). The experiment was repeated 3 times.The stock solutions of the following antibiotics were prepared, and their classes are listed in parentheses: ofloxacin (fluoroquinolone, Sangon) at 20 mg/mL; erythromycin (macrolide, Sangon) at 30 mg/mL; Kanamycin (aminoglycoside, Sangon) at 50mg/mL; penicillin G (β-lactams, Sangon) at 100mg/mL.
Based on previous methods (Yu et al., 2020 (link)), the antibiotic susceptibility of the strains was determined by antibacterial activity assays. The overnight cultured strains were diluted into fresh MH containing 30 μg/mL chloramphenicol and incubate in 37°C for 2 h with shaking. After incubation, different antibiotics (final concentration 1/2MIC) were added separately and incubate for another 2 h. Then, they were diluted continuously by 10-fold and three appropriate dilutions (0.1 mL) were dropped onto LB agar plates and colony counts (CFU/mL) were counted after incubation at 37°C. The experiments were repeated 3 times.

Ma K., Chinelo O.R., Gu M., Kong F., Jiang Y., Wang H, & Xue T. (2024). Role of ArcA in the regulation of antibiotic sensitivity in avian pathogenic Escherichia coli. Poultry Science, 103(6), 103686.

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