Mouse anti β actin ab8226

Manufactured by Abcam

Mouse anti-β-actin (ab8226) is a primary antibody that specifically recognizes the β-actin protein. It is commonly used as a loading control in Western blotting experiments.

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Lab products found in correlation

Rabbit anti erk, by Cell Signaling Technology (1 mentions) Horseradish peroxidase conjugated goat anti rabbit igg, by Merck Group (1 mentions) Alexa fluor 647 conjugated goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 conjugated goat anti mouse, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase conjugated sheep anti mouse igg, by Jackson ImmunoResearch (1 mentions) Alexa fluor 568 conjugated goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647 conjugated goat anti mouse, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 conjugated goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Irdye 800cw goat anti mouse 926 32210, by LI COR (1 mentions) Phospho mapk family antibody sampler kit, by Cell Signaling Technology (1 mentions) Ab24574, by Abcam (1 mentions) Alexa fluor conjugates, by Thermo Fisher Scientific (1 mentions)

3 protocols using mouse anti β actin ab8226

Immunodetection of Membrane Proteins

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Primary antibodies included rabbit anti-MUC1 C-terminus CT-1 [49 (link)], Armenian hamster anti-MUC1 C-terminus CT-2 (MH1, ProSci Inc., Poway, CA), mouse anti-EGFR (H9B4, Thermo Scientific), mouse anti-EGFR (H11, Thermo Scientific), mouse anti-β-actin (ab8226, Abcam, Cambridge, UK), rabbit anti-EGFR-phospho-Y1068 (Invitrogen), rabbit anti-ERK (Cell Signaling Technology, Danvers, MA), rabbit anti-phospho-ERK (E10, Cell Signaling Technology), and rabbit anti-Ki67 (Novus Biologicals Inc., Littleton, CO). Secondary antibodies for western blotting included horseradish peroxidase-conjugated sheep anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA) and horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma-Aldrich, St. Louis, MO). Secondary antibodies for immunofluorescence included AlexaFluor-488-conjugated goat anti-mouse (Life Technologies, Carlsbad, CA), AlexaFluor-488-conjugated goat anti-rabbit (Life Technologies), AlexaFluor-568-conjugated goat anti-rabbit (Life Technologies), AlexaFluor-647-conjugated goat anti-Armenian hamster (Fisher Scientific), AlexaFluor-647-conjugated goat anti-rabbit (Life Technologies) and AlexaFluor-647-conjugated goat anti-mouse (Life Technologies).

Engel B.J., Bowser J.L., Broaddus R.R, & Carson D.D. (2016). MUC1 stimulates EGFR expression and function in endometrial cancer. Oncotarget, 7(22), 32796-32809.

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Lipid Extraction and LC-MS Analysis of Bioactive Lipids

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Ammonium formate (516961), ammonium acetate (372331), ammonium hydroxide (338818) and lipopolysaccharides from E. coli 0111:B4 (L4391) were from Sigma-Aldrich. Formic acid (06440) was from Fluka. Water (365-4), methanol (230-4), isopropanol (323-4), acetonitrile (017-4) and chloroform (049-1L) for lipid extraction and LC–MS were from Honeywell Burdick & Jackson. C4 silica packing material for precolumn was from Western Analytical Products (214TPB1520) and C18 silica packing material for precolumn was from VWR (53501-270). 6-keto-PGF_1α (10007219), 6, 15-diketo-13, 14-dihydro-PGF_1α (15270), 20-hydroxy-PGF_2α (16950), thromboxane B₂ (19030), 19(R)-hydroxy-PGF_2α (16910) and 19(R)-hydroxy-PGE₁ (13910) were from Cayman Chemical. Mouse anti-COX-1 (ab695), rabbit anti-COX-2 (ab15191) and mouse anti-β-actin (ab8226) antibodies were from Abcam. NF-κB Pathway Sampler Kit (9936), MAPK Family Antibody Sampler Kit (9926) and phospho-MAPK Family Antibody Sampler Kit (9910) were from Cell Signaling Technology. Rabbit anti-BAX (sc-493) and rabbit anti-JNK1/3 (sc-474) antibodies were from Santa Cruz Biotechnology. IRDye 680LT donkey anti-rabbit (926-68023), IRDye 800CW goat anti-rabbit (926-32211) and IRDye 800CW goat anti-mouse (926-32210) antibodies were from LI-COR Biosciences.

Zhang T., Walensky L.D, & Saghatelian A. (2015). A non-apoptotic role for BAX and BAK in eicosanoid metabolism. ACS chemical biology, 10(6), 1398-1403.

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Characterization of EFA6A Antibody

Cited 1 time

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Guinea pig polyclonal EFA6A (1626; 1:400) antibody was a gift from Prof. Eunjoon Kim (Daejeon, South Korea) and was previously characterised (Choi et al., 2006 (link)) and used in independent studies (Raemaekers et al., 2012 (link); Sannerud et al., 2011 (link)). Other primary antibodies were: mouse anti-neurofascin clone A12/18, NeuroMab (RRID:AB_10671311), 1:200; rabbit anti-Rab11 71-5300, Thermo, 1:50; rabbit anti-ARF6 ab77581, Abcam, 1:100; rabbit anti-GST ab19256, Abcam, 1:400; mouse anti-FLAG ab18230, Abcam, 1:750; mouse anti-β-actin ab8226, Abcam, 1:1000; rabbit anti-tRFP ab233, Evrogen, 1:1000; anti-integrin ß1 clone EP1041Y, 04-1109, Millipore, 1:100; and anti-pan-axonal neurofilaments mouse monoclocal SMI312, ab24574, Abcam, 1:800. ACAP1 was detected with goat anti-centaurin β1 (ab15903, Abcam) at 1:50. Secondary antibodies were Alexa Fluor conjugates from Thermo used at 1:1000. Secondary antibodies for western blotting were horseradish peroxidase (HRP) conjugates from GE Life Sciences used at 1:10,000.

Eva R., Koseki H., Kanamarlapudi V, & Fawcett J.W. (2017). EFA6 regulates selective polarised transport and axon regeneration from the axon initial segment. Journal of Cell Science, 130(21), 3663-3675.

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