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Total rna extraction kit

Manufactured by Agilent Technologies
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The Total RNA Extraction Kit is a laboratory tool designed to efficiently isolate and purify total RNA from a variety of biological samples. It utilizes a specialized protocol and reagents to ensure high-quality RNA extraction, suitable for downstream applications such as reverse transcription and gene expression analysis.

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Lab products found in correlation

Cfx connect, by Bio-Rad (4 mentions) Scan drop 100, by Analytik Jena (3 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Itaq universal sybr green supermix, by Bio-Rad (1 mentions) Iq sybr green supermix, by Bio-Rad (1 mentions)

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RNA extraction kit (9 citations)

4 protocols using total rna extraction kit

1

Quantitative RNA Expression Profiling

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Total RNA from renal tissues was isolated using a total RNA extraction Kit (Biotek, Winooski, VT, USA) according to the experimental protocols. Then, the concentration of mRNA was measured using a ScanDrop 100 (Analytik Jena, Thuringia, Germany) determiner. After reverse transcription, quantitative real-time PCR was performed using the fast qPCR kit (Kapa Biosystems, Foster, CA, USA) in a PCR system (CFX Connect; Bio-Rad, Hercules, CA, USA). Primer sequences are listed in Table 1. Relative gene expression was normalized with GAPDH and calculated using the 2−ΔΔCt method.
Shi M., Zeng X., Guo F., Huang R., Feng Y., Ma L., Zhou L, & Fu P. (2017). Anti-Inflammatory Pyranochalcone Derivative Attenuates LPS-Induced Acute Kidney Injury via Inhibiting TLR4/NF-κB Pathway. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(10), 1683.
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2

Quantifying Renal Inflammatory Markers

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Total RNA was obtained from frozen kidney tissue using the total RNA extraction Kit (BioTek, Winooski, VT, United States), and reverse transcription was performed using PrimeScript RT Reagent Kit (Takara Bio, Inc., Otsu, Japan) according to protocols. Reactions of PCR amplification were quantified using the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Inc.) in a PCR system (CFX Connect; Bio-Rad, Hercules, CA, United States). All results were presented with relative expression levels normalized to GAPDH. Primer sequences: IL-6 (Mouse): Forward: 5'-ACAACCACGGCCTTCCCTACTT-3', Reverse: 5'-CACGATTTCCCAGAGAACATGTG-3'; TNF-α (Mouse): Forward: 5'-ACCCTCACACTCAGATCATCTTC-3', Reverse: 5'-TGGTGGTTTGCTACGACGT-3'; NGAL(Mouse): Forward: 5'-GCAGGTGGTACGTTGTGGG-3', Reverse: 5'-CTCTTGTAGCTCATAGATGGTGC-3'.
Liu J., Cui X., Guo F., Li X., Li L., Pan J., Tao S., Huang R., Feng Y., Ma L, & Fu P. (2019). 2-methylquinazoline derivative F7 as a potent and selective HDAC6 inhibitor protected against rhabdomyolysis-induced acute kidney injury. PLoS ONE, 14(10), e0224158.
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3

Quantitative PCR Analysis of Renal RNA

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Total RNA from renal tissues was extracted using a total RNA extraction Kit (BioTek, Winooski, VT, United States) according to the protocols. The concentration of mRNA was tested using a Scan Drop 100 (Analytik Jena, Thuringia, Germany) determiner. Quantitative real-time PCR was performed after reverse transcription by using the iQ SYBR Green Supermix (Bio-Rad, Hercules, CA, United States) in a PCR system (CFX Connect; Bio-Rad, Hercules, CA, United States). Relative expression levels were normalized to GAPDH (Supplementary Table S2).
Huang R., Shi M., Guo F., Feng Y., Feng Y., Liu J., Li L., Liang Y., Xiang J., Lei S., Ma L, & Fu P. (2018). Pharmacological Inhibition of Fatty Acid-Binding Protein 4 (FABP4) Protects Against Rhabdomyolysis-Induced Acute Kidney Injury. Frontiers in Pharmacology, 9, 917.
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4

Quantification of FABP4 Expression in Renal Tissues

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Total RNA from renal tissues was extracted using a total RNA extraction Kit (Biotek, Winooski, VT, USA) according to the protocols. The concentration of mRNA was tested using a Scan Drop 100 (Analytik Jena, Thuringia, Germany) determiner. Quantitative real-time PCR was performed after reverse transcription by using the fast qPCR kit (Kapa Biosystems, Foster, CA, USA) in a PCR system (CFX Connect; Bio-Rad, Hercules, CA, USA). Relative expression levels of FABP4 were normalized to β-actin. The primer sequences were: FABP4 – forward, 5′-AAACACCGAGATTTCCTT-3′ reverse, 5′-TTATGATGCTCTTCACCTT-3′; β-actin - forward, 5′-TATGGAATCCTGTGGCATC-3′ reverse, 5′-GTGTTGGCATAGAGGTCTT-3′.
Shi M., Huang R., Guo F., Li L., Feng Y., Wei Z., Zhou L., Ma L, & Fu P. (2018). Pharmacological inhibition of fatty acid-binding protein 4 (FABP4) protects against renal ischemia-reperfusion injury. RSC Advances, 8(27), 15207-15214.
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