3 4 5 dimethylthiazol 2 yl 2 5 diphenyl tetrazolium bromide mtt dulbecco s modified eagle s medium dmem eagle s minimum essential medium emem fetal bovine serum

Naringin was purchased from Sigma-Aldrich (Bangalore, India). Acrysol K-140, Acrysol K-150 (propylene glycol-Polyoxyl 40-hydrogenated castor oil) and Kelcogel CG-HA were gifted by Coral Pharma Chem. (Ahmedabad, India) and Signet Chem Corporation (Mumbai, India). Capmul MCM (Glyceryl caprylate/caprate) and Captex 355 were gifts from Abitech Corporation, Columbus, OH, USA. Capryol 90 (Proyle glycol monocarylate), Labrafac CC (Caprylic caric triglyceride) and Labrasol (Caprylocaproyl macrogol-8 glycerides) were gifted by Gattefosse India Pvt. Ltd. (Mumbai, India). Tween 20 and Tween 80 were purchased from Molychem (Mumbai, India). Cremophor EL and Cremophor RH 40 were received as gift sample from BASF (Mumbai, India). Polyethylene glycol 400 and propylene glycol were purchased from LobaChemie Ltd. (Mumbai, India). Ethanol, sodium chloride, potassium chloride and calcium chloride were purchased from Research Lab Fine Chem Industries (Mumbai, India). All these chemicals were of analytical grade and used without any further purification. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Dulbecco’s Modified Eagle’s medium (DMEM), Eagle’s Minimum Essential Medium (EMEM), Fetal bovine serum, Antibiotic solution (100×), Trypsin-EDTA Solution and Hank’s Balanced Salt Solution (HBSS) were purchased from Himedia, Mumbai, India.

Chrysin was purchased from Sigma-Aldrich, Inc., India. Acrysol^® K-140 and Acrysol^® K-150 (propylene glycol–polyoxyl 40–hydrogenated castor oil) were gifted by Coral Pharma Chem (Ahmedabad, India). Capmul^® MCM (glyceryl caprylate/caprate) and Captex^® 355 were gift samples from Abitech Corporation, OH, USA. Capryol^® 90 (propylene glycol monocaprylate), Labrafac^® CC (caprylic capric triglyceride), and Labrasol^® (caprylocaproyl macrogol-8 glycerides) were gifted by Gattefosse India Pvt., Ltd. (Mumbai, India). Tween^® (polysorbate) 20 and Tween^® (polysorbate) 80 were purchased from Molychem (Mumbai, India). Cremophor^® EL, Cremophor^® RH 40, and Pluronic^® F127 were received as gift samples from BASF (Mumbai, India). Polyethylene glycol 400 and propylene glycol were purchased from Loba Chemie, Ltd. (Mumbai, India). Ethanol (96% v/v) was purchased from Research Lab Fine Chem Industries (Mumbai, India). All these chemicals were of analytical grade and used without any further purification. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Dulbecco’s modified Eagle’s medium (DMEM), Eagle’s minimum essential medium (EMEM), fetal bovine serum, antibiotic solution (100X), trypsin-EDTA solution, and Hank’s balanced salt solution (HBSS) were purchased from Himedia, India.